<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The aim of the study was to analyse the intestinal microbiota of honey bees (<italic>Apis mellifera</italic>) treated with amitraz. In the present study, the microbiological profile of the honey bee intestines showed minor changes in the microbiota following the application of amitraz. A comparison of the numbers of bacteria and fungi revealed a positive downward trend in the number of fungi. The number of decreased bacteria, included <italic>E. coli</italic> and especially <italic>Clostridium</italic> spp., which were not isolated from the intestines of bees treated with amitraz. The number of lactic acid bacteria before and after treatment was at a similar level of 8.3 log cfu/g of intestinal contents.</p>
</abstract>

The aim of the study was to analyse the intestinal microbiota of honey bees (Apis mellifera) treated with amitraz. In the present study, the microbiological profile of the honey bee intestines showed minor changes in the microbiota following the application of amitraz. A comparison of the numbers of bacteria and fungi revealed a positive downward trend in the number of fungi. The number of decreased bacteria, included E. coli and especially Clostridium spp., which were not isolated from the intestines of bees treated with amitraz. The number of lactic acid bacteria before and after treatment was at a similar level of 8.3 log cfu/g of intestinal contents.

Intestinal Microbiota of Honey Bees (<italic>Apis mellifera</italic>) Treated with Amitraz

Department of Animal Hygiene and Environmental Hazards, Faculty of Animal Sciences and Bioeconomy

Subdepartment of Apidologie, Institute of Biological Basis of Animal Production; Faculty of Animal Sciences and Bioeconomy

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Intestinal Microbiota of Honey Bees () Treated with Amitraz

The aim of the study was to analyse the intestinal microbiota of honey bees (Apis mellifera) treated with amitraz. In the present study, the microbiological...

Group of microbes	1^st sampling		2^nd sampling		p-value
Group of microbes	M	SD	M	SD	p-value
Bacteria	1.0×10⁸	1.3×10⁷	1.1×10⁸	6.2×10⁷	0.837
E. coli	2.2×10⁸	3.3×10⁷	6.3×10⁷	5.3×10⁶	0.314
Clostridium spp.	7.9×10³	1.1×10²	0.0	0.0	0.143
Lactic acid bacteria	2.2×10⁸	4.9×10⁷	1.9×10⁸	1.0×10⁷	0.887
Bifidobacterium spp.	1.7×10⁷	1.3×10⁷	4.4×10⁶	4.2×10⁵	0.069
Fungi	2.9×10⁷	5.6×10⁷	2.0×10³	1.8×10²	0.268

Group of microbes	1^st sampling		2^nd sampling		p-value

	M	SD	M	SD
Bacteria	7.0×10¹	3.3×10¹	2.8×10²	4.7×10¹	0.0001*
Fungi	7.4×10¹	4.3×10¹	2.9×10²	1.1×10²	0.003*

Group of microbes	1^st sampling		2^nd sampling		p-value
Group of microbes	M	SD	M	SD	p-value
Top bar
Bacteria	1.2×10³	1.9×10²	2.3×10¹	1.7×10¹	0.212
E. coli	9.8×10¹	2.1×10²	0.0	0.0	0.313
Total fungi	1.0×10³	1.1×10²	3.3	4.7	0.064
Yeasts	1.6	3.7	0.0	0.0	0.341

Honeycomb
Bacteria	6.7	7.5	6.7	1.1×10¹	1.000
E. coli	0.0	0.0	0.0	0.0	-
Total fungi	1.7	3.7	1.1×10²	2.4×10¹	0.340
Yeasts	0.0	0.0	6.8×10¹	1.5×10¹	0.341

Hive wall
Bacteria	1.2×10¹	2.2×10¹	5.0	5.0	0.522
E. coli	0.0	0.0	0.0	0.0	-
Total fungi	5.0	1.1×10¹	0.0	0.0	0.341
Yeasts	0.0	0.0	0.0	0.0	-

Intestinal Microbiota of Honey Bees (Apis mellifera) Treated with Amitraz

Article Category: Original Article

Publié en ligne: 27 déc. 2022

Pages: 199 - 207

Reçu: 03 août 2022

Accepté: 23 nov. 2022

DOI: https://doi.org/10.2478/jas-2022-0015

Mots clés
amitraz, microbiota, varroa treatment

© 2022 Anna Żebracka et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1

Fig. 2

Total microbial counts in intestinal homogenates [cfu/g]

Total microbial count in air samples [cfu/m3]

Total microbial count in samples from swabs [cfu/100 cm2]

Intestinal Microbiota of Honey Bees (Apis mellifera) Treated with Amitraz

Article Category: Original Article

Publié en ligne: 27 déc. 2022

Pages: 199 - 207

Reçu: 03 août 2022

Accepté: 23 nov. 2022

DOI: https://doi.org/10.2478/jas-2022-0015

Mots clésamitraz, microbiota, varroa treatment

© 2022 Anna Żebracka et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1

Fig. 2

Total microbial counts in intestinal homogenates [cfu/g]

Total microbial count in air samples [cfu/m3]

Total microbial count in samples from swabs [cfu/100 cm2]

Mots clés
amitraz, microbiota, varroa treatment