<abstract xmlns="http://www.w3.org/1999/xhtml">

This work is part of the <italic>exsitu</italic> conservation of <italic>Ruta montana</italic> (L.) L., a spontaneous Rutaceae, known for its many therapeutic properties. The aim is to study, under controlled conditions, the effects of daylight and darkness, temperature, salt, and water stress on the germination of its seeds. Salt stress was induced by different molar concentrations of NaCl (0–200 mM), and water stress was simulated using different concentrations of polyethylene glycol (PEG6000) corresponding to different water potentials (0-1.2 MPa).
The results obtained showed that <italic>R. montana</italic> seeds were viable and could germinate both in daylight and in continuous darkness. Germination was possible at temperatures between 10 and 30 °C, with the maximum final germination percentage (FGP = 81.66%) recorded at 20 °C. At this optimum temperature, FGP decreased significantly when the seeds were placed under salt and water stress (<italic>p</italic>&lt; 0.01).This decrease became more and more significant when the osmotic pressure induced by NaCl increased and when the water potential induced by PEG6000 in the medium decreased. The depressive effect of salt and water stress on FGP was verified by a linear regression analysis, which showed high values of the coefficient of determination (<italic>R</italic>2):0.934 for water stress and 0.890 for salt stress. This effect on the velocity coefficient and latency time was more moderate or lower. The thresholds of tolerance to salinity and water deficit found were 150 mM and −1 MPa, respectively, for which the lowest FGP values were recorded with 16.25% for water stress and 11.10% for salt stress.
</abstract>

This work is part of the exsitu conservation of Ruta montana (L.) L., a spontaneous Rutaceae, known for its many therapeutic properties. The aim is to study, under controlled conditions, the effects of daylight and darkness, temperature, salt, and water stress on the germination of its seeds. Salt stress was induced by different molar concentrations of NaCl (0–200 mM), and water stress was simulated using different concentrations of polyethylene glycol (PEG6000) corresponding to different water potentials (0-1.2 MPa).
The results obtained showed that R. montana seeds were viable and could germinate both in daylight and in continuous darkness. Germination was possible at temperatures between 10 and 30 °C, with the maximum final germination percentage (FGP = 81.66%) recorded at 20 °C. At this optimum temperature, FGP decreased significantly when the seeds were placed under salt and water stress (p< 0.01).This decrease became more and more significant when the osmotic pressure induced by NaCl increased and when the water potential induced by PEG6000 in the medium decreased. The depressive effect of salt and water stress on FGP was verified by a linear regression analysis, which showed high values of the coefficient of determination (R2):0.934 for water stress and 0.890 for salt stress. This effect on the velocity coefficient and latency time was more moderate or lower. The thresholds of tolerance to salinity and water deficit found were 150 mM and −1 MPa, respectively, for which the lowest FGP values were recorded with 16.25% for water stress and 11.10% for salt stress.

Germination Responses of <italic>Ruta montana</italic> (L.) L. Seeds From Tessala Mount (Northwest of Algeria) to Different Controlled Environmental Conditions

Laboratory of Plant Biodiversity: Conservation and Valorization, Faculty of Natural Sciences and Life

Ekológia (Bratislava)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Germination Responses of  (L.) L. Seeds From Tessala Mount (Northwest of Algeria) to Different Controlled Environmental Conditions

This work is part of the exsitu conservation of Ruta montana (L.) L., a spontaneous Rutaceae, known for its many therapeutic properties. The aim is to study,...

Germination Responses of Ruta montana (L.) L. Seeds From Tessala Mount (Northwest of Algeria) to Different Controlled Environmental Conditions

Publié en ligne: 25 déc. 2023

Pages: 333 - 341

Reçu: 31 janv. 2023

Accepté: 18 août 2023

DOI: https://doi.org/10.2478/eko-2023-0037

Mots clés
salt stress, germination, temperature, water stress

© 2023 Abdelkrim Bendahoua et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Germination Responses of Ruta montana (L.) L. Seeds From Tessala Mount (Northwest of Algeria) to Different Controlled Environmental Conditions

Publié en ligne: 25 déc. 2023

Pages: 333 - 341

Reçu: 31 janv. 2023

Accepté: 18 août 2023

DOI: https://doi.org/10.2478/eko-2023-0037

Mots cléssalt stress, germination, temperature, water stress

© 2023 Abdelkrim Bendahoua et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
salt stress, germination, temperature, water stress