Accès libre

Optimization of Lyophilized LAMP and RT-PCR Reaction Mixes for Detection of Tuberculosis

Esra Agel
Esra Agel
 et 
Hasan Sagcan
Hasan Sagcan
   | 21 oct. 2020
The EuroBiotech Journal's Cover Image
À propos de cet article
Article précédent
Article suivant
Citez
Article Category: Research Article
Publié en ligne: 21 oct. 2020
Pages: 230 - 236
DOI: https://doi.org/10.2478/ebtj-2020-0027
Mots clés
© 2020 Esra Agel, Hasan Sagcan, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Results of different cryoprelyophilized mixture. 1) Freshly prepared mixture, 2) (0.5 % PEG 8000 + 2.0 % stachyose) Cryoprotectant mixture, 3) (0.5 % PEG 8000 + 1.0 % stachyose) Cryoprotectant mixture.
Results of different cryoprelyophilized mixture. 1) Freshly prepared mixture, 2) (0.5 % PEG 8000 + 2.0 % stachyose) Cryoprotectant mixture, 3) (0.5 % PEG 8000 + 1.0 % stachyose) Cryoprotectant mixture.

Figure 2

Results of lyophilized RT-PCR (a) and lyophilized LAMP (b) mixture. a: only 3. cryoprotectant mixture has positive result in RT-PCR assay. b: Results of LAMP assay 1) DNA ladder (100 bp, NEB), 2) 1. Cryoprotectant mixture, 3) 2. Cryoprotectant mixture, 4) 3. Cryoprotectant mixture 5) Freshly prepared mixture.
Results of lyophilized RT-PCR (a) and lyophilized LAMP (b) mixture. a: only 3. cryoprotectant mixture has positive result in RT-PCR assay. b: Results of LAMP assay 1) DNA ladder (100 bp, NEB), 2) 1. Cryoprotectant mixture, 3) 2. Cryoprotectant mixture, 4) 3. Cryoprotectant mixture 5) Freshly prepared mixture.

Figure 3

RT-PCR results of thermal stability of lyophilized mixtures
RT-PCR results of thermal stability of lyophilized mixtures

Figure 4

Detection limit results of lyophilized RT-PCR (a) and LAMP (b). a: 1) 105 CFU/ml, 2) 104 CFU/ml, 3) 103 CFU/ml, 4) 102 CFU/ml, 5) 101 CFU/ml, 6) negative control. b: a: 1) 105 CFU/ml, 2) 104 CFU/ml, 3) 103 CFU/ml, 4) 102 CFU/ml, 5) 101 CFU/ml, 6) negative control
Detection limit results of lyophilized RT-PCR (a) and LAMP (b). a: 1) 105 CFU/ml, 2) 104 CFU/ml, 3) 103 CFU/ml, 4) 102 CFU/ml, 5) 101 CFU/ml, 6) negative control. b: a: 1) 105 CFU/ml, 2) 104 CFU/ml, 3) 103 CFU/ml, 4) 102 CFU/ml, 5) 101 CFU/ml, 6) negative control

Figure 5

An image of lyophilized mixture in PCR tubes.
An image of lyophilized mixture in PCR tubes.

Cryoprotectant mixtures and amount of ingredients

The protocol of freeze dryer

eISSN:
2564-615X
Langue:
Anglais
Périodicité:
4 fois par an
Sujets de la revue:
Life Sciences, Genetics, Biotechnology, Bioinformatics, other
RSS Feed de la revue