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Reproductive disorders in male rats induced by high-fructose consumption from juvenile age to puberty

Oleksandr Y. Tkachenko
Oleksandr Y. Tkachenko
, 
Ganna M. Shayakhmetova
Ganna M. Shayakhmetova
, 
Anatoliy V. Matvienko
Anatoliy V. Matvienko
 et 
Valentina M. Kovalenko
Valentina M. Kovalenko
   | 09 avr. 2020
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Article Category: Original article
Publié en ligne: 09 avr. 2020
Pages: 78 - 86
Reçu: 01 juin 2019
Accepté: 01 févr. 2020
DOI: https://doi.org/10.2478/aiht-2020-71-3303
© 2020 Oleksandr Y. Tkachenko, Ganna M. Shayakhmetova, Anatoliy V. Matvienko, Valentina M. Kovalenko, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Photomicrographs of rat testes stained with haematoxylin and eosin; (A) normal structure of seminiferous tubules in control rats (200x magnification); (B) focal dystrophic changes in spermatogonia (spg) and primary spermatocytes (spc) in HF rats (400x magnification); (C) spermatogenic epithelium exfoliation into the lumens of seminiferous tubules in HF rats (200x magnification); (D) decrease in the number of spermatogenic epithelium layers and expansion of the seminiferous tubules in HF rats (400x magnification)
Photomicrographs of rat testes stained with haematoxylin and eosin; (A) normal structure of seminiferous tubules in control rats (200x magnification); (B) focal dystrophic changes in spermatogonia (spg) and primary spermatocytes (spc) in HF rats (400x magnification); (C) spermatogenic epithelium exfoliation into the lumens of seminiferous tubules in HF rats (200x magnification); (D) decrease in the number of spermatogenic epithelium layers and expansion of the seminiferous tubules in HF rats (400x magnification)

Figure 2

Photomicrographs of rats epididymis stained with haematoxylin and eosin; (A and B) normal epididymis tubule structure in control rats (100x and 400x magnification, respectively); (C) focal luminal sperm stasis in HF rats (100x magnification); (D) epithelial clear cell hyperplasia in HF rats (400x magnification)
Photomicrographs of rats epididymis stained with haematoxylin and eosin; (A and B) normal epididymis tubule structure in control rats (100x and 400x magnification, respectively); (C) focal luminal sperm stasis in HF rats (100x magnification); (D) epithelial clear cell hyperplasia in HF rats (400x magnification)

Spermatogenesis epithelium findings

Parameters Groups
 P value
Control High-fructose
Spermatogenesis index (stages of spermatogenesis total / number of examined tubules) 3.44±0.21 3.43 (3.42–3.45) 3.34±0.05 3.34 (3.31–3.38) <0.001*
Number of spermatogonia (per tubular cross section) 71.83±5.43 71.64 (68.38–75.28) 59.80±4.24 59.64 (56.99–62.61) <0.001*
Cells at the 12th stage of spermatogenesis (%) 4.00 (3.0; 4.5) 2.53 2.83(2.03±1.21 –3.64) 0.008*
Exfoliation of epithelium (%) 1.0 (1.0; 2.0) 2.5 (1.5; 3.0) 1.000

Comparison of gonad weight, volume, and sperm counts between the high-fructose-treated and control group

Groups
Parameters Control High-fructose P value
Testes absolute weight (g) 3.48 3.49(3.31±0.24 –3.68) 3.20 3.21(3.06±0.19 –3.36) 0.014*
Testes relative weight (% of body weight) 1.16 1.17(1.10±0.08–1.23) ) 1.27 1.28(1.15±0.17 –1.41) 0.09
Testes volume (cm3) 3.79 3.81(3.53±0.39 –4.10) 3.34 3.37(3.05±0.45 –3.69) 0.029*
Epididymis absolute weight (g) 0.96 (0.95; 0.98) 0.88 0.88(0.81±0.11 –0.95) 0.047*
Epididymis relative weight (% of body weight) 0.32 0.33(0.30±0.04 –0.35) 0.35 0.35(0.32±0.02 –0.38) 0.217
Sperm count (million/mL) 68.43 70.26(53.23±6.96 –87.28) 52.01 52.57(46.31±3.19 –58.82) 0.046*

Comparison of serum sex hormone levels between the high-fructose-treated and control group

Parameters Groups
 P value
Control High-fructose
T (nmol/L) 14.69 14.96(12.09±3.10 –17.82) 9.34 (10.25; 12.25) 0.024*
LH (ng/mL) 0.301±0.231) 0.174 (0.124–0.478) 0.691±0.189 0.622 (0.505–0.797) 0.003*
FSH (ng/mL) 0.077 0.10(0.044±0.06 –0.157) 0.218 (0.171; 0.235) 0.021*

Fertilising capacity of male rats

Groups Number of mated females Number of pregnant females Fertilising capacity
Control 42 38 91 %
High-fructose group 42 41 98 %
P value - - 0.809

Fertility rate of unexposed females mated with HF males

Groups Parameters
Total number of foetuses Number of foetuses per female
Control 319 9.5 (7.5; 10.5)
High-fructose group 300 9 (6; 9)
P value - 0.092

Effects of paternal high-fructose treatment on embryogenesis/foetogenesis in unexposed females on day 20 of gestation

Groups
Parameters Control High-fructose P value
Number of pregnant females (N) 38 41 -
Total number of corpora lutea (N) 362 394 -
Number of corpora lutea per female (N) 10.5 (8.5; 11) 10 (9; 11) 1.000
Preimplantational loss (N /%) 25 / 10 60 / 15 0.012*
Preimplantational loss per female (N) 0 (0; 1) 1 (0; 2) 0.028*
Postimplantational loss (N /%) 6 / 2 19 / 6 0.013*
Postimplantational loss per female (N) 0 (0; 0) 0 (0; 1) 0.041*
Total embryonal/foetal death (%) 11.88 23.86 <0.001*
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