<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The inoculation of plant species with mycorrhiza fungus <italic>Piriformospora indica</italic> results in enhancement of growth, increase in yield, and induction of resistance to biotic and abiotic diseases through improvement of the root system. The aim of the present study was to optimize <italic>in vitro</italic> propagation protocol for three indigenous apples (<italic>Malus × domestica</italic>) cultivars (ꞌGolbaharꞌ, ꞌSharbatiꞌ, ꞌSoltani Shabestariꞌ) and one commercial cultivar (ꞌGolden Deliciousꞌ). Furthermore, the efficiency of <italic>P. indica</italic> at rooting stage was investigated on three cultivars (ꞌSharbatiꞌ, ꞌSoltani Shabestariꞌ, ꞌGolden Deliciousꞌ). Establishment and proliferation stages were optimized by collecting explants at different seasons and comparing different culture media respectively. Rooting optimization included six treatments containing different concentrations of auxins in the presence or absence of <italic>P. indica</italic>. Results showed that at the establishment stage, a maximum percent of survival was observed in explants collected in spring. At the proliferation stage, different media had a divergent effects on distinct cultivars. Although all cultivars reacted favourable to micropropagation in MS (Murashige &amp; Skoog 1962) basal medium, the presence or absence of cytokinin 2ip (N<sub>6</sub>-(2-Isopentenyl) adenine) in the culture media showed significant and incremental improvements in growth indices. In all cultivars highest rooting percent, root length, root thickness, and the number of roots/explant was observed in MS media containing auxins for three weeks followed by a treatment of MS medium containing <italic>P. indica</italic> for another three weeks. Plantlets treated with <italic>P. indica</italic>, grow stronger and healthier at the acclimation stage compared to the ones that excluded <italic>P. indica</italic>.</p></abstract>

The inoculation of plant species with mycorrhiza fungus Piriformospora indica results in enhancement of growth, increase in yield, and induction of resistance to biotic and abiotic diseases through improvement of the root system. The aim of the present study was to optimize in vitro propagation protocol for three indigenous apples (Malus × domestica) cultivars (ꞌGolbaharꞌ, ꞌSharbatiꞌ, ꞌSoltani Shabestariꞌ) and one commercial cultivar (ꞌGolden Deliciousꞌ). Furthermore, the efficiency of P. indica at rooting stage was investigated on three cultivars (ꞌSharbatiꞌ, ꞌSoltani Shabestariꞌ, ꞌGolden Deliciousꞌ). Establishment and proliferation stages were optimized by collecting explants at different seasons and comparing different culture media respectively. Rooting optimization included six treatments containing different concentrations of auxins in the presence or absence of P. indica. Results showed that at the establishment stage, a maximum percent of survival was observed in explants collected in spring. At the proliferation stage, different media had a divergent effects on distinct cultivars. Although all cultivars reacted favourable to micropropagation in MS (Murashige & Skoog 1962) basal medium, the presence or absence of cytokinin 2ip (N6-(2-Isopentenyl) adenine) in the culture media showed significant and incremental improvements in growth indices. In all cultivars highest rooting percent, root length, root thickness, and the number of roots/explant was observed in MS media containing auxins for three weeks followed by a treatment of MS medium containing P. indica for another three weeks. Plantlets treated with P. indica, grow stronger and healthier at the acclimation stage compared to the ones that excluded P. indica.

Optimizing Micropropagation of Apple (<italic>Malus</italic> × <italic>Domestica</italic> Borkh) and <italic>in Vitro</italic> Root Induction By <italic>Piriformospora indica</italic>

Agriculture (Pol'nohospodárstvo)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Optimizing Micropropagation of Apple ( ×  Borkh) and  Root Induction By 

The inoculation of plant species with mycorrhiza fungus Piriformospora indica results in enhancement of growth, increase in yield, and induction of resistance...

Optimizing Micropropagation of Apple (Malus × Domestica Borkh) and in Vitro Root Induction By Piriformospora indica

Publié en ligne: 29 janv. 2021

Pages: 137 - 147

Reçu: 28 juin 2020

Accepté: 14 déc. 2020

DOI: https://doi.org/10.2478/agri-2020-0013

Mots clés
apple cultivars, culture media, establishment, proliferation

© 2020 Samira Masoudi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Optimizing Micropropagation of Apple (Malus × Domestica Borkh) and in Vitro Root Induction By Piriformospora indica

Publié en ligne: 29 janv. 2021

Pages: 137 - 147

Reçu: 28 juin 2020

Accepté: 14 déc. 2020

DOI: https://doi.org/10.2478/agri-2020-0013

Mots clésapple cultivars, culture media, establishment, proliferation

© 2020 Samira Masoudi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
apple cultivars, culture media, establishment, proliferation