<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This study aims to investigate the level of genetic variability of <italic>Fasciola hepatica</italic> flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 <italic>F. hepatica</italic> flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among <italic>F. hepatica</italic> isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of <italic>F. hepatica</italic> are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes’ worldwide distribution.</p></abstract>

This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 F. hepatica flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among F. hepatica isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of F. hepatica are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes’ worldwide distribution.

New insights into the genetic variability of <italic>Fasciola hepatica</italic> (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

New insights into the genetic variability of  (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA

This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic...

New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA

Publié en ligne: 03 sept. 2022

Pages: 152 - 164

Reçu: 19 janv. 2022

Accepté: 14 juil. 2022

DOI: https://doi.org/10.2478/helm-2022-0021

Mots clés
molecular characterization, COI, ITS, phylogeography

© 2022 M. Chaouadi, F. Scarpa, I. Azzena, P. Cossu, K. Harhoura, M. Aissi, F. Tazerouti, G. Garippa, P. Merella, M. Casu, D. Sanna, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Détails du matériel supplémentaire

New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA

Publié en ligne: 03 sept. 2022

Pages: 152 - 164

Reçu: 19 janv. 2022

Accepté: 14 juil. 2022

DOI: https://doi.org/10.2478/helm-2022-0021

Mots clésmolecular characterization, COI, ITS, phylogeography

© 2022 M. Chaouadi, F. Scarpa, I. Azzena, P. Cossu, K. Harhoura, M. Aissi, F. Tazerouti, G. Garippa, P. Merella, M. Casu, D. Sanna, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Détails du matériel supplémentaire

Mots clés
molecular characterization, COI, ITS, phylogeography