<abstract xmlns="http://www.w3.org/1999/xhtml"><p>µ-calpain (<italic>CAPN1</italic>) and calpastatin (<italic>CAST</italic>) genes play key roles in protein turnover. The present study aimed to identify the variants in these genes associated with growth and ultrasound carcass traits in Santa Inês sheep. A sample of 192 no full sibling Santa Inês lambs was used. Fragments of the <italic>CAST</italic> and <italic>CAPN1</italic> genes were amplified and next-generation sequencing was performed in the MiSeq platform. Variants in the <italic>CAPN1</italic> and <italic>CAST</italic> sequences were then detected using bioinformatic tools. Withers and croup heights, body length, thoracic and croup widths, thoracic and leg girths, body depth, carcass fat score, rib eye area, fat thickness, body weights were recorded at weaning and at 140 days post-weaning, and average daily gain post-weaning was calculated. Both single-locus and haplotype association analyses were performed with the model as follows: farm (2 levels), year (4 levels), the month of birth (12 levels), and the covariate age of the animal. The fragments amplified included 4,514 bp between the 20th and 23rd exons of <italic>CAST</italic> as well as 3,927 bp between the 12th and 21st exons of <italic>CAPN1</italic>. In these regions, 58 (<italic>CAST</italic>) and 45 (<italic>CAPN1</italic>) variants were identified. In the <italic>CAST</italic> gene, the single-locus analysis revealed 22 suggestive additive effects (P&lt;0.05) on several growth and carcass traits. Moreover, haplotype substitutions were associated with rib eye area (–0.689±0.290), average daily gain (–23.6±10.4), thoracic girth (–2.72±1.27), body length (–3.38±1.49), and leg girth (–2.84±1.37). Regarding the <italic>CAPN1</italic> gene, the single-locus analysis identified seven suggestive additive effects, while only one haplotype replacement effect on fat thickness (–0.0143±0.0053) was detected. The results of the present study suggest that variants in the <italic>CAPN1</italic> and <italic>CAST</italic> genes are associated with growth and ultrasound carcass traits in Santa Inês sheep, which may be a source of information to improve knowledge regarding the genetic control of these traits.</p></abstract>

µ-calpain (CAPN1) and calpastatin (CAST) genes play key roles in protein turnover. The present study aimed to identify the variants in these genes associated with growth and ultrasound carcass traits in Santa Inês sheep. A sample of 192 no full sibling Santa Inês lambs was used. Fragments of the CAST and CAPN1 genes were amplified and next-generation sequencing was performed in the MiSeq platform. Variants in the CAPN1 and CAST sequences were then detected using bioinformatic tools. Withers and croup heights, body length, thoracic and croup widths, thoracic and leg girths, body depth, carcass fat score, rib eye area, fat thickness, body weights were recorded at weaning and at 140 days post-weaning, and average daily gain post-weaning was calculated. Both single-locus and haplotype association analyses were performed with the model as follows: farm (2 levels), year (4 levels), the month of birth (12 levels), and the covariate age of the animal. The fragments amplified included 4,514 bp between the 20th and 23rd exons of CAST as well as 3,927 bp between the 12th and 21st exons of CAPN1. In these regions, 58 (CAST) and 45 (CAPN1) variants were identified. In the CAST gene, the single-locus analysis revealed 22 suggestive additive effects (P<0.05) on several growth and carcass traits. Moreover, haplotype substitutions were associated with rib eye area (–0.689±0.290), average daily gain (–23.6±10.4), thoracic girth (–2.72±1.27), body length (–3.38±1.49), and leg girth (–2.84±1.37). Regarding the CAPN1 gene, the single-locus analysis identified seven suggestive additive effects, while only one haplotype replacement effect on fat thickness (–0.0143±0.0053) was detected. The results of the present study suggest that variants in the CAPN1 and CAST genes are associated with growth and ultrasound carcass traits in Santa Inês sheep, which may be a source of information to improve knowledge regarding the genetic control of these traits.

Single Loci and Haplotypes in <italic>CAPN1</italic> and <italic>CAST</italic> Genes are Associated with Growth, Biometrics, and <italic>in Vivo</italic> Carcass Traits in Santa Inês Sheep

State University of Ponta Grossa – 4748

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Single Loci and Haplotypes in  and  Genes are Associated with Growth, Biometrics, and  Carcass Traits in Santa Inês Sheep

µ-calpain (CAPN1) and calpastatin (CAST) genes play key roles in protein turnover. The present study aimed to identify the variants in these genes associated...

Single Loci and Haplotypes in CAPN1 and CAST Genes are Associated with Growth, Biometrics, and in Vivo Carcass Traits in Santa Inês Sheep

Publié en ligne: 04 mai 2020

Pages: 465 - 483

Reçu: 04 juin 2019

Accepté: 13 déc. 2019

DOI: https://doi.org/10.2478/aoas-2020-0007

Mots clés
body weight, fat, lambs, muscle, selection

© 2020 Alessandro Lima Machado et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Single Loci and Haplotypes in CAPN1 and CAST Genes are Associated with Growth, Biometrics, and in Vivo Carcass Traits in Santa Inês Sheep

Publié en ligne: 04 mai 2020

Pages: 465 - 483

Reçu: 04 juin 2019

Accepté: 13 déc. 2019

DOI: https://doi.org/10.2478/aoas-2020-0007

Mots clésbody weight, fat, lambs, muscle, selection

© 2020 Alessandro Lima Machado et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
body weight, fat, lambs, muscle, selection