<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Currently, several <italic>in vitro</italic> tests are widely used to measure toxicological properties of mainstream smoke (Neutral Red Uptake Assay, Micronucleus assay, Ames Test). These tests are necessary to assess cytotoxicity, genotoxicity, and mutagenicity, but are time consuming. This is essentially due to the preparation and the handling of cells. It is difficult to use these <italic>in vitro</italic> tests as screening method for product testing and development. For a better assessment of the cytotoxicity of the vapor phase, a rapid cell-free method has been developed. This paper describes a capillary electrophoresis cell-free method, based on the depletion of an anti-oxidant L-gamma-glutamyl-L-cysteinylglycine (GSH), applied to an aliquot of vapor phase phosphate buffered saline (PBS)-trapped cigarette smoke (as recommended for <italic>in vitro</italic> testing). The correlation between this method and the survival/viability test (Neutral Red cytotoxicity) is excellent (coefficient of correlation (r = 0.99)).</p></abstract>

Currently, several in vitro tests are widely used to measure toxicological properties of mainstream smoke (Neutral Red Uptake Assay, Micronucleus assay, Ames Test). These tests are necessary to assess cytotoxicity, genotoxicity, and mutagenicity, but are time consuming. This is essentially due to the preparation and the handling of cells. It is difficult to use these in vitro tests as screening method for product testing and development. For a better assessment of the cytotoxicity of the vapor phase, a rapid cell-free method has been developed. This paper describes a capillary electrophoresis cell-free method, based on the depletion of an anti-oxidant L-gamma-glutamyl-L-cysteinylglycine (GSH), applied to an aliquot of vapor phase phosphate buffered saline (PBS)-trapped cigarette smoke (as recommended for in vitro testing). The correlation between this method and the survival/viability test (Neutral Red cytotoxicity) is excellent (coefficient of correlation (r = 0.99)).

Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke

SEITA, Imperial Tobacco Group, 4, rue Andre Dessaux, 45404 Fleury-les-Aubrais, France

Contributions to Tobacco & Nicotine Research

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Currently, several in vitro tests are widely used to measure toxicological properties of mainstream smoke (Neutral Red Uptake Assay, Micronucleus assay, Ames...

Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke

Publié en ligne: 30 déc. 2014

Pages: 157 - 165

Reçu: 25 nov. 2009

Accepté: 11 nov. 2010

DOI: https://doi.org/10.2478/cttr-2013-0893

© 2011 Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.