<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The use of a non-invasive approach to collect biological samples from natural populations represents a great means of gathering information while avoiding handling animals. Even if corticosterone is the main glucocorticoid investigated in birds, there has been observed a proportional direct link between corticosterone and cortisol concentrations. Dehydroepiandrosterone (DHEA) can be produced by the adrenal cortex and should have prominent antiglucocorticoid properties also in birds. The aim of this study was to verify if there is any difference in the cortisol and DHEA feather concentrations between clinically normal and physiologically compromised Griffon vultures (<italic>Gyps fulvus</italic>) through the non-invasive approach of collecting moulted feathers without having to pluck them from the bird. The study was carried out using 8 physiologically compromised (PC) Griffons and 9 clinically normal Griffons considered as the control (CTRL) group that were necropsied or from the wildlife rehabilitation centre. Primary and secondary covert feathers were either collected directly from the birds’ cage floors, or, in the case of dead Griffons, they were plucked off the animals. The results, obtained by RIA, revealed that both cortisol (P&lt;0.01) and DHEA (P&lt;0.05) feather concentrations were higher in the PC than in the CTRL group. No difference was observed by comparing the cortisol/DHEA ratio between the two evaluated groups (P=0.15). Pearson’s correlation coefficients showed no correlation between feather hormone concentrations in the PC group (r=0.01, P=0.96) while a positive correlation in the CTRL group (r=0.65, P=0.006) was observed. In conclusion, our study reveals that moulted feathers can be a non-invasive and an interesting tool to evaluate the allostatic load of wild birds and they allowed better understanding the relationship between hormones of the hypothalamic–pituitary–adrenal axis and the physiological status of the birds.</p></abstract>

The use of a non-invasive approach to collect biological samples from natural populations represents a great means of gathering information while avoiding handling animals. Even if corticosterone is the main glucocorticoid investigated in birds, there has been observed a proportional direct link between corticosterone and cortisol concentrations. Dehydroepiandrosterone (DHEA) can be produced by the adrenal cortex and should have prominent antiglucocorticoid properties also in birds. The aim of this study was to verify if there is any difference in the cortisol and DHEA feather concentrations between clinically normal and physiologically compromised Griffon vultures (Gyps fulvus) through the non-invasive approach of collecting moulted feathers without having to pluck them from the bird. The study was carried out using 8 physiologically compromised (PC) Griffons and 9 clinically normal Griffons considered as the control (CTRL) group that were necropsied or from the wildlife rehabilitation centre. Primary and secondary covert feathers were either collected directly from the birds’ cage floors, or, in the case of dead Griffons, they were plucked off the animals. The results, obtained by RIA, revealed that both cortisol (P<0.01) and DHEA (P<0.05) feather concentrations were higher in the PC than in the CTRL group. No difference was observed by comparing the cortisol/DHEA ratio between the two evaluated groups (P=0.15). Pearson’s correlation coefficients showed no correlation between feather hormone concentrations in the PC group (r=0.01, P=0.96) while a positive correlation in the CTRL group (r=0.65, P=0.006) was observed. In conclusion, our study reveals that moulted feathers can be a non-invasive and an interesting tool to evaluate the allostatic load of wild birds and they allowed better understanding the relationship between hormones of the hypothalamic–pituitary–adrenal axis and the physiological status of the birds.

Assessment of Cortisol and DHEA Concentrations in Griffon Vulture (<italic>Gyps fulvus</italic>) Feathers to Evaluate its Allostatic Load

Department of Agricultural Food Environmental and Animal Sciences

FoReSTAS Regional Forest Agency for Land and Environment of Sardinia

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Assessment of Cortisol and DHEA Concentrations in Griffon Vulture () Feathers to Evaluate its Allostatic Load

The use of a non-invasive approach to collect biological samples from natural populations represents a great means of gathering information while avoiding...

Assessment of Cortisol and DHEA Concentrations in Griffon Vulture (Gyps fulvus) Feathers to Evaluate its Allostatic Load

Publié en ligne: 28 janv. 2020

Pages: 85 - 96

Reçu: 08 mars 2019

Accepté: 02 août 2019

DOI: https://doi.org/10.2478/aoas-2019-0051

Mots clés
wild birds, raptors, HPA axis, steroids, coverts

© 2020 Gian Nicola Frongia et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Assessment of Cortisol and DHEA Concentrations in Griffon Vulture (Gyps fulvus) Feathers to Evaluate its Allostatic Load

Publié en ligne: 28 janv. 2020

Pages: 85 - 96

Reçu: 08 mars 2019

Accepté: 02 août 2019

DOI: https://doi.org/10.2478/aoas-2019-0051

Mots cléswild birds, raptors, HPA axis, steroids, coverts

© 2020 Gian Nicola Frongia et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
wild birds, raptors, HPA axis, steroids, coverts