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Daily Acute Bouts of Weight-bearing During Hindlimb Unloading Mitigate Disuse-Induced Deficits in Cancellous Bone

Rihana S. Bokhari
Rihana S. Bokhari
, 
Corinne E. Metzger
Corinne E. Metzger
, 
Matthew R. Allen
Matthew R. Allen
 et 
Susan A. Bloomfield
Susan A. Bloomfield
   | 21 juil. 2020
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Article Category: Short Communications
Publié en ligne: 21 juil. 2020
Pages: 2 - 11
DOI: https://doi.org/10.2478/gsr-2018-0006
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© 2018 Rihana S. Bokhari et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Study information. A) Study timeline: Animals acclimated to AIN-93G diet starting at 17 weeks of age. HU animals began HU four weeks later, while HU+WB animals were removed from tail suspension and allowed weight-bearing cage activity for 75 minutes per day. B) HU mouse suspended via tail ring.
Study information. A) Study timeline: Animals acclimated to AIN-93G diet starting at 17 weeks of age. HU animals began HU four weeks later, while HU+WB animals were removed from tail suspension and allowed weight-bearing cage activity for 75 minutes per day. B) HU mouse suspended via tail ring.

Figure 2

Representative images of cancellous bone volume and microarchitecture at the distal femur after 4 weeks for cage control (CC), hindlimb unloaded (HU), and hindlimb unloaded + weight-bearing (HU+WB) mice.
Representative images of cancellous bone volume and microarchitecture at the distal femur after 4 weeks for cage control (CC), hindlimb unloaded (HU), and hindlimb unloaded + weight-bearing (HU+WB) mice.

Figure 3

Body mass and body composition after 4 weeks for cage control (CC), hindlimb unloaded (HU), and hindlimb unloaded + weight-bearing (HU+WB) mice. A) Body mass at the end of HU was lower in HU (−9%) and HU+WB (−7%) mice compared to cage controls (CC). B) Total body BMD was lower in HU (−7%), and HU+WB (−7%) vs. CC. C) Fat mass was lower in HU (−21%) compared to CC and HU+WB. D) Lean mass was lower in HU compared to CC (−5%) with no difference from HU+WB. Groups sharing a letter are not different from one another by one-way ANOVA (p<0.05).
Body mass and body composition after 4 weeks for cage control (CC), hindlimb unloaded (HU), and hindlimb unloaded + weight-bearing (HU+WB) mice. A) Body mass at the end of HU was lower in HU (−9%) and HU+WB (−7%) mice compared to cage controls (CC). B) Total body BMD was lower in HU (−7%), and HU+WB (−7%) vs. CC. C) Fat mass was lower in HU (−21%) compared to CC and HU+WB. D) Lean mass was lower in HU compared to CC (−5%) with no difference from HU+WB. Groups sharing a letter are not different from one another by one-way ANOVA (p<0.05).

Figure 4

Cancellous bone volume and microarchitecture at the distal femur after 4 weeks for cage control (CC), hindlimb unloaded (HU), and hindlimb unloaded + weight-bearing (HU+WB) mice. A) % bone volume/total volume (%BV/TV) was 22% lower in HU compared to cage controls (CC), whereas this value in HU+WB was equivalent to CC mice. B) Trabecular thickness was 9% lower in HU compared to CC and HU+WB. C) Trabecular separation was not different among groups. D) Trabecular number was 13% lower in HU compared to CC, whereas HU+WB was not different than CC mice. Groups sharing a letter are not different from one another by one-way ANOVA (p<0.05)
Cancellous bone volume and microarchitecture at the distal femur after 4 weeks for cage control (CC), hindlimb unloaded (HU), and hindlimb unloaded + weight-bearing (HU+WB) mice. A) % bone volume/total volume (%BV/TV) was 22% lower in HU compared to cage controls (CC), whereas this value in HU+WB was equivalent to CC mice. B) Trabecular thickness was 9% lower in HU compared to CC and HU+WB. C) Trabecular separation was not different among groups. D) Trabecular number was 13% lower in HU compared to CC, whereas HU+WB was not different than CC mice. Groups sharing a letter are not different from one another by one-way ANOVA (p<0.05)

Total body bone mineral density at the start of hindlimb unloading (HU). There were no differences in intermittent weight-bearing (HU+WB) or HU compared to cage controls (CC).

CCHUHU+WB
Total Body Bone Mineral Density (g/cm2) One Day Prior to Hindlimb Unloading0.060 ± 0.0010.055 ± 0.0010.057 ± 0.004
eISSN:
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Langue:
Anglais
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Life Sciences, other, Materials Sciences, Physics
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