Accès libre

Determination of the Relationship Between DNA Methylation Status of KLOTHO and ARNTL Genes With Hypertension

M Osum
M Osum
, 
O Tosun
O Tosun
, 
H Birtan
H Birtan
 et 
R Kalkan
R Kalkan
   | 12 mars 2024
Balkan Journal of Medical Genetics's Cover Image
À propos de cet article
Article précédent
Article suivant
Citez
Article Category: Original Article
Publié en ligne: 12 mars 2024
Pages: 41 - 50
DOI: https://doi.org/10.2478/bjmg-2023-0024
Mots clés
© 2023 M Osum et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1.

Methylated and unmethylated peaks of ARNTL gene for the patient groups. (The peaks obtained in different Tm degrees for the unmethylated and methylated cases were shown. Methylated ARNTL patient 6, 8, 10, and 12 are shown in black, grey, pink, and upper purple, respectively. Unmethylated ARNTL patient 14, 15, 21, 24, 26, 29, and 30 are shown in down purple, green, light green, blue, turquoise, orange, and dark blue, respectively)
Methylated and unmethylated peaks of ARNTL gene for the patient groups. (The peaks obtained in different Tm degrees for the unmethylated and methylated cases were shown. Methylated ARNTL patient 6, 8, 10, and 12 are shown in black, grey, pink, and upper purple, respectively. Unmethylated ARNTL patient 14, 15, 21, 24, 26, 29, and 30 are shown in down purple, green, light green, blue, turquoise, orange, and dark blue, respectively)

Figure 2.

Unmethylated ARNTL patients. ARNTL unmethylated control is shown in blue, and methylated control is shown in orange. Unmethylated ARNTL patient 33, 49, 67, 13 and 45 are shown in green, turquoise, red, purple, and black, respectively.
Unmethylated ARNTL patients. ARNTL unmethylated control is shown in blue, and methylated control is shown in orange. Unmethylated ARNTL patient 33, 49, 67, 13 and 45 are shown in green, turquoise, red, purple, and black, respectively.

Figure 3.

Methylated KLOTHO patients. KLOTHO unmethylated control is shown in purple, and methylated control is shown in red. Methylated KLOTHO patient 25 and 12 are shown in turquoise and light green, respectively.
Methylated KLOTHO patients. KLOTHO unmethylated control is shown in purple, and methylated control is shown in red. Methylated KLOTHO patient 25 and 12 are shown in turquoise and light green, respectively.

Figure 4.

The association between the methylation status of KLOTHO and hypertension for potassium (K)(ns: not significant, **: p-value <0,05)
The association between the methylation status of KLOTHO and hypertension for potassium (K)(ns: not significant, **: p-value <0,05)

Mean Values of Biochemical Parameters for KLOTHO Gene

Parameter Methylated KLOTHO Unmethylated KLOTHO
Hypertensive subjects Control Subjects Hypertensive subjects Control subjects
Fasting glucose (mg/dL) 129.96 ± 83.08 (54) 97.97 ± 12.43 (38) 117.20 ± 34.03 (15) 98.18 ± 5.23 (11)
Triglyceride (mg/dL) 166.82 ±91.28 (38) 122.22 ± 31.10 (37) 183.93 ±104.06 (14) 132.00±28.43 (11)
Total cholesterol (mg/dL) 195.97 ± 61.63 (36) 170.59 ±43.12 (37) 197.07 ± 49.94 (14) 191.09 ± 42.03 (11)
HDL-C (mg/dL) 45.84 ± 14.31 (37) 52.19 ± 12.72 (37) 46.50 ± 14.34 (14) 49.64 ± 6.83 (11)
LDL-C (mg/dL) 131.92 ± 65.55 (37) 129.62 ± 28.28 (37) 125.71 ± 60.77 (14) 130.27 ± 33.02 (11)
Sodium (Na) (mmol/L) 137.98 ± 4.29 (53) 138.26 ± 7.50 (38) 136.60 ± 12.98 (15) 143.27 ± 8.60 (11)
Potassium (K) (mmol/L) 4.45 ± 0.60 (53) 4.88 ± 1.11 (38) 4.71± 0.75 (15) 5.55 ± 1.20 (11)
Creatinine (Cr) (mg/dL) 1.11± 0.59 (54) 0.66 ± 0.14 (38) 1.06 ± 0.95 (15) 0.65 ± 0.11 (11)
Urea (mg/dL) 28.66 ± 23.10 (53) 15.18 ± 3.40 (38) 21.14 ± 17.82 (14) 20.91 ± 13.40 (11)

The Interaction Between ARNTL and KLOTHO Methylation Status

Subjects (p + c)
Methylation Status of ARNTL+KLOTHO Genes Count % p value
ARNTL Methylated+KLOTHO Methylated 53 46.1% p>0.05
ARNTL Methylated+KLOTHO Unmethylated 14 12.1%
ARNTL Unmethylated+KLOTHO Methylated 38 33.0%
ARNTL Unmethylated+KLOTHO Unmethylated 10 8.8%
Total 115 100%

Mean Values of Biochemical Parameters for ARNTL Gene

Parameter Methylated ARNTL Unmethylated ARNTL
Hypertensive subjects Control Subjects Hypertensive subjects Control subjects
Fasting glucose (mg/dL) 120.00 ± 70.48 (42) 96.67 ± 15.54 (21) 142.65 ± 87.50 (23) 98.14 ± 6.02 (21)
Triglyceride (mg/dL) 177.58 ±98.15 (33) 132.50 ± 31.30 (20) 172.06±91.38 (16) 120.57±28.13 (21)
Total cholesterol (mg/dL) 197.58 ± 59.89 (33) 179.60 ±59.04 (20) 195.07 ± 52.06 (14) 168.14 ± 28.81 (21)
HDL-C (mg/dL) 47.55 ± 15.91 (33) 48.55 ± 8.75 (20) 41.87 ± 10.33 (15) 53.48 ± 12.15 (21)
LDL-C (mg/dL) 128.30 ± 60.90 (33) 134.65 ± 35.32 (20) 136.93 ± 71.65 (15) 128.24 ± 26.19 (21)
Sodium (Na) (mmol/L) 137.38 ± 8.36 (42) 138.67 ± 6.63 (21) 138.00 ±4.57 (22) 141.00 ± 9.11 (21)
Potassium (K) (mmol/L) 4.45 ± 0.70 (42) 5.02 ± 1.05 (21) 4.59± 0.57 (22) 5.11 ± 1.30 (21)
Creatinine (Cr) (mg/dL) 1.00± 0.51 (42) 0.64 ± 0.15 (21) 1.07 ± 0.52 (23) 0.68 ± 0.12 (21)
Urea (mg/dL) 26.78 ± 23.29 (40) 17.95 ± 10.51 (21) 23.22 ± 13.49 (23) 15.76 ± 2.95 (21)

KLOTHO and ARNTL gene methylation in hypertensive and control subjects

Subjects KLOTHO gene ARNTL gene
Methylation Unmethylation Methylation Unmethylation
Count %A Count %B Count %A Count %B
Hypertension 62 62.0% 16 59.3% 46 68.7% 27 56.2%
Control 38 38.0% 11 40.7% 21 31.3% 21 43.8%
Total 100 100% 27 100% 67 100% 48 100%
p Value p>0.05 p>0.05
eISSN:
1311-0160
Langue:
Anglais
Périodicité:
2 fois par an
Sujets de la revue:
Medicine, Basic Medical Science, other
RSS Feed de la revue