<abstract xmlns="http://www.w3.org/1999/xhtml"> A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %.</abstract>

A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %.

HPTLC determination of diosgenin in fenugreek seeds

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"HPTLC determination of diosgenin in fenugreek seeds"}

A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin...

HPTLC determination of diosgenin in fenugreek seeds

Publié en ligne: 16 févr. 2018

Pages: 97 - 107

Accepté: 15 oct. 2017

DOI: https://doi.org/10.2478/acph-2018-0002

Mots clés
fenugreek (Trigonella foenum-graecum), diosgenin, HPTLC, quantitative analysis

© by Mirosława Krauze-Baranowska

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

HPTLC determination of diosgenin in fenugreek seeds

Publié en ligne: 16 févr. 2018

Pages: 97 - 107

Accepté: 15 oct. 2017

DOI: https://doi.org/10.2478/acph-2018-0002

Mots clésfenugreek (Trigonella foenum-graecum), diosgenin, HPTLC, quantitative analysis

© by Mirosława Krauze-Baranowska

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
fenugreek (Trigonella foenum-graecum), diosgenin, HPTLC, quantitative analysis