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Figure 1.

Risk factors for T1DM and T2DM
Risk factors for T1DM and T2DM

Sequences of primers, reaction mixture and amplification program used in the study

Bifidobacterium spp. All bacteria

Oligonucleotide sequence (5′−> 3′) F: CTCCTGGAAACGGGTGGR:GGTGTTCTTCCCGATATCTACA F: CCTACGGGNGGCWGCAGR:GACTACHVGGGTATCTAATCC

Reaction mixture Water 2.8 µl
SYBR Green PCR Master Mix 5 µl
Primer F (20mM) 0.1 µl
Primer R (20mM) 0.1 µl
DNA 2 µl

Amplification program 95°C – 5 min 95°C – 5 min
95°C – 30 sec 40 cycles 95°C – 30 sec 40 cycles
53°C – 30 sec 55°C – 30 sec
72°C – 30 sec 72°C – 30 sec

Characteristics of the studied patient groups

T1DMn = 33 T2DMn = 31 CONTROLn = 33 p-value
Sex: male: female (n) 9 : 24 13 : 18 10 : 23 0.424
Age (yrs) 39.36 (± 13.27) 62.68 (± 8.87) 41.21 (± 13.96) <0.001
BMI [kg/m2] 24.27 (± 4.80) 29.28 (± 4.95) 23.62 (± 1.74) <0.001
Glucose [mmol/l] 7.26 (± 1.63) 7.85 (± 2.62) 4.67 (± 0.56) <0.001*
Total cholesterol [mmol/l] 4.66 (± 0.89) 4.71 (± 1.19) 5.17 (± 0.70) 0.025
HDL [mmol/l] 1.68 (± 0.40) 1.12 (± 0.37) 1.65 (± 0.33) <0.001
LDL [mmol/l] 2.60 (± 0.75) 2.70 (± 1.06) 3.09 (± 0.54) 0.009
Triglycerides (TG) [mmol/l] 0.99 (± 0.57) 2.29 (± 1.83) 0.98 (± 0.43) <0.001*
ALT [U/l] 17.73 (± 8.57) 28.58 (± 16.46) 18.92 (± 6.36) <0.001*
HbA1c [%] 8.37 (± 2.72) 8.11 (± 2.72) 5.38 (± 0.24) <0.001
Bifidobacterium spp.[CFU/g][logCFU/g] 7.08 × 106 (± 2.14 × 107)6.13 (± 0.95) 6.38 × 106 (± 9.59 × 106)6.34 (± 0.77) 1.41 × 107 (± 1.76 × 107)6.93 ± (0.43) <0.001*
All bacteria[CFU/g][logCFU/g] 7.27 × 1011 (± 9.04 × 1011)11.32 (± 0.96) 7.29 × 1011 (± 7.57 × 1011)11.49 (± 0.73) 1.48 × 1012 (± 4.09 × 1012)11.58 (± 0.59) 0.397
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