PCR Detection of an Eye Anomaly in a Family of Longhaired Collies
Publié en ligne: 30 déc. 2022
Pages: 75 - 81
Reçu: 10 oct. 2022
Accepté: 21 nov. 2022
DOI: https://doi.org/10.2478/fv-2022-0040
Mots clés
© 2022 Holečková B. et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Inherited eye diseases have been the subject of genetic research for many years. This paper focuses on the optimisation of the DNA test based on the polymerase chain reaction (PCR) for the detection of Collie Eye Anomaly (CEA) in dogs. A small family of four longhaired Collies (parents and their daughters) with a confirmed positive clinical ophthalmologic examination of CEA served as the source of affected animals. Both PCR reaction conditions examined were suitable for detecting canine