Protein | RA | OA | ||||||
---|---|---|---|---|---|---|---|---|
Median | Average | Min. | Max. | Median | Average | Min. | Max | |
PEPT2 | 8 | 8.13 | 4 | 12 | 6 | 5.25 | 0 | 9 |
RFC1 | 8 | 7.93 | 4 | 12 | 6 | 5.63 | 1 | 12 |
MATE1 | 6 | 6.43 | 2 | 9 | 6 | 5.85 | 0 | 12 |
SM (mean ± SD) | HFP (mean ± SD) | |||
---|---|---|---|---|
SLC0C1 | ∆∆CT | 0.437 ± 0.37 | 1.332 ± 0.90 | 0.088 |
SLC02B1 | 31.462 ± 63.66 | 41.233 ± 43.92 | 0.070 | |
SLC04A1 | 14.388 ± 13.31 | 35.838 ± 21.45 | ||
SLC2A9 | 6.197 ± 1.22 | 10.295 ± 8.95 | 0.895 | |
SLC7A5 | 13.45 ± 20.90 | 31.441 ± 28.92 | 0.239 | |
SLC7A6 | 18.971 ± 15.31 | 121.553 ± 125.98 | ||
SLC15A2 | 0.963 ± 1.05 | 2.6 ± 2.47 | ||
SLC16A3 | 154.528 ± 206.39 | 42.223 ± 30.48 | ||
SLC19A1 | 7.35 ± 9.30 | 22.44 ± 15.00 | ||
SLC22A1 | 1.289 ± 2.28 | 1.101 ± 0.89 | 0.307 | |
SLC22A3 | 0.491 ± 0.69 | 2.386 ± 1.84 | ||
SLC22A4 | 2.081 ± 1.72 | 4.371 ± 2.93 | 0.064 | |
SLC22A5 | 1.569 ± 1.49 | 6.656 ± 3.96 | ||
SLC22A18 | 22.775 ± 16.89 | 8.694 ± 2.87 | ||
SLC33A1 | 9.382 ± 5.01 | 25.173 ± 9.62 | ||
SLC47A1 | 2.632 ± 2.84 | 4.3612 ± 2.18 | 0.225 | |
SLC51A | 0.146 ± 0.17 | 0.397 ± 0.12 | 0.275 |
CRP | WBC | Age (years) | Disease duration | DAS28 | ||
---|---|---|---|---|---|---|
SLC15A2 | −0.267 | 0.517 | −0.385 | 0.017 | −0.217 | |
0.488 | 0.154 | 0.306 | 0.965 | 0.576 | ||
SLC22A18 | 0.188 | −0.200 | 0.261 | −0.118 | 0.176 | |
0.603 | 0.580 | 0.466 | 0.746 | 0.627 | ||
SLC16A3 | 0.006 | −0.091 | −0.176 | 0.285 | −0.018 | |
0.987 | 0.803 | 0.626 | 0.425 | 0.960 | ||
SLC33A1 | 0.261 | −0.382 | 0.122 | 0.415 | 0.188 | |
0.467 | 0.276 | 0.738 | 0.233 | 0.603 | ||
SLC19A1 | −0.357 | 0.548 | 0.190 | 0.400 | −0.333 | |
0.385 | 0.160 | 0.651 | 0.326 | 0.420 | ||
SLC47A1 | −0.321 | 0.214 | −0.500 | 0.436 | −0.250 | |
0.482 | 0.645 | 0.253 | 0.328 | 0.589 | ||
SLC2A9 | 0.100 | −0.393 | −0.339 | − |
||
0.798 | 0.295 | 0.372 | ||||
SLC51A | 1.000 | 0.500 | −0.500 | −0.866 | 1.000 | |
− | 0.667 | 0.667 | 0.333 | − | ||
SLC22A1 | −0.238 | 0.595 | −0.132 | 0.146 | −0.238 | |
0.570 | 0.120 | 0.756 | 0.729 | 0.570 | ||
SLC22A3 | −0.464 | 0.429 | 0.214 | 0.436 | −0.429 | |
0.294 | 0.337 | 0.645 | 0.328 | 0.337 | ||
SLC7A5 | −0.050 | 0.333 | −0.519 | 0.373 | −0.033 | |
0.898 | 0.381 | 0.152 | 0.323 | 0.932 | ||
SLC22A4 | 0.036 | 0.036 | −0.286 | 0.036 | ||
0.939 | 0.939 | 0.535 | 0.939 | |||
SLC7A6 | 0.200 | 0.455 | 0.195 | 0.415 | 0.212 | |
0.580 | 0.187 | 0.590 | 0.233 | 0.556 | ||
SLC22A5 | −0.350 | −0.033 | 0.259 | 0.602 | −0.333 | |
0.356 | 0.932 | 0.500 | 0.086 | 0.381 | ||
SLC01C1 | −0.700 | −0.200 | 0.600 | 0.500 | −0.700 | |
0.188 | 0.747 | 0.285 | 0.391 | 0.188 | ||
SLC02B1 | −0.491 | 0.503 | 0.255 | 0.223 | −0.503 | |
0.150 | 0.138 | 0.476 | 0.536 | 0.138 | ||
SLC04A1 | 0.143 | 0.095 | −0.048 | 0.424 | 0.024 | |
0.736 | 0.823 | 0.911 | 0.295 | 0.955 |
CRP | WBC | Age (years) | Disease duration | DAS28 | ||
---|---|---|---|---|---|---|
SLC15A2 | 0.256 | 0.250 | −0.357 | 0.382 | 0.255 | |
0.579 | 0.589 | 0.432 | 0.398 | 0.582 | ||
SLC22A18 | −0.106 | −0.261 | 0.292 | 0.344 | 0.085 | |
0.770 | 0.467 | 0.413 | 0.331 | 0.815 | ||
SLC16A3 | 0.400 | 0.552 | −0.195 | 0.368 | 0.530 | |
0.252 | 0.098 | 0.590 | 0.295 | 0.115 | ||
SLC33A1 | −0.052 | 0.017 | 0.393 | 0.286 | ||
0.894 | 0.966 | 0.295 | 0.456 | |||
SLC19A1 | −0.355 | −0.024 | 0.357 | 0.194 | 0.217 | |
0.388 | 0.955 | 0.385 | 0.645 | 0.606 | ||
SLC47A1 | 0.473 | −0.643 | 0.273 | 0.691 | ||
0.284 | 0.119 | 0.554 | 0.086 | |||
SLC2A9 | −0.279 | −0.283 | 0.117 | −0.177 | 0.185 | |
0.468 | 0.460 | 0.765 | 0.648 | 0.634 | ||
SLC51A | 0.500 | 1.000 | −0.500 | 0.866 | 1.000 | |
0.667 | − | 0.667 | 0.333 | − | ||
SLC22A1 | −0.866 | −0.500 | 0.500 | −0.866 | −0.866 | |
0.333 | 0.667 | 0.667 | 0.333 | 0.333 | ||
SLC22A3 | −0.540 | − |
0.203 | −0.445 | ||
0.134 | 0.601 | 0.230 | ||||
SLC7A5 | 0.516 | 0.486 | 0.200 | 0.088 | 0.530 | |
0.295 | 0.329 | 0.704 | 0.868 | 0.280 | ||
SLC22A4 | −0.185 | −0.357 | 0.327 | −0.182 | ||
0.691 | 0.432 | 0.474 | 0.696 | |||
SLC7A6 | −0.256 | −0.188 | 0.213 | 0.491 | 0.110 | |
0.475 | 0.603 | 0.555 | 0.150 | 0.763 | ||
SLC22A5 | − |
−0.224 | 0.018 | −0.153 | −0.189 | |
0.533 | 0.960 | 0.672 | 0.601 | |||
SLC01C1 | − |
−0.250 | 0.393 | 0.255 | −0.436 | |
0.589 | 0.383 | 0.582 | 0.328 | |||
SLC02B1 | −0.344 | −0.030 | −0.188 | 0.436 | −0.268 | |
0.331 | 0.934 | 0.602 | 0.208 | 0.454 | ||
SLC04A1 | 0.143 | −0.119 | −0.590 | 0.395 | ||
0.736 | 0.779 | 0.123 | 0.333 |
Antibody | Host | Manufacturer | Rate of dilution | Time of incubation/temperature |
---|---|---|---|---|
Anti-PEPT2 (SLC15A2) | Rabbit | Abcam, Cambridge, United Kingdom | 1:75 | 1 h/RT |
Anti-RFC1 (SLC19A1) | Mouse | Santa Cruz Biotechnology Inc., Dallas, TX, USA | 1:100 | 1 h/RT |
Anti-MATE1 (SLC47A1) | Rabbit | Sigma Prestige, Merck, Darmstadt, Germany | 1:200 | 1 h/RT |
SLC transporters | Housekeeping genes | ||
---|---|---|---|
Gene symbol | TaqMan assay ID | Gene symbol | TaqMan assay ID |
Hs00366488_m1 | Hs99999908_m1 | ||
Hs00272374_m1 | Hs02339424_g1 | ||
Hs00351987_m1 | |||
Hs00213714_m1 | |||
Hs01030353_m1 | |||
Hs00983988_m1 | |||
Hs00698884_m1 | |||
Hs00161820_m1 | |||
Hs01001557_m1 | |||
Hs00192639_m1 | |||
Hs01113665_m1 | |||
Hs00358829_m1 | |||
Hs00953344_m1 | |||
Hs00427552_m1 | |||
Hs00945829_m1 | |||
Hs00375985_m1 | |||
Hs00180039_m1 | |||
Hs01010726_m1 | |||
Hs00222691_m1 | |||
Hs01548718_m1 | |||
Hs00929869_m1 | |||
Hs00537914_m1 | |||
Hs00198527_m1 | |||
Hs00188599_m1 | |||
Hs00375768_m1 | |||
Hs00984403_m1 | |||
Hs00188407_m1 | |||
Hs00417125_m1 | |||
Hs00270469_m1 | |||
Hs00217320_m1 | |||
Hs00945650_m1 | |||
Hs00380895_m1 | |||
Hs01057182_m1 | |||
Hs01001183_m1 | |||
Hs00187727_m1 |
Protein | RA | OA | ||||||
---|---|---|---|---|---|---|---|---|
Median | Average | Min. | Max. | Median | Average | Min. | Max. | |
PEPT2 | 8 | 9.47 | 4 | 12 | 8 | 7.17 | 2 | 12 |
RFC1 | 12 | 9.06 | 1 | 12 | 4 | 4.93 | 0 | 12 |
MATE1 | 8 | 7.47 | 2 | 12 | 8 | 6.5 | 1 | 12 |