<abstract xmlns="http://www.w3.org/1999/xhtml">
<p>Goonch <italic>Bagarius bagarius</italic> (Hamilton, 1822) is one of the largest and fastest-growing catfish of the Indus River found at Taunsa Barrage, Pakistan. The full-length cDNA of the growth hormone gene (600 bp) was amplified by reverse transcription of mRNA isolated from the pituitary gland of <italic>B. bagarius</italic>. The full-length growth hormone gene encodes a putative polypeptide of 200 amino acids with a molecular mass of 22.58 kDa. The precursor of <italic>B. bagarius</italic> growth hormone (GH) is composed of 22 amino acids as a signal peptide and 178 amino acids of a mature peptide. There were six conserved Cys residues in GH protein (20, 71, 135, 173, 190 and 198) that maintain the structural integrity of this protein. One putative N-glycosylation site was present at the 197<sup>th</sup> amino acid. The total number of positively charged (Arg and Lys) and negatively charged (Asp and Glu) residues is 42 and 31, respectively. The <italic>B. bagarius</italic> GH gene shows more than 90% sequence homology with other catfishes. The mature protein GH gene was expressed in <italic>Escherichia coli</italic> using pET-28 expression vector, and the recombinant protein of 19.5 kDa was detected through SDS-PAGE analysis. This study suggests that cloning and expression of <italic>B. bagarius</italic> GH gene would provide basic information for transgenic studies aimed at a faster growth rate. This recombinant GH may be produced on a large scale to exploit its growth-promoting function in other cultured fishes.</p>
</abstract>

Goonch Bagarius bagarius (Hamilton, 1822) is one of the largest and fastest-growing catfish of the Indus River found at Taunsa Barrage, Pakistan. The full-length cDNA of the growth hormone gene (600 bp) was amplified by reverse transcription of mRNA isolated from the pituitary gland of B. bagarius. The full-length growth hormone gene encodes a putative polypeptide of 200 amino acids with a molecular mass of 22.58 kDa. The precursor of B. bagarius growth hormone (GH) is composed of 22 amino acids as a signal peptide and 178 amino acids of a mature peptide. There were six conserved Cys residues in GH protein (20, 71, 135, 173, 190 and 198) that maintain the structural integrity of this protein. One putative N-glycosylation site was present at the 197th amino acid. The total number of positively charged (Arg and Lys) and negatively charged (Asp and Glu) residues is 42 and 31, respectively. The B. bagarius GH gene shows more than 90% sequence homology with other catfishes. The mature protein GH gene was expressed in Escherichia coli using pET-28 expression vector, and the recombinant protein of 19.5 kDa was detected through SDS-PAGE analysis. This study suggests that cloning and expression of B. bagarius GH gene would provide basic information for transgenic studies aimed at a faster growth rate. This recombinant GH may be produced on a large scale to exploit its growth-promoting function in other cultured fishes.

Cloning, Sequencing and Expression of Goonch <italic>Bagarius bagarius</italic> (Hamilton, 1822) Growth Hormone 

Croatian Journal of Fisheries

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Cloning, Sequencing and Expression of Goonch  (Hamilton, 1822) Growth Hormone 

Goonch Bagarius bagarius (Hamilton, 1822) is one of the largest and fastest-growing catfish of the Indus River found at Taunsa Barrage, Pakistan. The...

Cloning, Sequencing and Expression of Goonch Bagarius bagarius (Hamilton, 1822) Growth Hormone

Publié en ligne: 14 nov. 2023

Pages: 173 - 181

Reçu: 09 mars 2022

Accepté: 27 mai 2022

DOI: https://doi.org/10.2478/cjf-2023-0019

Mots clés
wth hormone, Indus River, Taunsa Barrage, Pakistan

© 2023 Haji Muhammad et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Cloning, Sequencing and Expression of Goonch Bagarius bagarius (Hamilton, 1822) Growth Hormone

Publié en ligne: 14 nov. 2023

Pages: 173 - 181

Reçu: 09 mars 2022

Accepté: 27 mai 2022

DOI: https://doi.org/10.2478/cjf-2023-0019

Mots cléswth hormone, Indus River, Taunsa Barrage, Pakistan

© 2023 Haji Muhammad et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
wth hormone, Indus River, Taunsa Barrage, Pakistan