Napoleonaea imperialis rinds were separated from the seeds and extracted via maceration using methanol. The crude methanol extract was partitioned with n-hexane and dichloromethane to yield n-hexane fraction (11.01 g), dichloromethane fraction (35.16 g) and methanol fraction (101.75 g). Dichloromethane fraction was chromatographed and purified to give a compound whose structure was elucidated using one-dimension and two-dimension nuclear magnetic resonance (NMR) experiments. The isolated compound was characterized as glycosylated stigmasterol.
