<abstract xmlns="http://www.w3.org/1999/xhtml"><p> A method has been developed that permits the qualitative and quantitative determination of a number of chlorinated and phosphate insecticide compounds on tobacco. Extraction with acetonitrile, partitioning into petroleum ether, cleanup on a Florisil column are followed by gas chromatography and determination with the microcoulometric system (halogen) and the thermionic detector (phosphorus). A procedure is presented for the extraction and determination of the thiophosphates and water-soluble metabolites remaining in the acetonitrile-water layer after petroleum ether partitioning. Carbamate insecticides are extracted with methylene chloride, partitioned into water, separated by thin-layer chromatography, identified and estimated by colourimetry. Recoveries of the compounds (diazinon, parathion, Guthion, malathion, dimethoate) from tobacco fortified just prior to extraction ranged from 74 to 104 %; DDVP, Dipterex, Metasystox R 50 to 78 %, aldrin, DDT, lindane, thiodan 88 to 101 %, carbaryl, Unden 75 to 82 %, at levels of 0.5 ppm. The results for the field application of these 14 different insecticides on tobacco are presented. They show that there are nil or negligible residues from 8 organophosphorus and 1 carbamate insecticides (Unden); carbaryl and thiodan are well below the legal tolerances. Only the treatment of tobacco with lindane, DDT and aldrin (soil treatment) results in residues above the limit set </p></abstract>

A method has been developed that permits the qualitative and quantitative determination of a number of chlorinated and phosphate insecticide compounds on tobacco. Extraction with acetonitrile, partitioning into petroleum ether, cleanup on a Florisil column are followed by gas chromatography and determination with the microcoulometric system (halogen) and the thermionic detector (phosphorus). A procedure is presented for the extraction and determination of the thiophosphates and water-soluble metabolites remaining in the acetonitrile-water layer after petroleum ether partitioning. Carbamate insecticides are extracted with methylene chloride, partitioned into water, separated by thin-layer chromatography, identified and estimated by colourimetry. Recoveries of the compounds (diazinon, parathion, Guthion, malathion, dimethoate) from tobacco fortified just prior to extraction ranged from 74 to 104 %; DDVP, Dipterex, Metasystox R 50 to 78 %, aldrin, DDT, lindane, thiodan 88 to 101 %, carbaryl, Unden 75 to 82 %, at levels of 0.5 ppm. The results for the field application of these 14 different insecticides on tobacco are presented. They show that there are nil or negligible residues from 8 organophosphorus and 1 carbamate insecticides (Unden); carbaryl and thiodan are well below the legal tolerances. Only the treatment of tobacco with lindane, DDT and aldrin (soil treatment) results in residues above the limit set

Screening Procedures for Organophosphorus, Organochlorine and Carbamate Pesticide Residues on Tobacco

Department of Research and Development, BAT Cigaretten-Fabriken GmbH, Hamburg, Germany

Contributions to Tobacco & Nicotine Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

A method has been developed that permits the qualitative and quantitative determination of a number of chlorinated and phosphate insecticide compounds on...

Screening Procedures for Organophosphorus, Organochlorine and Carbamate Pesticide Residues on Tobacco

Publié en ligne: 12 juil. 2014

Pages: 207 - 214

DOI: https://doi.org/10.2478/cttr-2013-0239

© Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.