<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Inter primer binding site (iPBS) polymorphism was investigated for common ivy (<italic>Hedera helix</italic>, L.) to obtain the knowledge on genetic diversity in this species. Actually, a very limited information exists about application of DNA markers in <italic>Hedera helix</italic>. Natural and planted European populations of ivy were analysed using an iPBS marker 5′ACCTGGCGTGCCA3′ with a total number of 238 fragments generated. Of these, 86% were polymorphic. There were determined certain attributes of this marker such as the diversity index (DI) and polymorphism information content (PIC). The value of the diversity index was 0.79 and the polymorphic information index was 0.78. The proportion of polymorphisms of the individual amplified loci ranged from 0.32% to 6.98%. Cluster analysis was performed to determine the relationships among the European ivy populations where the distribution in the dendrogram under the habitat specifity was found for the used iPBS marker. We concluded that iPBS was very efficient in analysing the genetic diversity in <italic>Hedera helix</italic>, L. and that this marker can serve as a suitable tool to find genomically specific fingerprints relevant to the factors influencing the distribution of genetic variation.</p></abstract>

Inter primer binding site (iPBS) polymorphism was investigated for common ivy (Hedera helix, L.) to obtain the knowledge on genetic diversity in this species. Actually, a very limited information exists about application of DNA markers in Hedera helix. Natural and planted European populations of ivy were analysed using an iPBS marker 5′ACCTGGCGTGCCA3′ with a total number of 238 fragments generated. Of these, 86% were polymorphic. There were determined certain attributes of this marker such as the diversity index (DI) and polymorphism information content (PIC). The value of the diversity index was 0.79 and the polymorphic information index was 0.78. The proportion of polymorphisms of the individual amplified loci ranged from 0.32% to 6.98%. Cluster analysis was performed to determine the relationships among the European ivy populations where the distribution in the dendrogram under the habitat specifity was found for the used iPBS marker. We concluded that iPBS was very efficient in analysing the genetic diversity in Hedera helix, L. and that this marker can serve as a suitable tool to find genomically specific fingerprints relevant to the factors influencing the distribution of genetic variation.

Habitat-related specifity of iPBS fingerprint in European populations of <italic>Hedera helix</italic> L.

Faculty of Agrobiology and Food Resources

Slovak University of Agriculture in Nitra

Folia Oecologica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Habitat-related specifity of iPBS fingerprint in European populations of  L.

Inter primer binding site (iPBS) polymorphism was investigated for common ivy (Hedera helix, L.) to obtain the knowledge on genetic diversity in this species....

Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L.

Publié en ligne: 27 mai 2019

Pages: 30 - 36

Reçu: 12 sept. 2018

Accepté: 21 mars 2019

DOI: https://doi.org/10.2478/foecol-2019-0005

Mots clés
genetic diversity, L., iPBS, population

© 2019 Jana Žiarovská et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L.

Publié en ligne: 27 mai 2019

Pages: 30 - 36

Reçu: 12 sept. 2018

Accepté: 21 mars 2019

DOI: https://doi.org/10.2478/foecol-2019-0005

Mots clésgenetic diversity, L., iPBS, population

© 2019 Jana Žiarovská et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
genetic diversity, L., iPBS, population