<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.</p>
</abstract>

The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.

Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

Department of Parasitology and Parasitic Diseases

Department of Pathology, Faculty of Veterinary Medicine

University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Ross University School of Veterinary Medicine Basseterre

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase...

Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

Publié en ligne: 17 juin 2014

Pages: 219 - 222

Reçu: 17 juin 2013

Accepté: 02 juin 2014

DOI: https://doi.org/10.2478/bvip-2014-0032

Mots clés
Candida, identification, PCR-RFLP, Chromatic Candida, test API 20 C AUX system, Romania.

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

Publié en ligne: 17 juin 2014

Pages: 219 - 222

Reçu: 17 juin 2013

Accepté: 02 juin 2014

DOI: https://doi.org/10.2478/bvip-2014-0032

Mots clésCandida, identification, PCR-RFLP, Chromatic Candida, test API 20 C AUX system, Romania.

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Candida, identification, PCR-RFLP, Chromatic Candida, test API 20 C AUX system, Romania.