<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The search is still on for the molecular processes associated with the development and metabolism of skeletal muscles. Selection conducted in farm animals is focused on high muscle mass because it delivers higher economic profit. The present study aimed to shed light on mRNA degradome signals that could be characteristic for molecular processes associated with an abundance of muscle mass and to identify miRNA regulatory networks controlling these processes in pigs applying next-generation-sequencing (NGS). In the study, over 10,000 degraded transcripts were identified per sample, with the highest abundance for genes encoding mitochondrial proteins (<italic>COXs</italic>, <italic>NDs</italic>, <italic>CYTB</italic>, <italic>ATP6</italic> and <italic>ATP8</italic>). Moreover, only 26% of the miRNA targets were found within this degraded transcript pool, which suggested for miRNAs other molecular mechanism at different level of gene expression than mRNA degradation. On the other hand, a small share of the identified degraded transcripts associated with miRNA regulation suggests a different mechanism of mRNA degradation for identified degraded transcropts. Subsequently, most of the miRNA gene degraded targets, such as <italic>ENO3</italic>, <italic>CKM</italic>, <italic>CRYAB</italic> and <italic>ADAM19</italic> encode proteins involved in the muscle mass control. The present study showed an interesting dependence between miRNAs and their targets. Nevertheless, the complete view of the miRNA regulatory network could be a subject of further advanced research, which would employ a miRNA transfection procedure in skeletal muscle cell cultures.</p></abstract>

The search is still on for the molecular processes associated with the development and metabolism of skeletal muscles. Selection conducted in farm animals is focused on high muscle mass because it delivers higher economic profit. The present study aimed to shed light on mRNA degradome signals that could be characteristic for molecular processes associated with an abundance of muscle mass and to identify miRNA regulatory networks controlling these processes in pigs applying next-generation-sequencing (NGS). In the study, over 10,000 degraded transcripts were identified per sample, with the highest abundance for genes encoding mitochondrial proteins (COXs, NDs, CYTB, ATP6 and ATP8). Moreover, only 26% of the miRNA targets were found within this degraded transcript pool, which suggested for miRNAs other molecular mechanism at different level of gene expression than mRNA degradation. On the other hand, a small share of the identified degraded transcripts associated with miRNA regulation suggests a different mechanism of mRNA degradation for identified degraded transcropts. Subsequently, most of the miRNA gene degraded targets, such as ENO3, CKM, CRYAB and ADAM19 encode proteins involved in the muscle mass control. The present study showed an interesting dependence between miRNAs and their targets. Nevertheless, the complete view of the miRNA regulatory network could be a subject of further advanced research, which would employ a miRNA transfection procedure in skeletal muscle cell cultures.

Identification of mRNA Degradome Variation Dependent on Divergent Muscle Mass in Different Pig Breeds

University Centre of Veterinary Medicine, University of Agriculture in Krakow

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

The search is still on for the molecular processes associated with the development and metabolism of skeletal muscles. Selection conducted in farm animals is...

Identification of mRNA Degradome Variation Dependent on Divergent Muscle Mass in Different Pig Breeds

Publié en ligne: 07 nov. 2020

Pages: 1241 - 1256

Reçu: 21 janv. 2020

Accepté: 25 juin 2020

DOI: https://doi.org/10.2478/aoas-2020-0065

Mots clés
degradome, miRNA, skeletal muscle, muscle fibre

© 2020 Katarzyna L. Piórkowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Identification of mRNA Degradome Variation Dependent on Divergent Muscle Mass in Different Pig Breeds

Publié en ligne: 07 nov. 2020

Pages: 1241 - 1256

Reçu: 21 janv. 2020

Accepté: 25 juin 2020

DOI: https://doi.org/10.2478/aoas-2020-0065

Mots clésdegradome, miRNA, skeletal muscle, muscle fibre

© 2020 Katarzyna L. Piórkowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Mots clés
degradome, miRNA, skeletal muscle, muscle fibre