Identification and Quantitation of Nicotine Polacrilex in Nicotine Pouches and Other Oral Nicotine Delivery Products
| 13 juin 2023
À propos de cet article
Publié en ligne: 13 juin 2023
Pages: 43 - 49
Reçu: 22 janv. 2023
Accepté: 25 mars 2023
DOI: https://doi.org/10.2478/cttr-2023-0006
© 2023 Serban C. Moldoveanu, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Figure 1.
Figure 2.
Figure 3.
Levels of % nicotine polacrilex, % nicotine, and of (nicotine)/(nicotine polacrilex) ratio % in several commercially available nicotine pouches.
| Sample | No. of replicates | Found nicotine polacrilex (%) | RSD% | Found nicotine (%) | Ratio (%) |
|---|---|---|---|---|---|
| Sample 1 | 2 | 7.38 | 0.29 | 0.70 | 9.53 |
| Sample 2 | 4 | 8.59 | 1.24 | 0.80 | 9.30 |
| Sample 3 | 2 | 11.54 | 0.67 | 1.32 | 11.42 |
| Sample 4 | 1 | 11.8 | – | 1.17 | 9.91 |
| Sample 5 | 1 | 5.25 | – | 0.63 | 12.10 |
| Sample 6 | 1 | 5.11 | – | 0.59 | 11.45 |
| Sample 7 | 1 | 6.35 | – | 0.83 | 13.06 |
| Sample 8 | 1 | 3.70 | – | 0.66 | 17.81 |
| Sample 9 | 1 | 4.76 | – | 0.83 | 17.42 |
| Sample 10 | 1 | 6.70 | – | 0.97 | 14.47 |
| Sample 11 | 1 | 8.71 | – | 0.90 | 10.34 |
| Sample 12 | 2 | not detected | – | 1.28 | ∞ |
| Sample 13 | 1 | not detected | – | 2.37 | ∞ |
| Sample 14 | 1 | not detected | – | 1.73 | ∞ |
Levels of % nicotine polacrilex, % nicotine, and of (nicotine)/(nicotine polacrilex) ratio % in several nicotine pouches samples with known composition.
| Sample | No. of replicates | Expected nicotine polacrilex (%) | Found nicotine polacrilex (%) | RSD% (of found) | Expected nicotine (%) (by HPLC) | Found nicotine (%) | Ratio (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 6 | 9.72 | 8.92 | 1.61 | 1.94 | 1.70 | 19.09 |
| Sample 2 | 2 | 5.56 | 5.55 | 0.89 | 1.11 | 1.03 | 18.56 |
| Sample 3 | 3 | 5.56 | 5.13 | 2.34 | 1.11 | 0.98 | 19.10 |
| Sample 4 | 3 | 2.78 | 2.84 | 1.47 | 0.56 | 0.53 | 18.66 |
Parameters for the GC/MS on-line analysis of pyrolysates on a DB-1701 type column.
| Parameter | Description |
|---|---|
| GC column | DB-1701 |
| Column dimensions | 60 m long, 0.25 mm id. |
| Film thickness | 1.0 μm |
| Initial oven temperature | 37 °C |
| Initial time | 4.0 min |
| Oven ramp rate | 2 °C/min |
| Oven final first ramp | 60 °C |
| Final time first ramp | 0 min |
| Oven ramp rate | 5 °C/min |
| Oven final temperature | 280 °C |
| Final time | 20 min |
| Total run time | 75.5 min |
| Inlet temperature | 280 °C |
| Inlet mode | Split |
| Carrier gas | Helium |
| Flow mode | Constant flow |
| Flow rate | 1.1 mL/min |
| Nominal initial pressure | 17.5 psi |
| Split ratio | 70:1 |
| Split flow | 76.0 mL/min |
| GC outlet | MSD |
| Outlet pressure | Vacuum |
| MSD transfer line temperature | 280 °C |
| Ion source temperature | 230 °C |
| Quadrupole temperature | 150 °C |
| MSD EM offset | 250 V |
| MSD solvent delay | 2.0 min |
| MSD acquisition mode | TIC |
| Mass range | 29–550 a.u. |
Levels of % nicotine polacrilex, % nicotine, and of (nicotine)/(nicotine polacrilex) ratio % in a tablet, a lozenge, and two gums.
| Sample | No. of replicates | Found nicotine polacrilex (%) | RSD% | Found nicotine (%) | Ratio (%) |
|---|---|---|---|---|---|
| Tablet | 2 | 10.51 | 0.24 | 0.96 | 9.13 |
| Lozenge | 2 | 4.16 | 0.74 | 0.38 | 9.13 |
| Gum 1 | 2 | not detected | – | 0.12 | ∞ |
| Gum 2 | 2 | 1.84 | 0.27 | 0.22 | 11.96 |