<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Body-surface chemical compounds were studied in 1-day-old nest workers and foragers both in Buckfast and Caucasian bees. The workers of these two age-castes were sampled twice in each of two consecutive years. Body-surface lipids were determined by means of gas chromatography, with a GCQ mass spectrometer. Protein concentrations and activities on the body surface were examined in bee cuticle rinsings obtained from worker bees according to the methods of Lowry, of Anson, and of Lee and Lin. Protease and protease inhibitor activities were determined. Polyacrylamide gel electrophoresis was performed. Caucasian bees, particularly foragers, had more lipids, but Buckfast bees (two age-castes) had more proteins on their body surfaces. A total of 17 alkane types (C17 - C33), 13 alkene types (C21 - C33), 21 esters (C12 - C32), and a phenol (C14) were detected in both races. Alkene C33 was detected only in Caucasian bees. More alkanes, esters, and phenols were found in Caucasian 1-day-old nest workers and foragers than in these age-castes of Buckfast bees. The protein concentration and protease inhibitor activities were lower in Caucasian bees that had higher protease activities. These values corresponded with specific numbers and widths of the electrophoretic bands.</p></abstract>

Body-surface chemical compounds were studied in 1-day-old nest workers and foragers both in Buckfast and Caucasian bees. The workers of these two age-castes were sampled twice in each of two consecutive years. Body-surface lipids were determined by means of gas chromatography, with a GCQ mass spectrometer. Protein concentrations and activities on the body surface were examined in bee cuticle rinsings obtained from worker bees according to the methods of Lowry, of Anson, and of Lee and Lin. Protease and protease inhibitor activities were determined. Polyacrylamide gel electrophoresis was performed. Caucasian bees, particularly foragers, had more lipids, but Buckfast bees (two age-castes) had more proteins on their body surfaces. A total of 17 alkane types (C17 - C33), 13 alkene types (C21 - C33), 21 esters (C12 - C32), and a phenol (C14) were detected in both races. Alkene C33 was detected only in Caucasian bees. More alkanes, esters, and phenols were found in Caucasian 1-day-old nest workers and foragers than in these age-castes of Buckfast bees. The protein concentration and protease inhibitor activities were lower in Caucasian bees that had higher protease activities. These values corresponded with specific numbers and widths of the electrophoretic bands.

Body-Surface Compounds in Buckfast and Caucasian Honey Bee Workers (Apis Mellifera)

Department of Biological Basis of Animal Production, Faculty of Biology and Animal Breeding, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland

Department of Zoology, Institute of Biology and Biochemistry Museum Zoological/Laboratory, Faculty of Biology and Biotechnology, Akademicka 19, 20-033 Lublin, Poland

Journal of Apicultural Science

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Body-surface chemical compounds were studied in 1-day-old nest workers and foragers both in Buckfast and Caucasian bees. The workers of these two age-castes...

Body-Surface Compounds in Buckfast and Caucasian Honey Bee Workers (Apis Mellifera)

Publié en ligne: 27 mai 2014

Pages: 5 - 15

Reçu: 26 nov. 2012

Accepté: 21 févr. 2014

DOI: https://doi.org/10.2478/jas-2014-0001

Mots clés
Apis mellifera, chromatography (GC/MS ), cuticle surface, lipid, protein, proteolysis

© by Aneta Strachecka

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Body-Surface Compounds in Buckfast and Caucasian Honey Bee Workers (Apis Mellifera)

Publié en ligne: 27 mai 2014

Pages: 5 - 15

Reçu: 26 nov. 2012

Accepté: 21 févr. 2014

DOI: https://doi.org/10.2478/jas-2014-0001

Mots clésApis mellifera, chromatography (GC/MS ), cuticle surface, lipid, protein, proteolysis

© by Aneta Strachecka

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Mots clés
Apis mellifera, chromatography (GC/MS ), cuticle surface, lipid, protein, proteolysis