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The aim of the paper is a spectral study of a mural fresco from Serbesti wooden church, Dambovita county, partially painted, a historical monument from 1828 (or 1804 after some other documents). Spectral techniques as X-ray Fluorescence wavelengths dispersive (WDXRF), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) or Raman spectroscopy, have been used in order to highlight the presence of the degradation processes. Also, the chromatic parameters have been used as additional data in order to observe the discoloring aspect of these mural fresco.