<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The phenome of <italic>E-cadherin</italic> gene methylation and the expression of latent membrane protein 1 (<italic>LMP-1</italic>) gene are associated with nasopharyngeal carcinoma (NPC). In order to determine whether cooperative <italic>LMP-1</italic> expression or methylation of <italic>E-cadherin</italic> could serve as the potential molecule biomarker target for diagnosis and therapy of NPC, a case-control study including 93 NPC biopsy samples and 100 non cancerous nasopharyngeal swab samples were examined, as well as the strength of association among them by the quantitative polymerase chain reaction (qPCR) and nested-methylation-specific PCR methods. The significantly higher frequency of <italic>LMP-1</italic> expression and <italic>E-cadherin</italic> methylation in NPC biopsy samples, accounting for 76.34 and 73.12%, respectively, compared to non cancerous samples, accounting for 0.00 and 30.00%, respectively, were observed. The significant correlation between the <italic>LMP-1</italic> expression and <italic>E-cadherin</italic> methylation in NPC samples was reported. In detail, in the stage IV of NPC, in case of <italic>LMP-1</italic>-positive samples, 35 of 37 samples (accounting for 94.60%) were positive for methylation of <italic>E-cadherin</italic>. It was demonstrated that cooperative <italic>LMP-1</italic> expression and <italic>E-cadherin</italic> gene methylation could serve as a molecular biomarker in NPC.</p></abstract>

The phenome of E-cadherin gene methylation and the expression of latent membrane protein 1 (LMP-1) gene are associated with nasopharyngeal carcinoma (NPC). In order to determine whether cooperative LMP-1 expression or methylation of E-cadherin could serve as the potential molecule biomarker target for diagnosis and therapy of NPC, a case-control study including 93 NPC biopsy samples and 100 non cancerous nasopharyngeal swab samples were examined, as well as the strength of association among them by the quantitative polymerase chain reaction (qPCR) and nested-methylation-specific PCR methods. The significantly higher frequency of LMP-1 expression and E-cadherin methylation in NPC biopsy samples, accounting for 76.34 and 73.12%, respectively, compared to non cancerous samples, accounting for 0.00 and 30.00%, respectively, were observed. The significant correlation between the LMP-1 expression and E-cadherin methylation in NPC samples was reported. In detail, in the stage IV of NPC, in case of LMP-1-positive samples, 35 of 37 samples (accounting for 94.60%) were positive for methylation of E-cadherin. It was demonstrated that cooperative LMP-1 expression and E-cadherin gene methylation could serve as a molecular biomarker in NPC.

Simultaneously both expression of <italic>LMP-1</italic> and methylation of <italic>E-cadherin</italic>: Molecular biomarker in stage IV of nasopharyngeal carcinoma patients

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Simultaneously both expression of  and methylation of : Molecular biomarker in stage IV of nasopharyngeal carcinoma patients

The phenome of E-cadherin gene methylation and the expression of latent membrane protein 1 (LMP-1) gene are associated with nasopharyngeal carcinoma (NPC). In...

Simultaneously both expression of LMP-1 and methylation of E-cadherin: Molecular biomarker in stage IV of nasopharyngeal carcinoma patients

Article Category: Original Article

Publié en ligne: 27 juil. 2021

Pages: 57 - 66

DOI: https://doi.org/10.2478/bjmg-2021-0005

Mots clés
Biomarker, Diagnosis, gene, Latent membrane protein-1 () gene, Nasopharyngeal carcinoma (NPC), Therapy

© 2021 Lao TD, Truong PK, Thieu HH, Nguyen DH, Nguyen MT, Le TAH, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Simultaneously both expression of LMP-1 and methylation of E-cadherin: Molecular biomarker in stage IV of nasopharyngeal carcinoma patients

Article Category: Original Article

Publié en ligne: 27 juil. 2021

Pages: 57 - 66

DOI: https://doi.org/10.2478/bjmg-2021-0005

Mots clésBiomarker, Diagnosis, gene, Latent membrane protein-1 () gene, Nasopharyngeal carcinoma (NPC), Therapy

© 2021 Lao TD, Truong PK, Thieu HH, Nguyen DH, Nguyen MT, Le TAH, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Biomarker, Diagnosis, gene, Latent membrane protein-1 () gene, Nasopharyngeal carcinoma (NPC), Therapy