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Assessment of a multiplex RT-PCR for Simultaneous, Rapid Screening of Common Viral Infections of Central Nervous System: A Prospective Study for Enteroviruses and Herpesviruses

Maryam Khalili

Maryam Khalili

Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical ScienceIsfahan, Iran
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Khalili, Maryam
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Hamid Rahimi Hajiabadi

Hamid Rahimi Hajiabadi

Pediatric Department, School of Medicine, Isfahan University of Medical SciencesIsfahan, Iran
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Hajiabadi, Hamid Rahimi
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Mojtaba Akbari

Mojtaba Akbari

Isfahan Endocrine and Metabolism Research center, Isfahan University of Medical ScienceIsfahan, Iran
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Akbari, Mojtaba
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Rana Saleh

Rana Saleh

Pediatric Department, School of Medicine, Isfahan University of Medical SciencesIsfahan, Iran
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Saleh, Rana
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Bahram Nasr Esfahani

Bahram Nasr Esfahani

Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical ScienceIsfahan, Iran
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Esfahani, Bahram Nasr
 et 
Sharareh Moghim

Sharareh Moghim

Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical ScienceIsfahan, Iran
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Moghim, Sharareh
  
02 avr. 2022
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Catégorie d'article: Original Study
Publié en ligne: 02 avr. 2022
Pages: 91 - 96
Reçu: 25 mai 2021
Accepté: 29 oct. 2021
DOI: https://doi.org/10.2478/ahem-2022-0011
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© 2022 Maryam Khalili et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

The multiplex PCR assay’s specificity was examined using PCR mixes containing all two-primer pairs and a single template (cDNA/DNA) from the positive controls. Lane 1: standard 100-bp DNA ladder marker. Lane 2: the mixture of enterovirus (440 bp) and HSV (292 bp) positive controls. Lane 3: enterovirus (440 bp), Lane 4: HSV (292 bp), Lane 5: negative control (E. coli)
The multiplex PCR assay’s specificity was examined using PCR mixes containing all two-primer pairs and a single template (cDNA/DNA) from the positive controls. Lane 1: standard 100-bp DNA ladder marker. Lane 2: the mixture of enterovirus (440 bp) and HSV (292 bp) positive controls. Lane 3: enterovirus (440 bp), Lane 4: HSV (292 bp), Lane 5: negative control (E. coli)

Fig. 2

Comparison of the sensitivity between multiplex PCR and monoplex PCR for the 10-fold serial dilutions of positive controls. (A): HSV (292 bp) (B): Enteroviruses (440 bp)
Comparison of the sensitivity between multiplex PCR and monoplex PCR for the 10-fold serial dilutions of positive controls. (A): HSV (292 bp) (B): Enteroviruses (440 bp)
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Sujets de la revue:
Sciences de la vie, Biologie moléculaire, Microbiologie et virologie, Médecine, Sciences médicales de base, Immunologie
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