<abstract xmlns="http://www.w3.org/1999/xhtml">

In Cameroon, cowpea plays an important role in traditional agroecosystems. Genetic variation in wild and cultivated cowpea in Cameroon has not yet been documented. Allozyme markers because of their codominance and polymorphism are useful tools for studying genetic variation and disparity in plant species. The present study was undertaken to elucidate the relationship between wild and cultivated cowpea from Cameroon. Ten enzyme systems encoding nineteen isozyme loci were used on 62 cowpea germplasm (45 wild and 17 cultivated). A total of thirty-two alleles were found. One allele was only found in cultivated samples (<italic>Enp98</italic>). Eight alleles were specific only to wild plant (<italic>Amp298</italic>, <italic>Amp3a103</italic>, <italic>Amp496</italic>, <italic>Fdh104</italic>, <italic>Idh295</italic>, <italic>Pgi392</italic>, <italic>Pgm295</italic> and <italic>Sdh95</italic>). Twenty-three alleles were common to both wild and cultivated accessions. <italic>Amp210</italic>2 (z = −4.633, <italic>p</italic> &lt; 0.001) and <italic>Fle396</italic> (z = −2.858, <italic>p</italic> &lt; 0.010) were significantly more represented in cultivated compared to wild cowpea forms. The mean number of alleles per locus in wild (1.632 alleles/locus) cowpea were significantly higher (t = 2.805, <italic>p</italic> &lt; 0.010) compared to cultivated (1.263 alleles/locus) cowpea. Also, the proportion of polymorphic loci (P = 52.63%) and average Nei’s genetic diversity (He = 0.126) were important in wild, compared to the cultivated plants: P = 26.31% and He = 0.063, respectively. The low level of diversity found in domesticated accessions compared to wild can be attributed to a major genetic bottleneck that probably happened during the domestication process. Cluster analysis revealed by UPGMA dendrogram separated the 62 accessions into three clusters. Although an admixture of both wild and cultivated accessions within the same cluster were found, the dendogram, however, highlighted a visible separation between wild and cultivated cowpea. Wild cowpea with many more private alleles indicates an untouched resource available for future breeding.
</abstract>

In Cameroon, cowpea plays an important role in traditional agroecosystems. Genetic variation in wild and cultivated cowpea in Cameroon has not yet been documented. Allozyme markers because of their codominance and polymorphism are useful tools for studying genetic variation and disparity in plant species. The present study was undertaken to elucidate the relationship between wild and cultivated cowpea from Cameroon. Ten enzyme systems encoding nineteen isozyme loci were used on 62 cowpea germplasm (45 wild and 17 cultivated). A total of thirty-two alleles were found. One allele was only found in cultivated samples (Enp98). Eight alleles were specific only to wild plant (Amp298, Amp3a103, Amp496, Fdh104, Idh295, Pgi392, Pgm295 and Sdh95). Twenty-three alleles were common to both wild and cultivated accessions. Amp2102 (z = −4.633, p < 0.001) and Fle396 (z = −2.858, p < 0.010) were significantly more represented in cultivated compared to wild cowpea forms. The mean number of alleles per locus in wild (1.632 alleles/locus) cowpea were significantly higher (t = 2.805, p < 0.010) compared to cultivated (1.263 alleles/locus) cowpea. Also, the proportion of polymorphic loci (P = 52.63%) and average Nei’s genetic diversity (He = 0.126) were important in wild, compared to the cultivated plants: P = 26.31% and He = 0.063, respectively. The low level of diversity found in domesticated accessions compared to wild can be attributed to a major genetic bottleneck that probably happened during the domestication process. Cluster analysis revealed by UPGMA dendrogram separated the 62 accessions into three clusters. Although an admixture of both wild and cultivated accessions within the same cluster were found, the dendogram, however, highlighted a visible separation between wild and cultivated cowpea. Wild cowpea with many more private alleles indicates an untouched resource available for future breeding.

Genetic diversity and relationship between wild and cultivated cowpea [<italic>Vigna unguiculata</italic> (L.) Walp.] as assessed by allozyme markers

Genetics, Biotechnology, Agriculture and Plant Physiology Research Unit, Department of Crop Sciences, Faculty of Agronomy and Agricultural Sciences

Agricultura Tropica et Subtropica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Genetic diversity and relationship between wild and cultivated cowpea [ (L.) Walp.] as assessed by allozyme markers

In Cameroon, cowpea plays an important role in traditional agroecosystems. Genetic variation in wild and cultivated cowpea in Cameroon has not yet been...

Genetic diversity and relationship between wild and cultivated cowpea [Vigna unguiculata (L.) Walp.] as assessed by allozyme markers

Publié en ligne: 17 déc. 2021

Pages: 201 - 208

Reçu: 01 avr. 2021

Accepté: 30 nov. 2021

DOI: https://doi.org/10.2478/ats-2021-0021

Mots clés
allozyme markers, Cameroon, genetic diversity, landrace, wild

© 2021 Eric Bertrand Kouam et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Genetic diversity and relationship between wild and cultivated cowpea [Vigna unguiculata (L.) Walp.] as assessed by allozyme markers

Publié en ligne: 17 déc. 2021

Pages: 201 - 208

Reçu: 01 avr. 2021

Accepté: 30 nov. 2021

DOI: https://doi.org/10.2478/ats-2021-0021

Mots clésallozyme markers, Cameroon, genetic diversity, landrace, wild

© 2021 Eric Bertrand Kouam et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
allozyme markers, Cameroon, genetic diversity, landrace, wild