<abstract xmlns="http://www.w3.org/1999/xhtml"><p>This study was carried out to estimate the minimal number of eggs present in adult <italic>E. coelomaticum</italic> uterus. Samples were collected during post-mortem inspection and were submitted to light microscopy (bright field). The length, width, the total area of the parasite, uterus, and eggs were measured. The ImageJ software was used to calculate the area of the different parameters analyzed in this study. It was possible to observe that the uterus corresponds on average to 51.9 % of the total area of the parasite (ranging from 45 to 64 %). The number of eggs present in the uterus of parasites ranged from 5,946 to 15,813. To estimate the number of eggs three scenarios were considered, where the first taken into account the number of whole eggs observed in the image. In the second way to estimate the number of eggs, all the structures were considered (whole eggs and fractions that could be delimited) and compared with manual counting. Finally, in the last scenario, was considered an occupancy rate of 100 % of the uterine area per eggs, since there are overlapping eggs and these cannot be correctly delimited and accounted for. This study describes an important tool for quantifying eggs in a nondestructive manner and aggregate information until then is not explained by other works.</p></abstract>

This study was carried out to estimate the minimal number of eggs present in adult E. coelomaticum uterus. Samples were collected during post-mortem inspection and were submitted to light microscopy (bright field). The length, width, the total area of the parasite, uterus, and eggs were measured. The ImageJ software was used to calculate the area of the different parameters analyzed in this study. It was possible to observe that the uterus corresponds on average to 51.9 % of the total area of the parasite (ranging from 45 to 64 %). The number of eggs present in the uterus of parasites ranged from 5,946 to 15,813. To estimate the number of eggs three scenarios were considered, where the first taken into account the number of whole eggs observed in the image. In the second way to estimate the number of eggs, all the structures were considered (whole eggs and fractions that could be delimited) and compared with manual counting. Finally, in the last scenario, was considered an occupancy rate of 100 % of the uterine area per eggs, since there are overlapping eggs and these cannot be correctly delimited and accounted for. This study describes an important tool for quantifying eggs in a nondestructive manner and aggregate information until then is not explained by other works.

Digital image analysis to estimate the minimum number of <italic>Eurytrema coelomaticum</italic> eggs in the uterus of adult specimens

Faculdade de Ciências Biológicas e da Saúde

Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)

Laboratório de Helmintos Parasitos de Vertebrados – LHPV

Instituto Oswaldo Cruz – IOC, Fundação Oswaldo Cruz – FIOCRUZ

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Digital image analysis to estimate the minimum number of  eggs in the uterus of adult specimens

This study was carried out to estimate the minimal number of eggs present in adult E. coelomaticum uterus. Samples were collected during post-mortem inspection...

Digital image analysis to estimate the minimum number of Eurytrema coelomaticum eggs in the uterus of adult specimens

Publié en ligne: 28 juil. 2018

Pages: 204 - 212

Reçu: 15 nov. 2017

Accepté: 12 mars 2018

DOI: https://doi.org/10.2478/helm-2018-0014

Mots clés
Trematoda, Dicrocoeliidae, ImageJ

© 2018 F. A. da Silva Júnior et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Digital image analysis to estimate the minimum number of Eurytrema coelomaticum eggs in the uterus of adult specimens

Publié en ligne: 28 juil. 2018

Pages: 204 - 212

Reçu: 15 nov. 2017

Accepté: 12 mars 2018

DOI: https://doi.org/10.2478/helm-2018-0014

Mots clésTrematoda, Dicrocoeliidae, ImageJ

© 2018 F. A. da Silva Júnior et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Trematoda, Dicrocoeliidae, ImageJ