<abstract xmlns="http://www.w3.org/1999/xhtml"><p> A comparison was done of the volume of semen and viability of spermatozoa collected from drones at ages 15, 20, 25 and 30 days. The drones originated from different queens and were reared in different environments. Semen volume was determined by measuring the filled length of a capillary. Percentages of live and dead spermatozoa were determined by SYBR-14/propidium iodide fluorescence staining and flow cytometry. The volume of semen collected from drones ranged from 0.5 to 1.3 μL. The mean volume of semen significantly decreased with drone age. Sperm viability increased significantly with drone age.</p></abstract>

A comparison was done of the volume of semen and viability of spermatozoa collected from drones at ages 15, 20, 25 and 30 days. The drones originated from different queens and were reared in different environments. Semen volume was determined by measuring the filled length of a capillary. Percentages of live and dead spermatozoa were determined by SYBR-14/propidium iodide fluorescence staining and flow cytometry. The volume of semen collected from drones ranged from 0.5 to 1.3 μL. The mean volume of semen significantly decreased with drone age. Sperm viability increased significantly with drone age.

The Influence of Honey Bee (Apis Mellifera) Drone Age on Volume of Semen and Viability of Spermatozoa

Department of Pomology and Apiculture, Agricultural University, 29 Listopada 54, 31-425 Krakow, Poland

Department of Zoology and Apiculture, West Pomeranian University of Technology, Doktora Judyma 20, 71-466 Szczecin, Poland

Department of Epizootiology and Veterinary Administration with Clinic, University of Environmental and Life Sciences, pl. Grunwaldzki 45; 50-366 Wrocław, Poland

Journal of Apicultural Science

A comparison was done of the volume of semen and viability of spermatozoa collected from drones at ages 15, 20, 25 and 30 days. The drones originated from...

The Influence of Honey Bee (Apis Mellifera) Drone Age on Volume of Semen and Viability of Spermatozoa

Publié en ligne: 05 juin 2013

Pages: 61 - 66

DOI: https://doi.org/10.2478/jas-2013-0007

Mots clés
Apis mellifera, honey bee, drone, age, sperm viability

This content is open access.

The Influence of Honey Bee (Apis Mellifera) Drone Age on Volume of Semen and Viability of Spermatozoa

Publié en ligne: 05 juin 2013

Pages: 61 - 66

DOI: https://doi.org/10.2478/jas-2013-0007

Mots clésApis mellifera, honey bee, drone, age, sperm viability

This content is open access.

Mots clés
Apis mellifera, honey bee, drone, age, sperm viability