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Ortho-substituted PCB 153: effects in CHO-K1 cells

Marina Miletić

Marina Miletić

University of Zagreb Faculty of Food Technology and Biotechnology, Laboratory for ToxicologyZagreb, Croatia
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Miletić, Marina
, 
Teuta Murati

Teuta Murati

University of Zagreb Faculty of Food Technology and Biotechnology, Laboratory for ToxicologyZagreb, Croatia
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Murati, Teuta
, 
Branimir Šimić

Branimir Šimić

University of Zagreb Faculty of Food Technology and Biotechnology, Laboratory for ToxicologyZagreb, Croatia
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Šimić, Branimir
, 
Nina Bilandžić

Nina Bilandžić

Croatian Veterinary Institute, Department of Veterinary Public Health, Laboratory for Residue ControlZagreb, Croatia
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Bilandžić, Nina
, 
Anamaria Brozović

Anamaria Brozović

Ruđer Bošković Institute, Division of Molecular Biology, Laboratory for Cell Biology and SignallingZagreb, Croatia
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Brozović, Anamaria
 et 
Ivana Kmetič

Ivana Kmetič

University of Zagreb Faculty of Food Technology and Biotechnology, Laboratory for ToxicologyZagreb, Croatia
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Kmetič, Ivana
  
30 déc. 2021
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Catégorie d'article: Original article
Publié en ligne: 30 déc. 2021
Pages: 326 - 332
Reçu: 01 sept. 2021
Accepté: 01 nov. 2021
DOI: https://doi.org/10.2478/aiht-2021-72-3588
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© 2021 Heinz-Elmar Tenorth, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

The effects of 10–100 μmol/L PCB 153 on the viability and proliferation of CHO-K1 cells after 6, 24, 48, and 72 h of exposure determined with the Trypan Blue (A), Neutral Red (B), Kenacid Blue (C), and MTT assays (D). Data are presented as mean percentages of control (±SEM). Statistical significance vs control: ap<0.001; bp<0.005; cp<0.01; dp<0.025; ep<0.05
The effects of 10–100 μmol/L PCB 153 on the viability and proliferation of CHO-K1 cells after 6, 24, 48, and 72 h of exposure determined with the Trypan Blue (A), Neutral Red (B), Kenacid Blue (C), and MTT assays (D). Data are presented as mean percentages of control (±SEM). Statistical significance vs control: ap<0.001; bp<0.005; cp<0.01; dp<0.025; ep<0.05

Figure 2

CHO-K1 cell death after 6 h of exposure to 10–100 μmol/L PCB 153. (A) representative dot plot profiles of control and PCB-treated cells; (B) distribution of live, total apoptotic, and dead cells (mean±SEM); (C) distribution of early apoptotic and late apoptotic/dead cells relative to the total number of cells (mean±SEM). Statistical significance vs respective controls: ap<0.001; bp<0.005; cp<0.01; dp<0.025
CHO-K1 cell death after 6 h of exposure to 10–100 μmol/L PCB 153. (A) representative dot plot profiles of control and PCB-treated cells; (B) distribution of live, total apoptotic, and dead cells (mean±SEM); (C) distribution of early apoptotic and late apoptotic/dead cells relative to the total number of cells (mean±SEM). Statistical significance vs respective controls: ap<0.001; bp<0.005; cp<0.01; dp<0.025

Figure 3

CHO-K1 cell death after 48 h of exposure to 10–100 μmol/L PCB 153. (A) representative dot plot profiles of control and PCB-treated cells; (B) distribution of live, total apoptotic, and dead cells (mean±SEM); (C) distribution of early apoptotic and late apoptotic/dead cells relative to the total number of cells (mean±SEM)
CHO-K1 cell death after 48 h of exposure to 10–100 μmol/L PCB 153. (A) representative dot plot profiles of control and PCB-treated cells; (B) distribution of live, total apoptotic, and dead cells (mean±SEM); (C) distribution of early apoptotic and late apoptotic/dead cells relative to the total number of cells (mean±SEM)

Figure 4

CHO-K1 cell cycle analysis after exposure to 50 μmol/L PCB 153. (A) representative flow cytometric histograms of cell cycle progression after 6 and 48-hour exposure (B) Distribution (percentage) of exposed cells across the cell cycle phases over 72 h
CHO-K1 cell cycle analysis after exposure to 50 μmol/L PCB 153. (A) representative flow cytometric histograms of cell cycle progression after 6 and 48-hour exposure (B) Distribution (percentage) of exposed cells across the cell cycle phases over 72 h

Figure 5

Effects of 10–100 μmol/L PCB 153 on ROS formation in CHO-K1 cells. Data are presented as mean percentage of control (±SEM). *p<0.05 vs control
Effects of 10–100 μmol/L PCB 153 on ROS formation in CHO-K1 cells. Data are presented as mean percentage of control (±SEM). *p<0.05 vs control

Inhibition concentrations (μmol/L) determined with four different cytotoxicity assays after 6, 24, 48, and 72 h of CHO-K1 cell exposure to PCB 153

PCB 153 (μmol/L)
6 h 24 h 48 h 72 h
Endpoint IC20 IC50 IC80 IC20 IC50 IC80 IC20 IC50 IC80 IC20 IC50 IC80
TB 32.7 66.3 92.6 23.3 64.5 ND 28.5 66.1 98.3 20.8 55.5 88.6
NR 61.5 ND ND 22.6 65.9 ND 34.0 65.2 89.3 21.7 51.0 78.3
KB 61.0 ND ND 77.4 ND ND 73.0 95.9 ND 85.2 ND ND
MTT 19.7 58.4 98.6 67.8 ND ND 63.4 ND ND 34.8 86.7 ND
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