<abstract xmlns="http://www.w3.org/1999/xhtml"><p>β-Cryptogein, a proteinaceous elicitor from the phytopathogenic fungus <italic>Phytophthoracryptogea</italic>, is known to induce leaf necrosis in tobacco and non-specific resistance (expressed in the perinecrotic leaf area) against a wide range of tobacco pathogens. To reveal mechanisms underlying the acquired resistance, biochemical changes in leaves of β-cryptogein-elicited tobacco were followed three, five and ten days after elicitation. The activities of peroxidase, β-1,3-glucanase and β-glucosidase, as well as the patterns of acidic pathogenesis-related (PR)-proteins were determined. The protected part (perinecrotic area) and the non-protected part (distant extra-perinecrotic area) of leaves of β-cryptogein-stem treated tobacco (cv. Xanthin.c.) were analyzed. Leaves of water-stem treated tobacco served as controls. It was shown that in the protected leaf part β-cryptogein caused significant metabolic shifts early after elicitation, persisting during the whole period studied. An important increase of peroxidase and β-1,3-glucanase activity was recorded. PR-protein components appeared that were absent in the controls. There were negligible changes in β-glucosidase activity. In the non-protected leaf part late and non-significant changes occurred. Taking into account the antimicrobial, regulatory and structure-modifying properties of the biochemical components studied, it may be admitted that β-cryptogein elicited the development of a hostile environment, i.e. a potential for plant resistance against subsequent pathogen invasion.</p></abstract>

β-Cryptogein, a proteinaceous elicitor from the phytopathogenic fungus Phytophthoracryptogea, is known to induce leaf necrosis in tobacco and non-specific resistance (expressed in the perinecrotic leaf area) against a wide range of tobacco pathogens. To reveal mechanisms underlying the acquired resistance, biochemical changes in leaves of β-cryptogein-elicited tobacco were followed three, five and ten days after elicitation. The activities of peroxidase, β-1,3-glucanase and β-glucosidase, as well as the patterns of acidic pathogenesis-related (PR)-proteins were determined. The protected part (perinecrotic area) and the non-protected part (distant extra-perinecrotic area) of leaves of β-cryptogein-stem treated tobacco (cv. Xanthin.c.) were analyzed. Leaves of water-stem treated tobacco served as controls. It was shown that in the protected leaf part β-cryptogein caused significant metabolic shifts early after elicitation, persisting during the whole period studied. An important increase of peroxidase and β-1,3-glucanase activity was recorded. PR-protein components appeared that were absent in the controls. There were negligible changes in β-glucosidase activity. In the non-protected leaf part late and non-significant changes occurred. Taking into account the antimicrobial, regulatory and structure-modifying properties of the biochemical components studied, it may be admitted that β-cryptogein elicited the development of a hostile environment, i.e. a potential for plant resistance against subsequent pathogen invasion.

Biochemical Changes in β-Cryptogein-Elicited Tobacco: A possible Basis of Acquired Resistance

Institute of Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

INRA, Station de PathologieVegetale BP81, 33883 Villenaved'OrnonCedex, France

Altadis, Institut du Tabac, 24100 Bergerac, France

INRA, Station de Botanique et de PathologieVegetale, 06606 Antibes Cedex, France

Contributions to Tobacco & Nicotine Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

β-Cryptogein, a proteinaceous elicitor from the phytopathogenic fungus Phytophthoracryptogea, is known to induce leaf necrosis in tobacco and non-specific...

Biochemical Changes in β-Cryptogein-Elicited Tobacco: A possible Basis of Acquired Resistance

Publié en ligne: 30 déc. 2014

Pages: 53 - 59

Reçu: 09 juil. 2001

Accepté: 20 janv. 2002

DOI: https://doi.org/10.2478/cttr-2013-0730

© 2014 Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.