<abstract xmlns="http://www.w3.org/1999/xhtml">

It was known that hawthorn - <italic>Crataegus</italic> L. is a polymorphic genus. Two hawthorn species and their hybrids are included in the European Pharmacopoeia, twelve – in Ukrainian pharmacopoeia. Determination of chromatographic profiles of hawthorn fruits species native to Ukraine and other countries that are non-pharmacopoeial, but have sufficient plant raw material base, is essential for quality control of drugs.
<bold>Aim.</bold> To analyze and compare the chromatographic profiles of fruits of 23 <italic>Crataegus</italic> L. species on phenolic compounds, evaluated by means of high-performance thin-layer chromatography procedure (HPTLC), and determine the specific features of chromatographic fingerprints.
<bold>Materials and Methods.</bold> A total of 39 samples of fruits of 23 hawthorn species that are native to Europe, Asia and North America, such as <italic>Crataegus monogyna</italic>, <italic>C. laevigata/C. oxyacantha, C. leiomonogyna</italic>, <italic>C. curvisepala</italic>, <italic>C. pseudokyrtostyla</italic>, <italic>C. fallacina</italic>, <italic>C. subrotunda</italic>, <italic>C. ambigua</italic>, <italic>C. pentagyna</italic>, <italic>C. sanguinea</italic>, <italic>C. chlorosarca</italic>, <italic>C. almaatensis</italic>, <italic>C.pseudoheterophylla</italic> subsp. <italic>turkestanica</italic>, <italic>C. pinnatifida</italic>, <italic>C. pentagyna</italic> subsp. <italic>pseudomelanocarpa</italic>, <italic>C. punctata, C. pringlei, C. festiva</italic>, <italic>C. douglasii, C. holmesiana</italic>, <italic>C. submollis</italic>, <italic>C. flabellata</italic>, <italic>C. canadensis</italic> were investigated. The analysis has been done following the TLC method from European Pharmacopeia modified into HPTLC, using automated HPTLC herbal system (CAMAG, Switzerland).
<bold>The results</bold> have shown that chromatographic profiles of phenolic constituents of nine <italic>Crataegus</italic> L. species of Europe, both pharmacopoeial and non-pharmacopoeial, were quite similar, despite the significant morphological distinctions. The chromatographical profiles of three species of Asia were similar to the pharmacopoeial species; three other species looked different and had specific marker zones. In addition, eight <italic>Crataegus</italic> L. species of North America had specific markers helping for discriminative analysis from pharmacopoeial species.
<bold>Conclusion.</bold> The findings could help to identify the possible adulterations and prevent the falsification of finished products. The results will be taken into consideration during revision of the Ukrainian national pharmacopoeial monograph for hawthorn fruits.
</abstract>

It was known that hawthorn - Crataegus L. is a polymorphic genus. Two hawthorn species and their hybrids are included in the European Pharmacopoeia, twelve – in Ukrainian pharmacopoeia. Determination of chromatographic profiles of hawthorn fruits species native to Ukraine and other countries that are non-pharmacopoeial, but have sufficient plant raw material base, is essential for quality control of drugs.
Aim. To analyze and compare the chromatographic profiles of fruits of 23 Crataegus L. species on phenolic compounds, evaluated by means of high-performance thin-layer chromatography procedure (HPTLC), and determine the specific features of chromatographic fingerprints.
Materials and Methods. A total of 39 samples of fruits of 23 hawthorn species that are native to Europe, Asia and North America, such as Crataegus monogyna, C. laevigata/C. oxyacantha, C. leiomonogyna, C. curvisepala, C. pseudokyrtostyla, C. fallacina, C. subrotunda, C. ambigua, C. pentagyna, C. sanguinea, C. chlorosarca, C. almaatensis, C.pseudoheterophylla subsp. turkestanica, C. pinnatifida, C. pentagyna subsp. pseudomelanocarpa, C. punctata, C. pringlei, C. festiva, C. douglasii, C. holmesiana, C. submollis, C. flabellata, C. canadensis were investigated. The analysis has been done following the TLC method from European Pharmacopeia modified into HPTLC, using automated HPTLC herbal system (CAMAG, Switzerland).
The results have shown that chromatographic profiles of phenolic constituents of nine Crataegus L. species of Europe, both pharmacopoeial and non-pharmacopoeial, were quite similar, despite the significant morphological distinctions. The chromatographical profiles of three species of Asia were similar to the pharmacopoeial species; three other species looked different and had specific marker zones. In addition, eight Crataegus L. species of North America had specific markers helping for discriminative analysis from pharmacopoeial species.
Conclusion. The findings could help to identify the possible adulterations and prevent the falsification of finished products. The results will be taken into consideration during revision of the Ukrainian national pharmacopoeial monograph for hawthorn fruits.

Chromatographic Profiles Analysis of Fruits of <italic>Crataegus</italic> L. Genus by High-Performance Thin-Layer Chromatography

Botanical Garden of V.N. Karazin Kharkiv National University

European Pharmaceutical Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Chromatographic Profiles Analysis of Fruits of  L. Genus by High-Performance Thin-Layer Chromatography

It was known that hawthorn - Crataegus L. is a polymorphic genus. Two hawthorn species and their hybrids are included in the European Pharmacopoeia, twelve –...

Chromatographic Profiles Analysis of Fruits of Crataegus L. Genus by High-Performance Thin-Layer Chromatography

Publié en ligne: 28 janv. 2020

Pages: 45 - 51

Reçu: 23 avr. 2018

Accepté: 01 oct. 2018

DOI: https://doi.org/10.2478/afpuc-2019-0020

Mots clés
Hawthorn, identification, phenolic compounds, high-performance thin-layer chromatography

© 2019 K.O. Khokhlova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Chromatographic Profiles Analysis of Fruits of Crataegus L. Genus by High-Performance Thin-Layer Chromatography

Publié en ligne: 28 janv. 2020

Pages: 45 - 51

Reçu: 23 avr. 2018

Accepté: 01 oct. 2018

DOI: https://doi.org/10.2478/afpuc-2019-0020

Mots clésHawthorn, identification, phenolic compounds, high-performance thin-layer chromatography

© 2019 K.O. Khokhlova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
Hawthorn, identification, phenolic compounds, high-performance thin-layer chromatography