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Peculiarities of determining the morphogenesis of plants Corylus avellana L. and Prunus dulcis (Mill.) D.A.Webb in vitro culture

Vyacheslav Matskevych
Vyacheslav Matskevych
, 
Vasyl Yukhnovskyi
Vasyl Yukhnovskyi
, 
Ivan Kimeichuk
Ivan Kimeichuk
, 
Oksana Matskevych
Oksana Matskevych
 et 
Oksana Shyta
Oksana Shyta
   | 13 mars 2023
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Article Category: ORIGINAL ARTICLE
Publié en ligne: 13 mars 2023
Pages: 1 - 14
Reçu: 18 août 2022
Accepté: 18 nov. 2022
DOI: https://doi.org/10.2478/ffp-2023-0001
© 2023 Vyacheslav Matskevych et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

Reduction of biometric dimensions of regenerants of the Jefferson variety during long-term cultivation on a medium with an excess of auxins on 30th day of cultivation (the figures correspond to the passage number)
Reduction of biometric dimensions of regenerants of the Jefferson variety during long-term cultivation on a medium with an excess of auxins on 30th day of cultivation (the figures correspond to the passage number)

Figure 2.

The condition of hazelnut regenerants of the Tonda Gentile Romana variety on a medium with 1.0 mg/l BAP and 0.3 IBA on the 30th day of propagation (the figures correspond to the passage number)
The condition of hazelnut regenerants of the Tonda Gentile Romana variety on a medium with 1.0 mg/l BAP and 0.3 IBA on the 30th day of propagation (the figures correspond to the passage number)

Figure 3.

The condition of regenerants of the hazelnut variety Tonda Gentile Romana on a medium with 0.3 mg/l BAP and 1.5 IBA on the 30th day of propagation (the figures correspond to the passage number)
The condition of regenerants of the hazelnut variety Tonda Gentile Romana on a medium with 0.3 mg/l BAP and 1.5 IBA on the 30th day of propagation (the figures correspond to the passage number)

Figure 4.

Hyperhydration of hazelnut regenerants of the Tonda Gentile Romana variety during passaging on media with the addition of 2.5 mg/l BAP and 1.5 mg/l gibberellin (the figures correspond to the passage number)
Hyperhydration of hazelnut regenerants of the Tonda Gentile Romana variety during passaging on media with the addition of 2.5 mg/l BAP and 1.5 mg/l gibberellin (the figures correspond to the passage number)

Figure 5.

Callus formation in vitro in hazelnut and almond explants with the simultaneous addition of high concentrations of cytokinin BAP and IBA to the nutrient medium: (a) callus formation of hazelnut regenerants of the Tonda Gentile Romana variety on the medium with the addition of 2.5 mg/l BAP and 2.5 mg/l IBA; (b) rosette stem of almond regenerants of variety M41 Alex on the medium with the addition of 1.0 mg/l BAP and 1.0 mg/l IBA; (c) rosette stem and callus formation of almond regenerants of variety M41 Alex on the medium with the addition of 1.5 mg/l BAP and 1.5 mg/l IBA; (d) callus formation of almond regenerants of the M41 Alex variety in the basal part of the cutting explant on the medium with the addition of 2.0 mg/l BAP and 2.0 mg/l IBA; (e) callus formation of almonds of the M41 Alex variety over the entire surface of the cutting explant on the medium with the addition of 2.5 mg/l BAP and 2.5 mg/l IBA
Callus formation in vitro in hazelnut and almond explants with the simultaneous addition of high concentrations of cytokinin BAP and IBA to the nutrient medium: (a) callus formation of hazelnut regenerants of the Tonda Gentile Romana variety on the medium with the addition of 2.5 mg/l BAP and 2.5 mg/l IBA; (b) rosette stem of almond regenerants of variety M41 Alex on the medium with the addition of 1.0 mg/l BAP and 1.0 mg/l IBA; (c) rosette stem and callus formation of almond regenerants of variety M41 Alex on the medium with the addition of 1.5 mg/l BAP and 1.5 mg/l IBA; (d) callus formation of almond regenerants of the M41 Alex variety in the basal part of the cutting explant on the medium with the addition of 2.0 mg/l BAP and 2.0 mg/l IBA; (e) callus formation of almonds of the M41 Alex variety over the entire surface of the cutting explant on the medium with the addition of 2.5 mg/l BAP and 2.5 mg/l IBA

Figure 6.

In vitro cultivation of Tonda Gentile Romana hazelnut explants during pre-adaptation: (a) coconut; (b) pearlite; (c) a mixture of pearlite and coconut (1:1)
In vitro cultivation of Tonda Gentile Romana hazelnut explants during pre-adaptation: (a) coconut; (b) pearlite; (c) a mixture of pearlite and coconut (1:1)

The height of the shoot of regenerants at different concentrations of BAP for fifth, seventh and 10th passages by the overlay passage method, mm

Variety BAP concentration, mg/l
0.5 1.0 1.5 2.0 2.5
Hazelnut
Trapezund 92/90/90* 98/94/96 91/83/79 71/66/62 61/51/-
Jefferson 100/102/99 105/107/99 98/95/83 89/76/74 70/65/61
Tonda Gentile Romana 98/97/100 96/98/97 93/88/85 73/58/53 59/52/50
Almond
E5 Borozan 76/74/78 99/98/98 98/64/52 51/43/41 36/-/-
M41 Alex 71/73/75 102/103/96 94/61/52 46/38/36 33/-/-
Georgia 69/66/68 97/98/96 84/55/51 41/32/30 30/22/-
Louise 67/66/64 94/92/95 85/82/56 44/33/33 18/-/-

Composition of modified artificial nutrient media

Component Medium composition, mg/l
MSmod* QLmod DKWmod NAMmod NRMmod
NH4NO3 1650.0 400.0 1416.0 900 530
KNO3 1900.0 1800.0 – 250 550
KH2PO4 170.0 270.0 265.0 1550 1300
MgSO4×7H2O 370.0 360.0 740.0 2050 1650
K2SO4 – – 1600.0 – –
Ca(NO3)2×4H2O – 833.8 1365 1050 700
CaCl2×2H2O 440.0 – 150 45 90
FeSO4×7H2O 27.80 33.8 –
Na2MoO4×2H2O 37.3 45.4 –
Ferrilene 4.8 Orto-Orto – 114.63
MnSO4×4H2O 22.3 0.76 33.5 6.0 20.0
H3BO3 6.2 4.80 11.0 6.5
ZnSO4×7H2O 8.6 – 11.0 8,8
Zn(NO3)2 – 17.0 – –
CuSO4×5H2O 0.0250.25 0.25 3.2 2.5
Na2MoO4×2H2O 0.39 0.1 2.5
CoCl×6H2O 0.025 –
KJ 0.83 0.08 –
NiSO4×6H2O – 0.0053 –
BAP 1.5 during introduction and multiplication and 0.3 during rhizogenesis
IBA 0.3 during introduction and multiplication and 1.5 during rhizogenesis
Vitamin B1 1.0
Vitamin B6 0.6
Vitamin PP 1.0
Vitamin C 3.0
Meso-inositol 100.0
Glycine 1.0
Sucrose/glucose Sucrose 30 × 103 almond; glucose 15 × 103 hazelnut

Self-intoxication of primary explants depending on the medium and growing conditions of donor plant varieties, %

Variety Nutrient medium
MSmod QLmod DKWmod NAMmod NRMmod
Hazelnut
Trapezund 100/16* 98/11 83/4 70/2 24/1
Jefferson 98/19 91/12 77/9 63/1 22/0
Tonda Gentile Romana 100/32 98/26 90/11 66/3 29/2
Almond
E5 Borozan 23/12 6/2 21/18 6/1 8/3
M41 Alex 19/12 5/2 12/9 0/0 7/2
Georgia 14/10 3/1 11/9 0/0 4/0
Louise 8/6 - 6/5 0/0 1/0

The height of regenerants on the 90th day of in vitro cultivation on different nutrient media

Variety Nutrient medium
MSmod QLmod DKWmod NAMmod NRMmod
Height of regenerants, mm
Hazelnut
Trapezund 59/23* 63/27 98/79 86/74 116/91
Jefferson 41/16 58/32 108/87 88/75 109/101
Tonda Gentile Romana 79/29 72/35 111/86 99/70 121/97
Almond
E5 Borozan 84/21 93/73 39/12 123/98 97/69
M41 Alex 78/18 84/71 37/13 134/94 96/66
Georgia 71/13 69/63 33/15 103/84 81/61
Louise 45/12 67/64 23/12 101/85 77/63

Peculiarities of the photoautotrophic microclonal propagation of hazelnut plants with different pre-adaptation of the Tonda Gentile Romana variety

Indicator Mediumт
coconut pearlite mixture of pearlite and coconut (1:1)
Germination conditions
in vitro micro greenhouse in vitro micro greenhouse in vitro micro greenhouse
Survival rate, % 58 67 60 69 69 94
Damaged leaf area, % 12 27 15 19 12 14
The height of plants on the 14th day of growing FMP, mm 189 213 162 184 221 267

The height of the progeny shoots with different duration of the dormant state of explant donors, mm

Variety Age of donors, days
0 45 90 120
Hazelnut
Trapezund 99 114 139 131
Jefferson 98 114 154 152
Tonda Gentile Romana 96 107 133 138
Almond
E5 Borozan 95 102 109 110
M41 Alex 91 100 103 101
Georgia 87 93 108 105
Louise 84 98 112 121

Shoot height of regenerants depending on the age of mother plants of explant donors at different periods of passage, mm

Variety Age of donors, days
30 45 90 120 180
Hazelnut
Trapezund 35/24/- 49/41/32 97/84/81 112/109/115 116/114/119
Jefferson 36/31/29 41/32/29 98/95/83 123/127/119 122/127/124
Tonda Gentile Romana 39/30/- 44/35/31 93/89/85 126/119/129 129/133/130
Almond
E5 Borozan 28/-/- 54/52/36 98/84/52 101/111/113 143/148/149
M41 Alex 17/-/- 47/43/26 94/81/52 98/95/103 141/146/145
Georgia -/-/- 36/33/- 84/65/51 89/91/92 103/105/104
Louise 24/-/- 39/37/30 85/72/56 96/99/99 107/109/110

The height of regenerants on different unloading media on the 90th day of propagation, mm

Variety/media Nutrient medium
DKWmod ULM* DKWmod NRMmod ULM NRMmod
Passage
5 6 7/12 5 6 7/12
Hazelnut
Trapezund 79 100 111/77 91 109 133/98
Jefferson 87 106 110/83 101 112 139/104
Tonda Gentile Romana 86 113 122/91 97 108 151/ 99
Variety/media QLmod ULM QLmod NAMmod ULM NAMmod
Passage
5 6 7/10 5 6 7/10
Almond
E5 Borozan 73 106 109/65 98 112 114/95
M41 Alex 71 102 106/54 94 116 109/81
Georgia 63 91 98/57 84 94 103/69
Louise 64 97 101/50 85 90 /94/69

The height of regenerants on the 90th day of propagation with different selection of passages and cytokinin–auxin index, mm

Variety Multiplication Induction of rhizogenesis
Passage
5 6 7/10 5 6 7/10
Cytokinin–auxin index
1.5/0.3 0.3/1.5 1.5/0.3 0.3/1.5 1.5/0.3 0.3/1.5
Hazelnut
Trapezund 91 114 109/90 109 129 130/99
Jefferson 101 121 97/94 117 132 139/103
Tonda Gentile Romana 97 124 111/96 103 121 132/97
Almond
E5 Borozan 98 103 100/91 102 107 103/104
M41 Alex 94 100 97/89 108 112 107/101
Georgia 84 91 89/83 94 99 97/91
Louise 85 93 86/74 96 97 98/90
eISSN:
2199-5907
ISSN:
0071-6677
Langue:
Anglais
Périodicité:
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Sujets de la revue:
Life Sciences, Plant Science, Medicine, Veterinary Medicine
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