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Expression and biochemical significance of Piwil2 in stem cell lines

Dilek Kaan

Dilek Kaan

Genome and Stem Cell Center, University of Erciyes KayseriTurkey
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Kaan, Dilek
  
02 avr. 2022
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Catégorie d'article: Original Study
Publié en ligne: 02 avr. 2022
Pages: 97 - 103
Reçu: 20 avr. 2021
Accepté: 23 sept. 2021
DOI: https://doi.org/10.2478/ahem-2022-0009
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© 2022 Dilek Kaan, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

A. Morphologies of MCF10A cells with inverted microscope. B. MCF-7cells (x200 magnification)
A. Morphologies of MCF10A cells with inverted microscope. B. MCF-7cells (x200 magnification)

Fig. 2

A. Comparison of GST A1 activities of non-transfected MCF10A cells and MCF10A/Piwil2 cells by using CDNB as substrate. B. Comparison of GST A1 activities of non-transfected MCF-7 cells and MCF-7/Piwil2 by using CDNB as substrate.
A. Comparison of GST A1 activities of non-transfected MCF10A cells and MCF10A/Piwil2 cells by using CDNB as substrate. B. Comparison of GST A1 activities of non-transfected MCF-7 cells and MCF-7/Piwil2 by using CDNB as substrate.

Fig. 3

A. Comparison of GST P1 activities of non-transfected MCF10A cells and MCF10A/Piwil2, B. Comparison of GST P1 activities of non-transfected MCF-7 cells and MCF-7/Piwil2. Error bars represent standard deviation from the mean (┬)
A. Comparison of GST P1 activities of non-transfected MCF10A cells and MCF10A/Piwil2, B. Comparison of GST P1 activities of non-transfected MCF-7 cells and MCF-7/Piwil2. Error bars represent standard deviation from the mean (┬)

Fig. 4

Molecular characterization with fluorescence microscopy of cell lines. A. Non-transfected MCF10A. B. Non-transfected MCF-7. (Cells expressing Piwil2 are indicated by arrows) (FITC, green signal) (x200 magnification).
Molecular characterization with fluorescence microscopy of cell lines. A. Non-transfected MCF10A. B. Non-transfected MCF-7. (Cells expressing Piwil2 are indicated by arrows) (FITC, green signal) (x200 magnification).

Fig. 5

A. Immunocytochemistry of Piwil2 transfected cell line MCF10A with an antibody against Piwil2 protein. B. Immunocytochemistry of Piwil2 transfected cell line MCF-7 with an antibody against Piwil2 protein (Cells expressing Piwil2 are indicated by arrows) (FITC, green signal) (x200 magnification).
A. Immunocytochemistry of Piwil2 transfected cell line MCF10A with an antibody against Piwil2 protein. B. Immunocytochemistry of Piwil2 transfected cell line MCF-7 with an antibody against Piwil2 protein (Cells expressing Piwil2 are indicated by arrows) (FITC, green signal) (x200 magnification).
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Anglais
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Sujets de la revue:
Sciences de la vie, Biologie moléculaire, Microbiologie et virologie, Médecine, Sciences médicales de base, Immunologie
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