<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Rickettsia</italic> species are important emerging pathogens causing rickettsial diseases, which are important cause death worldwide. The number of recombinant proteins used for diagnostic and therapeutic applications has increased dramatically, which is important in determination of protein function, structure and antigensity. Although <italic>E. coli</italic> is widely used expression system, the codon bias can hamper protein expression due to the presence of rare codons in gene sequence coding protein of interest. Using bioinformatics tools, rare codon analysis of rickettsial genes was performed and compared to not expressed proteins in both <italic>R. prowazekii</italic> and <italic>R. rickettsii.</italic> A negative correlation between frequencies of rare codons in <italic>Rickettsia</italic> and success of rickettsial protein expression was observed. This study suggested a useful tool to improve rickettsial recombinant protein expression in <italic>E. coli</italic>.</p>
</abstract>

Rickettsia species are important emerging pathogens causing rickettsial diseases, which are important cause death worldwide. The number of recombinant proteins used for diagnostic and therapeutic applications has increased dramatically, which is important in determination of protein function, structure and antigensity. Although E. coli is widely used expression system, the codon bias can hamper protein expression due to the presence of rare codons in gene sequence coding protein of interest. Using bioinformatics tools, rare codon analysis of rickettsial genes was performed and compared to not expressed proteins in both R. prowazekii and R. rickettsii. A negative correlation between frequencies of rare codons in Rickettsia and success of rickettsial protein expression was observed. This study suggested a useful tool to improve rickettsial recombinant protein expression in E. coli.

Rare Codon Analysis in <italic>Rickettsia</italic> Affecting Recombinant Protein Expression in <italic>Escherichia coli</italic>

University Center Belhadj Bouchaib of Ain-Temouchent

Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition

Banat’s University of Agriculture Science and Veterinary Medicine “King Michael I of Romania” fromTimisoara

Advanced Research in Life Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Rare Codon Analysis in  Affecting Recombinant Protein Expression in 

Rickettsia species are important emerging pathogens causing rickettsial diseases, which are important cause death worldwide. The number of recombinant proteins...

Rare Codon Analysis in Rickettsia Affecting Recombinant Protein Expression in Escherichia coli

Publié en ligne: 01 oct. 2020

Pages: 30 - 35

Reçu: 01 juin 2020

Accepté: 01 sept. 2020

DOI: https://doi.org/10.2478/arls-2020-0015

Mots clés
rare codon analysis, codon bias, protein expression,

© 2020 Mahfoud Bakli et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Rare Codon Analysis in Rickettsia Affecting Recombinant Protein Expression in Escherichia coli

Publié en ligne: 01 oct. 2020

Pages: 30 - 35

Reçu: 01 juin 2020

Accepté: 01 sept. 2020

DOI: https://doi.org/10.2478/arls-2020-0015

Mots clésrare codon analysis, codon bias, protein expression,

© 2020 Mahfoud Bakli et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
rare codon analysis, codon bias, protein expression,