<abstract xmlns="http://www.w3.org/1999/xhtml">The aim of the study was to develop an efficient micropropagation system for <italic>Rosa</italic> ‘Konstancin’, an interspecific hybrid between <italic>R. rugosa</italic> and <italic>R. beggeriana</italic>, whose fruits have high pro-health value. Shoot cultures were initiated from shoot buds collected in May and August from 15-year-old field-grown <italic>Rosa</italic> ‘Konstancin’ shrubs. The effect and interaction of different concentrations of phytohormones, sucrose and iron sources on <italic>in vitro</italic> initiation, multiplication and rooting of shoots were studied.The time of collecting explants from donor plants significantly affected the initiation of shoot culture of <italic>Rosa</italic> ‘Konstancin’. Considerably higher frequency of bud break (100%) was obtained in explants isolated in August as compared to those collected at the end of May (30%). All buds developed into single shoots after 2-4 weeks of growing on the basal Murashige and Skoog medium containing 2.2 µM BAP, 0.3 µM GA3 and 88 mM of sucrose. The highest multiplication rate (4.8 shoots/explant) in a 5-week period was obtained on MS medium containing 50% of nitrogen salts, 3.1 µM BAP, 0.9 µM GA3 and 58 mM sucrose. High rooting frequency (100%) and quality of rooted plantlets was obtained on a medium containing 0.5 µM IBA, 138 µM Fe-EDDHA and 88 mM sucrose. Fe-EDDHA had a beneficial effect on the growth and photosynthetic activity of <italic>Rosa</italic> ‘Konstancin’ plantlets, which were successfully acclimatized <italic>ex vitro</italic>, with a more than 90% survival rate.</abstract>

The aim of the study was to develop an efficient micropropagation system for Rosa ‘Konstancin’, an interspecific hybrid between R. rugosa and R. beggeriana, whose fruits have high pro-health value. Shoot cultures were initiated from shoot buds collected in May and August from 15-year-old field-grown Rosa ‘Konstancin’ shrubs. The effect and interaction of different concentrations of phytohormones, sucrose and iron sources on in vitro initiation, multiplication and rooting of shoots were studied.The time of collecting explants from donor plants significantly affected the initiation of shoot culture of Rosa ‘Konstancin’. Considerably higher frequency of bud break (100%) was obtained in explants isolated in August as compared to those collected at the end of May (30%). All buds developed into single shoots after 2-4 weeks of growing on the basal Murashige and Skoog medium containing 2.2 µM BAP, 0.3 µM GA3 and 88 mM of sucrose. The highest multiplication rate (4.8 shoots/explant) in a 5-week period was obtained on MS medium containing 50% of nitrogen salts, 3.1 µM BAP, 0.9 µM GA3 and 58 mM sucrose. High rooting frequency (100%) and quality of rooted plantlets was obtained on a medium containing 0.5 µM IBA, 138 µM Fe-EDDHA and 88 mM sucrose. Fe-EDDHA had a beneficial effect on the growth and photosynthetic activity of Rosa ‘Konstancin’ plantlets, which were successfully acclimatized ex vitro, with a more than 90% survival rate.

<italic>In vitro</italic> propagation of <italic>Rosa</italic> ‘Konstancin’ (<italic>R. rugosa</italic> × <italic>R. beggeriana</italic>), a plant with high nutritional and pro-health value

Department of Applied Biology, Research Institute of Horticulture

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 propagation of  ‘Konstancin’ ( × ), a plant with high nutritional and pro-health value

The aim of the study was to develop an efficient micropropagation system for Rosa ‘Konstancin’, an interspecific hybrid between R. rugosa and R. beggeriana,...

In vitro propagation of Rosa ‘Konstancin’ (R. rugosa × R. beggeriana), a plant with high nutritional and pro-health value

Publié en ligne: 14 déc. 2018

Pages: 259 - 267

Reçu: 31 août 2017

Accepté: 22 févr. 2018

DOI: https://doi.org/10.2478/fhort-2018-0022

Mots clés
fruit rose, Fe-EDDHA, micropropagation, photosynthetic activity, sucrose

© 2018 Agnieszka Wojtania et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In vitro propagation of Rosa ‘Konstancin’ (R. rugosa × R. beggeriana), a plant with high nutritional and pro-health value

Publié en ligne: 14 déc. 2018

Pages: 259 - 267

Reçu: 31 août 2017

Accepté: 22 févr. 2018

DOI: https://doi.org/10.2478/fhort-2018-0022

Mots clésfruit rose, Fe-EDDHA, micropropagation, photosynthetic activity, sucrose

© 2018 Agnieszka Wojtania et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
fruit rose, Fe-EDDHA, micropropagation, photosynthetic activity, sucrose