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Comparison of bacterial communities in roots of selected trees with and without summer truffle (Tuber aestivum) ectomycorrhiza

Marta Siebyła
Marta Siebyła
 et 
Iwona Szyp-Borowska
Iwona Szyp-Borowska
   | 08 juin 2021
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Article Category: Original article
Publié en ligne: 08 juin 2021
Pages: 97 - 111
Reçu: 30 nov. 2020
Accepté: 30 mars 2021
DOI: https://doi.org/10.2478/ffp-2021-0011
© 2021 Marta Siebyła, Iwona Szyp-Borowska, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Degree of root colonization expressed in % by bacteria, taking into account the research area (G, M, W), the variant (2, 3, 4, 5, 6), the season (autumn, spring) and year of evaluation (2017, 2018) for samples taken from Corylus avellana roots
Degree of root colonization expressed in % by bacteria, taking into account the research area (G, M, W), the variant (2, 3, 4, 5, 6), the season (autumn, spring) and year of evaluation (2017, 2018) for samples taken from Corylus avellana roots

Figure 2

PcoA distance plots: A – Weighted UniFrac and B– unweighted UniFrac
PcoA distance plots: A – Weighted UniFrac and B– unweighted UniFrac

Figure 3

PcoA distance plot – Bray-Curtis dissimilarity
PcoA distance plot – Bray-Curtis dissimilarity

Figure 4

PcoA distance plot – Jaccard index
PcoA distance plot – Jaccard index

Figure 5

Boxplot charts with Bray-Curtis (A), Unweighted (B), Jaccard (C), and Weighted (D) indicators for WC sample
Boxplot charts with Bray-Curtis (A), Unweighted (B), Jaccard (C), and Weighted (D) indicators for WC sample

Percentage of bacteria of the dominant genera by season (S – spring; A – autumn) of the year (2017, 2018)

Genus 2017 2018
S A S A
Bacillus 6.8 6.8 3.4 11.2
Erwinia 9.6 0.0 3.4 3.4
Pseudomonas 20.5 32.9 2.2 14.6
Rahnella 1.4 1.4 30.3 0.0
Serratia 4.1 4.1 22.5 0.0
Other 6.8 5.5 6.7 2.2

Average values of hydrothermal coefficient K and the average sum of precipitation for months IV–X at the nearest measuring stations in Kielce and Cracow in 2016–2018

Station name K Total precipitation by month Years
IV–X I–XII
Kielce 1.44 378.6 619.5 2016
Cracow 1.95 553.6 745.3
Kielce 2.27 548.9 731.8 2017
Cracow 2.11 545.9 702.3
Kielce 0.95 340.1 486.7 2018
Cracow 1.25 459.0 568.7

Percentage of bacteria of dominant genera, indicating variant (T – truffle; C– control) obtained by Sanger sequencing. The numbers in bold indicate the presence of a particular genus of bacteria in a particular sample

Genus 2017 2018
T C T C
Bacillus 6.8 6.8 6.7 7.9
Pseudomonas 32.9 20.5 11.2 3.4
Erwinia 8.2 1.4 5.6 –
Rahnella – 4.1 18.0 11.2
Serratia 6.8 1.4 9.0 5.6
Brevibacterium 1.4 – – –
Enterobacter 1.4 1.4 1.1 –
Viridibacillus 1.4 1.4 – –
Microbacterium – 1.4 – –
Pantoea – 1.4 – –
Bradyrhizobium – – 2.2 –
Acinetobacter – – 1.1 –
Methylobacterium – – – 1.1
Viridibacillus – – – 1.1

Alpha biodiversity index values for Chao, Faith’s, OTUs, Shannon’s, Heip’s and Simpson’s indices for selected samples representing sites W, G and M

Sample Chao Faith’s OTU Shannon’s Heip’s Simpson’s
W1 188.00 13.77 188.00 7.12 0.74 0.99
W2 178.00 13.53 178.00 7.09 0.76 0.99
W4 362.00 14.56 362.00 7.78 0.61 0.99
W5 272.00 13.36 272.00 7.43 0.63 0.99
G1 246.00 13.44 246.00 7.49 0.73 0.99
G2 120.00 12.74 120.00 6.51 0.76 0.99
G3 233.00 15.23 233.00 7.25 0.65 0.99
G4 184.00 12.87 184.00 6.98 0.68 0.99
G5 239.00 13.73 239.00 7.46 0.74 0.99
G6 214.00 13.48 214.00 7.23 0.70 0.99
M1 162.00 12.44 162.00 6.35 0.50 0.98
M2 245.00 15.21 245.00 7.50 0.74 0.99
M3 252.00 14.80 252.00 7.53 0.73 0.99
M4 203.00 13.38 203.00 7.14 0.69 0.99
M5 200.00 13.54 200.00 7.07 0.67 0.99
M6 121.00 11.12 121.00 6.48 0.73 0.98

j.ffp-2021-0011.tab.005

OTU ID (genus) VARIANT
T C
1 2 3
Acidicapsa 0 834
Acidipila 0 1813
Acidisphaera 0 1306
Acidobacterium 0 759
Acidocella 0 35
Acidothermus 0 184
Acidovorax 0 60
Actinoallomurus 0 117
Actinocrinis 0 473
Actinokineospora 0 79
Actinospica 0 717
Actinosynnema 0 206
Afipia 262 0
Agromyces 90 0
Albimonas 29 0
Aliidongia 0 955
Allokutzneria 0 54
Alteromonas 37 0
Amaricoccus 55 0
Angustibacter 0 296
Arcobacter 38 0
Asaia 48 0
Azoarcus 54 0
Azospirillum 0 39
Bacillus 31 0
Baekduia 145 0
Bauldia 25 0
Bdellovibrio 0 32
Blastococcus 76 0
Blastomonas 0 87
Bradyrhizobium 0 153
Brumimicrobium 0 83
Bryobacter 0 297
Bryocella 0 112
Caenimonas 76 0
Caldicellulosiruptor 0 102
Calditerricola 0 14
Catelliglobosispora 0 69
Catenulispora 0 308
Chelativorans 129 0
Chitinophaga 302 0
Chondromyces 22 0
Collimonas 0 41
Conexibacter 0 333
Denitratisoma 71 0
Desulfonatronum 171 0
Desulfuromonas 280 0
Devosia 0 246
Dinghuibacter 0 19
Dokdonella 0 39
Dyadobacter 0 28
Elioraea 0 37
Erythrobacter 91 0
Ferrimicrobium 317 0
Ferruginibacter 213 0
Fibrobacter 45 0
Flavitalea 0 127
Frigoribacterium 0 35
Goodfellowiella 0 151
Granulicella 0 2639
Haliangium 70 0
Hartmannibacter 35 0
Helicobacter 337 0
Herbaspirillum 0 22
Ideonella 47 0
Inquilinus 0 24
Jatrophihabitans 0 175
Jiangella 253 0
Kaistibacter 0 166
Kineosporia 0 126
Kofleria 37 0
Krasilnikovia 246 0
Kutzneria 0 92
Labedaea 0 173
Lentzea 306 0
Leptothrix 0 265
Lewinella 159 0
Lysobacter 0 282
Marmoricola 0 37
Mesorhizobium 256 0
Methylobrevis 66 0
Methyloceanibacter 556 0
Methylosinus 0 103
Methylotenera 2469 0
Microcystis 211 0
Mycobacterium 0 107
Nakamurella 63 0
Natranaerovirga 74 0
Niabella 211 0
Niameybacter 0 108
Niastella 475 0
Nitratifractor 0 173
Nitrosospira 813 0
Nonomuraea 0 65
Occallatibacter 0 249
Paludibaculum 0 2367
Parasegetibacter 194 0
Pedobacter 0 126
Pelomonas 83 0
Phaselicystis 84 0
Phycicoccus 0 31
Phytomonospora 214 0
Piscinibacter 86 0
Plantactinospora 353 0
Polaromonas 57 0
Promicromonospora 138 0
Providencia 55 0
Pseudomonas 0 28
Pseudonocardia 0 379
Pseudoxanthobacter 0 56
Puia 0 508
Reyranella 0 86
Rhizocola 20 0
Rhizomicrobium 0 651
Rhizorhabdus 2505 0
Rhodanobacter 0 75
Rhodoferax 329 0
Rhodomicrobium 375 0
Rhodopila 0 26
Rhodoplanes 148 0
Rhodopseudomonas 0 440
Roseiarcus 0 3935
Rubrivivax 0 49
Saccharomonospora 128 0
Salinispora 90 0
Skermanella 0 165
Solirubrobacter 0 654
Sorangium 30 0
Sphingobacterium 391 0
Sphingomonas 838 0
Stenotrophomonas 0 18
Steroidobacter 0 143
Streptacidiphilus 0 494
Streptomyces 683 0
Sulfurisoma 200 0
Tabrizicola 90 0
Telmatobacter 0 5097
Terracidiphilus 0 448
Terriglobus 0 83
Thermocatellispora 0 85
Uliginosibacterium 51 0
Umezawaea 207 0
Virgisporangium 49 0
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