Addition of cells and media to sterile culture dishes under safety cabinet
Fig. 3
Reading the results on the Muse cell analyzer device
Fig. 4
Data graphics obtained from the Muse Cell Analyzer, showing the apoptosis rates of EAT cells according to the groups at the end of 24 hours of incubation. A. Control group, B. 5-FU group, C. DMSO group, D. 50 μg/ml YP group, E. 100 μg/ml YP group, F. 200 μg/ml YP group, G. 400 μg/ml YP group, H. 800 μg/ml YP group
Fig. 5
Percentage values of total apoptosis according to the culture time of the groups
Fig. 6
Data graphics obtained from the Muse Cell Analyzer, showing the apoptosis rates of EAT cells according to the groups at the end of 48 hours incubation. A. Control group, B. 5-FU group, C. DMSO group, D. 50 μg/ml YP group, E. 100 μg/ml YP group, F. 200 μg/ml YP group, G. 400 μg/ml YP group, H. 800 μg/ml YP group
Fig. 7
Data graphics obtained from the Muse Cell Analyzer, showing the apoptosis rates of EAT cells according to the groups at the end of 72 hours of incubation. A. Control group, B. 5-FU group, C. DMSO group, D. 50 μg/ml YP group, E. 100 μg/ml YP group, F. 200 μg/ml YP group, G. 400 μg/ml YP group, H. 800 μg/ml YP group
Fig. 8
Data graphs obtained from Muse Cell Analyzer, showing cell cycle rates of EAT cells according to groups at the end of 48 hours of incubation. A. Control group, B. 5-FU group, C. DMSO group, D. 50 μg/ml YP group, E. 100 μg/ml YP group, F. 200 μg/ml YP group, G. 400 μg/ml YP group, H. 800 μg/ml YP group
Fig. 9
Data graphs obtained from Muse Cell Analyzer, showing cell cycle rates of EAT cells according to groups at the end of 72 hours of incubation. A. Control group, B. 5-FU group, C. DMSO group, D. 50 μg/ml YP group, E. 100 μg/ml YP group, F. 200 μg/ml YP group, G. 400 μg/ml YP group, H. 800 μg/ml YP group
Fig. 10
Percentage values of the G2 / M Stage according to the cultural times of the groups
Fig. 11
Data graphics obtained from the Muse Cell Analyzer, showing the mitotic activity rates of EAT cells according to the groups at the end of 24 hours of incubation. A. Control group, B. 5-FU group, C. DMSO group, D. 50 μg/ml YP group, E. 100 μg/ml YP group, F. 200 μg/ml YP group, G. 400 μg/ml YP group, H. 800 μg/ml YP group
Fig. 12
Data graphics obtained from the Muse Cell Analyzer, showing the mitotic activity rates of EAT cells according to the groups at the end of 48 hours of incubation. A. Control group, B. 5-FU group, C. DMSO group, D. 50 μg/ml YP group, E. 100 μg/ml YP group, F. 200 μg/mlYP group, G. 400 μg/ml YP group, H. 800 μg/ml YP group
Expression of late apoptosis values according to groups and time
Late apoptosis
Incubation times
Groups
24 hours
48 hours
72 hours
Control
32.40±0.44ab
30.46±2.71ab
36.41±6.22a
5 FU
30.46±1.50a
27.5±2.32a
37.13±1.53a
DMSO
33.48±2.17ab
38.61±6.46abc
37.48±1.72a
50 μg/mL
35.76±1.54ab
35.00±2.08ab
37.86±5.73a
100 μg/mL
35.80±0.66ab
35.86±4.75ab
40.95±2.95a
200 μg/mL
44.50±4.18b
42.26±2.78bc
52.66±3.90b
400 μg/mL
42.40±2.06ab
38.91±1.25abc
55.33±3.44b
800 μg/mL
60.76±11.27c
50.20±7.34c
59.88±1.58b
p value between groups
<0.001
<0.001
<0.001
Expressing the proportional values of total depolarized cells by groups and time
Total Amount of Depolarized
Incubation times
Groups
24 hour
48 hour
72 hour
Control
0.11±0.10a
0.05±0.05ab
0.25±0.10a
5 FU
0.10±0.00a
0.00±0.00a
0.36±0.10a
DMSO
0.28±0.12a
0.03±0.05a
0.25±0.31a
50 μg/mL Y
0.46±0.33ab
0.06±0.02ab
0.28±0.40a
100 μg/mL Y
0.61±0.05ab
0.13±0.02ab
0.40±0.22a
200 μg/mL Y
0.91±0.05ab
0.08±0.02ab
0.33±0.25a
400 μg/mL Y
1.78±0.96bc
0.41±0.23b
0.50±0.44a
800 μg/mL Y
2.96±0.83c
0.86±0.27c
4.80±4.56a
p value between groups
<0.001
<0.001
<0.042
Expression of G2/M phase proportional values according to groups and time
G2/M
Incubation times
Groups
24 hours
48 hours
72 hours
Control
8.20±1.77a
10.96±1.41ab
17.26±3.90a
5 FU
7.06±1.02a
9.50±2.27b
9.75±2.89c
DMSO
6.20±0.72a
15.13±2.15ac
14.73±1.62ac
50 μg/mL
7.13±0.58a
18.53±1.92c
16.23±2.35ac
100 μg/mL
8.06±1.19a
12.03±1.26ab
11.90±0.91ac
200 μg/mL
8.03±0.11a
13.03±1.28ab
13.96±0.70ac
400 μg/mL
7.63±0.45a
11.93±2.04ab
11.06±0.35bc
800 μg/mL
8.50±1.01a
10.60±2.30ab
11.20±1.40bc
p value between groups
0.158
<0.001
<0.005
Expression of early apoptosis values by groups and time
Early apoptosis
Incubation times
Groups
24 hours
48 hours
72 hours
Control
65.40±0.20a
45.83±0.42ab
32.75±33.80a
5 FU
67.50±1.37a
53.50±4.47a
27.78±1.02ab
DMSO
63.35±2.55a
41.31±3.54ab
22.83±6.29abc
50 μg/mL Y
61.01±2.10ab
49.15±2.05ad
29.63±5.17a
100 μg/mL Y
60.43±1.03ab
44.13±5.41ae
28.40±1.40ab
200 μg/mL Y
49.05±5.36b
35.41±3.67bce
17.55±3.74bc
400 μg/mL Y
49.13±2.22b
40.76±1.71bde
17.60±3.03bc
800 μg/mL Y
21.80±11.87c
27.55±8.80c
14,60±4,32c
p value between groups
<0.001
<0.001
<0,001
Expression of total apoptosis values according to groups and time