<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Yeasts from the genus <italic>Malassezia</italic> belongs to normal commensal skin flora of warm-blooded vertebrates. These yeasts may act as opportunistic pathogens and cause skin diseases in humans and animals under certain conditions. The identification of <italic>Malassezia</italic> species is based on the phenotypic or genotypic diagnostics. The methods used for the phenotypic identification is determined by: the growth on Sabouraud agar, growth on selective media (Leeming-Notman agar, Dixon agar, Chrom <italic>Malassezia</italic> agar), the ability to utilise different concentrations of Tween, monitoring of the growth on CEL agar (soil enriched with castor oil) and TE agar (Tween-esculine agar), and the catalase test. The genotypic identification uses molecular methods like: the pulsed field gel electrophoresis (PFGE), random amplified polymorphic DNA (RAPD), amplified fragment lenght polymorphism (AFLP), denaturing gradient gel electrophoresis (DGGE), and the DNA sequence analysis.</p></abstract>

Yeasts from the genus Malassezia belongs to normal commensal skin flora of warm-blooded vertebrates. These yeasts may act as opportunistic pathogens and cause skin diseases in humans and animals under certain conditions. The identification of Malassezia species is based on the phenotypic or genotypic diagnostics. The methods used for the phenotypic identification is determined by: the growth on Sabouraud agar, growth on selective media (Leeming-Notman agar, Dixon agar, Chrom Malassezia agar), the ability to utilise different concentrations of Tween, monitoring of the growth on CEL agar (soil enriched with castor oil) and TE agar (Tween-esculine agar), and the catalase test. The genotypic identification uses molecular methods like: the pulsed field gel electrophoresis (PFGE), random amplified polymorphic DNA (RAPD), amplified fragment lenght polymorphism (AFLP), denaturing gradient gel electrophoresis (DGGE), and the DNA sequence analysis.

Diagnostics of <italic>Malassezia</italic> Species: A Review

Department of Pharmacology and Toxicology

University of Veterinary Medicine and Pharmacy

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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Yeasts from the genus Malassezia belongs to normal commensal skin flora of warm-blooded vertebrates. These yeasts may act as opportunistic pathogens and cause...

Diagnostics of Malassezia Species: A Review

Publié en ligne: 17 juil. 2018

Pages: 19 - 29

Reçu: 19 janv. 2018

Accepté: 04 avr. 2018

DOI: https://doi.org/10.2478/fv-2018-0013

Mots clés
genotypic, identification, phenotypic, yeasts

© 2018 Böhmová, E. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Diagnostics of Malassezia Species: A Review

Publié en ligne: 17 juil. 2018

Pages: 19 - 29

Reçu: 19 janv. 2018

Accepté: 04 avr. 2018

DOI: https://doi.org/10.2478/fv-2018-0013

Mots clésgenotypic, identification, phenotypic, yeasts

© 2018 Böhmová, E. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
genotypic, identification, phenotypic, yeasts