<abstract xmlns="http://www.w3.org/1999/xhtml"><p> A method is presented for the determination of insecticides belonging in the group of chlorinated hydrocarbons (organochlorine insecticides) in tobacco and tobacco smoke. The described analytical procedure allows the rapid measurement of insecticide concentrations down to the detection limit of 0.01 ppm. Tobacco or tobacco smoke condensate is extracted with aqueous acetonitrile. The filtered extract is diluted with water, and the insecticide residues are then extracted with petroleum ether. The concentrated petroleum ether extract is chromatographically purified by passage over a Florisil column and, subsequently, eluted with mixtures of diethyl ether and petroleum ether (6 and 15 % of diethyl ether, respectively). After vacuum sublimation the concentrated eluates are qualitatively and quantitatively analysed by gas chromatography using an electron capture detector and by thin-layer chromatography. On an average, 8 % of chlorinated hydrocarbon insecticides contained in tobacco are shown to be transferred into the mainstream smoke. </p></abstract>

A method is presented for the determination of insecticides belonging in the group of chlorinated hydrocarbons (organochlorine insecticides) in tobacco and tobacco smoke. The described analytical procedure allows the rapid measurement of insecticide concentrations down to the detection limit of 0.01 ppm. Tobacco or tobacco smoke condensate is extracted with aqueous acetonitrile. The filtered extract is diluted with water, and the insecticide residues are then extracted with petroleum ether. The concentrated petroleum ether extract is chromatographically purified by passage over a Florisil column and, subsequently, eluted with mixtures of diethyl ether and petroleum ether (6 and 15 % of diethyl ether, respectively). After vacuum sublimation the concentrated eluates are qualitatively and quantitatively analysed by gas chromatography using an electron capture detector and by thin-layer chromatography. On an average, 8 % of chlorinated hydrocarbon insecticides contained in tobacco are shown to be transferred into the mainstream smoke.

The Quantitative Determination of Insecticides in Tobacco and Tobacco Smoke. 1st Report: The Determination of Organochlorine Insecticides / Methoden zur quantitativen Bestimmung von Insektiziden in Tabak und Tabakrauch. I. Mitteilung: Zur Bestimmung von Organo-Chlor-Insektiziden

BAT Cigaretten-Fabriken GmbH Hamburg, Forschung und Entwicklung, Germany

Contributions to Tobacco & Nicotine Research

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A method is presented for the determination of insecticides belonging in the group of chlorinated hydrocarbons (organochlorine insecticides) in tobacco and...

The Quantitative Determination of Insecticides in Tobacco and Tobacco Smoke. 1st Report: The Determination of Organochlorine Insecticides / Methoden zur quantitativen Bestimmung von Insektiziden in Tabak und Tabakrauch. I. Mitteilung: Zur Bestimmung von Organo-Chlor-Insektiziden

Publié en ligne: 03 juil. 2014

Pages: 182 - 188

DOI: https://doi.org/10.2478/cttr-2013-0183

© Beiträge zur Tabakforschung International

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.