<abstract xmlns="http://www.w3.org/1999/xhtml">
<p>Pollen contains vegetative and generative cells that influence the seed set. Accurate pollen assessments help understand the breeding biology of oilseed rape. In order to evaluate the number of pollen grains in a large number of samples, an effective method should be designed. A new method was established to evaluate the pollen counts of five oilseed rape cultivars. This method mainly relies on 5% sucrose + 0.1% agar solution to suspend the pollen and uses a standard curve corresponding to the absorbance and the number of pollen grains to estimate the number of pollen grains. The linear fit formula of this standard curve was precision, and the R<sup>2</sup> value between the pollen count and absorbance reached 0.998. Furthermore, the variation in the pollen counts at three flowering stages and on two kinds of stamens was assessed. The pollen count per flower varied significantly among oilseed rape cultivars and flowering stages (GLM: p&lt;0.001). Moreover, the number of pollen grains on long-stamen anthers was significantly higher than that on short-stamen anthers (GLM: p&lt;0.001). In conclusion, we established a rapid, accurate method for quantifying pollen grains based on absorbance determined in a liquid suspension by light spectroscopy, which is a feasible method. In addition, the number of pollen grains under different physiological conditions also provides basic data for oilseed rape breeding.</p>
</abstract>

Pollen contains vegetative and generative cells that influence the seed set. Accurate pollen assessments help understand the breeding biology of oilseed rape. In order to evaluate the number of pollen grains in a large number of samples, an effective method should be designed. A new method was established to evaluate the pollen counts of five oilseed rape cultivars. This method mainly relies on 5% sucrose + 0.1% agar solution to suspend the pollen and uses a standard curve corresponding to the absorbance and the number of pollen grains to estimate the number of pollen grains. The linear fit formula of this standard curve was precision, and the R2 value between the pollen count and absorbance reached 0.998. Furthermore, the variation in the pollen counts at three flowering stages and on two kinds of stamens was assessed. The pollen count per flower varied significantly among oilseed rape cultivars and flowering stages (GLM: p<0.001). Moreover, the number of pollen grains on long-stamen anthers was significantly higher than that on short-stamen anthers (GLM: p<0.001). In conclusion, we established a rapid, accurate method for quantifying pollen grains based on absorbance determined in a liquid suspension by light spectroscopy, which is a feasible method. In addition, the number of pollen grains under different physiological conditions also provides basic data for oilseed rape breeding.

Pollen Count Dynamics in Rapeseed Stamens in Early Spring

Key Laboratory for Insect-Pollinator Biology of the Ministry of Agriculture and Rural Affairs

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

{"article-title":"Pollen Count Dynamics in Rapeseed Stamens in Early Spring"}

Pollen contains vegetative and generative cells that influence the seed set. Accurate pollen assessments help understand the breeding biology of oilseed rape....

Pollen Count Dynamics in Rapeseed Stamens in Early Spring

Article Category: Original Article

Publié en ligne: 22 déc. 2023

Pages: 103 - 114

Reçu: 17 avr. 2023

Accepté: 09 oct. 2023

DOI: https://doi.org/10.2478/jas-2023-0008

Mots clés
oilseed rape, pollen, pollen counting, pollen deposition

© 2023 Zhijun Wei et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Pollen Count Dynamics in Rapeseed Stamens in Early Spring

Article Category: Original Article

Publié en ligne: 22 déc. 2023

Pages: 103 - 114

Reçu: 17 avr. 2023

Accepté: 09 oct. 2023

DOI: https://doi.org/10.2478/jas-2023-0008

Mots clésoilseed rape, pollen, pollen counting, pollen deposition

© 2023 Zhijun Wei et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Mots clés
oilseed rape, pollen, pollen counting, pollen deposition