Accès libre

A pilot comparative study between serological and genetic investigations in relationship to clinical outcomes on patients with cystic echinococcosis

À propos de cet article

Citez

Fig. 1

Western Blot analysis of patient sera using HF (HF-WB). The lines represent: 1-Positive Control, 2-Negative Control, 3-22 Sera with confirmed CE.
Western Blot analysis of patient sera using HF (HF-WB). The lines represent: 1-Positive Control, 2-Negative Control, 3-22 Sera with confirmed CE.

Fig. 2

CO1, NAD1 and ITS-1 Gene Amplicons. A. CO1 gene amplicons (446 bp). M:DNA Marker, 1:Positive Control, 2-3:Human Isolates, 4:Negative Control (Distilled water) B. NAD1 gene amplicons (378 bp). M:DNA Marker, 1:Positive Control, 2:Negative Control (Distilled water), 3-4:Human Isolates. C. ITS-1 gene amplicons. M:DNA Marker, 1:Positive Control, 2:Negative Control (Distilled water), 3:Human.
CO1, NAD1 and ITS-1 Gene Amplicons. A. CO1 gene amplicons (446 bp). M:DNA Marker, 1:Positive Control, 2-3:Human Isolates, 4:Negative Control (Distilled water) B. NAD1 gene amplicons (378 bp). M:DNA Marker, 1:Positive Control, 2:Negative Control (Distilled water), 3-4:Human Isolates. C. ITS-1 gene amplicons. M:DNA Marker, 1:Positive Control, 2:Negative Control (Distilled water), 3:Human.

Fig. 3

A map of Turkey showing the distribution of 29 CE cases province caused by E. granulosus.
A map of Turkey showing the distribution of 29 CE cases province caused by E. granulosus.

Gender and age, ELISA and Western Blot results, organ localization, molecular identification and clinical symptoms, drug used and dog owner informations of the 29 CE cases.

No of patientGenderAgeProvinceELISA Od value / EvaluationWB bands ( kDa)Organ localisationGenotype of E. granulosus s.s.Drug Used /NADog ownerClinical symptoms
1M29Kütahya2,403/H12,20-22,36,75,90Liver right lobeE. granulosus s.s. (G1-G3)YYMild pain
2M36İzmir2,859/H812,20-22,36,75,90Liver right lobe seg. 6-7E. granulosus s.s. (G1-G3)YNPain, palpable mass, headache
3M12Izmir1,047/M8,12,20-22,36,75,90Liver right lobe posteriorE. granulosus s.s. (G1-G3)YNPain
4F31Bornova/İzmir0,792/M12,20-22,36,75,90Liver right lobe seg. 6-7E. granulosus s.s. (G1-G3)YNPain, vomiting
5M36Bergama/İzmir0,865/M8,12,20-22,36,75,90Liver left lobe seg. 7E. granulosus s.s. (G1-G3)YYPalpable mass, pain, nausea, vomiting,
6M63Karabağlar/İzmir2,069/H8,12,20-22,36,75,90Liver right 4A-BE. granulosus s.s. (G1-G3)YNPain
7M53Izmir0,498/L12, 36 (low), 75, 90Liver seg. 4-5-6E. granulosus s.s. (G1-G3)YNPalpable mass, pain, weakness, nausea
8M23Manisa0,974/M20-22, 36, 75, 90Liver right lobeE. granulosus s.s. (G1-G3)YYPalpable mass, pain
9M42İzmir0,469/L12, 20-22, 36 (low), 75, 90Liver right 4A-BE. granulosus s.s. (G1-G3)YNPain
10M40Buca/İzmir1,032/M20-22, 36, 75, 90Liver hilumE. granulosus s.s. (G1-G3)NYPain
11F30İzmir0,831/M12, 36 (low), 90Liver right 4BE. granulosus s.s. (G1-G3)YYPain, nausea, vomiting
12F13Merkez/Aydın1,569/H12,20-22,36,75,90LiverE. granulosus s.s. (G1-G3)YNPain, nausea, vomiting
13F61Alaşehir/Manisa0,552/L20-22, 36, 75, 90Liver right lobeE. granulosus s.s. (G1-G3)NYAğrı,çarpıntı
14F49Denizli1,058/M8, 20-22, 36, 75, 90LiverE. granulosus s.s. (G1-G3)NYPain, nausea
15M49Söke/Aydın0,936/M8,12,20-22,36LiverE. granulosus s.s. (G1-G3)YNPain, palpitations
16M10Gömeç/Balıkesir0,099/N12, 20-22 (low)LiverE. granulosus s.s. (G1-G3)YYPalpable mass, pain, hepatomegaly, dizziness
17F62Balıkesir0,454/L12,20-22,36,75,90LiverE. granulosus s.s. (G1-G3)YYSevere pain, nausea, vomiting, jaundice
18F19Aydın0,142/N20-22 (low)LiverE. granulosus s.s. (G1-G3)YYPalpable mass, severe pain
19M59Akhisar/Manisa0,944/M36, 75, 90LiverE. granulosus s.s. (G1-G3)YYBack stiffness, severe pain, hepatomegaly
20M30Balıkesir1,018/M20-22, 36, 75, 90LiverE. granulosus s.s. (G1-G3)YNSevere pain, nausea, vomiting, jaundice
21F15Bornova/İzmir0,243/N12,36,75LiverE. granulosus s.s. (G1-G3)YNSevere pain, nausea
22M15Izmir0,551/L8, 20-22, 36, 75, 90LiverE. granulosus s.s. (G1-G3)YNPalpable mass, pain
23F52Menemen/İzmir1,063/M8, 20-22, 36, 75, 90LiverE. granulosus s.s. (G1-G3)YYPain
24M8Ayvalık/Balıkesir0,649/L8,12,20-22,36,75,90LiverE. granulosus s.s. (G1-G3)YYPalpable mass, fever, vomiting
25M26Söke/Aydın0,649/L8,12,20-22,36,75,90LiverE. granulosus s.s. (G1-G3)YYSevere pain, swelling
26M19Akhisar/Manisa1,066/M8,12,20-22,36,75,90LiverE. granulosus s.s. (G1-G3)YNPain
27F10Manisa0,498/L12,20-22LiverE. granulosus s.s. (G1-G3)YNPain, loss of appetite, anemia
28M48Muğla1,262/H8,12,20-22,36,75,90LiverE. granulosus s.s. (G1-G3)YYVomiting, weakness, hepatomegaly
29M10Izmir1,047/M8,12,20-22,36,75,90LiverE. granulosus s.s. (G1-G3)YYPalpable mass, nausea, vomiting

Oligonucleotide primers used in PCR and DNA Sequencing for typing of Echinococcus granulosus.

PrimersGene RegionsNucleotide SequencesSources
MS1NAD1CGTAGGTATGTTGGTTTGTTTGGTSharbatkhori et al., 2009
MS2NAD1CATAATCAAATGGCGTACGATSharbatkhori et al., 2009
JB3CO1TTTTTTGGGCATCCTGAGGTTTATUtuk et al., 2008
JB4.5CO1TAAAGAAAGAACATAATGAAAATGUtuk et al., 2008
BD1ITS-1GTCGTAACAAGGTTTCCGTABowless & McManus, 1993
4SITS-1TCTAGATGCGTTCGAA(G/A)TGTCGATGBowless & McManus, 1993

Age and gender distribution of CE patients.

Patient AgeMaleFemaleTotal
0 – 12415
13 – 20235
21 – 35325
36 +10414
Total191029
eISSN:
1336-9083
Langue:
Anglais