Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification

1. K. M. Nadkarni, Indian Materia Medica, Popular Prakashan Pvt. Ltd., Vol. 1, Bombay 1976, pp. 946-948.Search in Google Scholar

2. J. B. Calixto, A. R. S. Santos, V. C. Filho and R. A. Yunes, A review of the plants of genus Phyllanthus: Their chemistry, pharmacology and therapeutic potential, Med. Res. Rev. 18 (1998) 225-258; DOI: 10.1002/(SICI)1098-1128(199807)18:4<225::AID-MED2>3.0.CO;2-X.10.1002/(SICI)1098-1128(199807)18:4<225::AID-MED2>3.0.CO;2-XSearch in Google Scholar

3. S. Khatoon, V. Rai, A. K. S. Rawat and S. Mehrotra, Comparative pharmacognostic studies of three Phyllanthus species, J. Ethnopharmacol. 104 (2006) 79-86; DOI: 10.1016/j.jep.2005.08.048.10.1016/j.jep.2005.08.048Search in Google Scholar

4. P. Padma and O. H. Setty, Protective effect of Phyllanthus fraternus against carbon tetrachloride- -induced mitochondrial dysfunction, Life Sci. 64 (1999) 2411-2417; DOI: 10.1016/S0024-3205(99) 00195-2.Search in Google Scholar

5. H. Chirdchupunseree and P. Pramyothin, Protective activity of phyllanthin in ethanol-treated primary culture of rat hepatocytes, J. Ethnopharmacol. 128 (2010) 172-176; DOI: 10.1016/j.jep. 2010.01.003.Search in Google Scholar

6. R. Krithika, R. Mohankumar, R. J. Verma, P. S. Shrivastav, I. L. Mohamad, P. Gunasekaran and S. Narasimhan, Isolation, characterization and antioxidative effect of phyllanthin against CCl4-induced toxicity in HepG2 cell line, Chem. Biol. Inter. 181 (2009) 351-358; DOI: 10.1016/j.cbi 2009.06.014.Search in Google Scholar

7. V. Murugaiyah and K. L. Chan, Antihyperuricemic lignans from the leaves of Phyllanthus niruri, Planta Med. 72 (2006) 1262-1267; DOI: 10.1055/s-2006-947224.10.1055/s-2006-947224Search in Google Scholar

8. A. Mazumder, A. Mahato and R. Mazumder, Antimicrobial potentiality of Phyllanthus amarus against drug resistant pathogens, Nat. Prod. Res. 20 (2006) 323-326; DOI: 10.1080/14786410600650404.10.1080/14786410600650404Search in Google Scholar

9. B. Gowrishanker and O. S. Vivekanandan, In-vivo studies of a crude extract of Phyllanthus amarus L. in modifying the genotoxicity induced in Vicia faba L. by tannery effluents, Mutat. Res--Genet. Tox. 322 (1994) 185-192.10.1016/0165-1218(94)90005-1Search in Google Scholar

10. C. A. L. Kassuya, D. F. P. Leite, L. V. De Melo, V. L. G. Rehder and J. B. Calixto, Anti-inflammatory properties of extracts, fractions and lignans isolated from Phyllanthus amarus, Planta Med. 71 (2005) 721-726; DOI: 10.1055/s-2005-871258.10.1055/s-2005-871258Search in Google Scholar

11. M. Inchoo, H. Chirdchupunseree, P. Pramyothin and S. Jianmongkol, Endothelium-independent effects of phyllanthin and hypophyllanthin on vascular tension, Fitoterapia 82 (2011) 1231-1236; DOI: 10.1016/j.fitote.2011.08.013.10.1016/j.fitote.2011.08.013Search in Google Scholar

12. A. K. Tripathi, R. K. Verma, A. K. Gupta, M. M. Gupta and S. P. S. Khanuja, Quantitative determination of phyllanthin and hypophyllanthin in Phyllanthus species by high-performance thin layer chromatography, Phytochem. Anal. 17 (2006) 394-397; DOI: 10.1002/pca.936.10.1002/pca.936Search in Google Scholar

13. A. Annamalai and P. T. V. Lakshmi, HPTLC and HPLC analysis of bioactive phyllanthin from different organs of Phyllanthus amarus, Asian J. Biotechnol. 1 (2009) 154-162; DOI: 10.3923/ajbkr. 2009.154.162.Search in Google Scholar

14. J. Kasthuri, K. Kathiravan and N. Rajendiran, Phyllanthin-assisted biosynthesis of silver and gold nanoparticles: a novel biological approach, J. Nanopart. Res. 11 (2009) 1075-1085; DOI: 10.1007/ s11051-008-9494-9.10.1007/s11051-008-9494-9Search in Google Scholar

15. V. Murugaiyah and K. L. Chan, Determination of four lignans in Phyllanthus niruri L. by a simple high-performance liquid chromatography method with fluorescence detection, J. Chrom. A 1154 (2007) 198-204; DOI: 10.1016/j.chroma.2007.03.079.10.1016/j.chroma.2007.03.079Search in Google Scholar

16. A. Alvari, M. S. Ohadi Rafsanjani, F. J. Ahmed, Mohd S. Hejazi and M. Z. Abdin, Rapid RP-HPLC technique for the determination of phyllanthin as bulk and its quantification in Phyllanthus amarus extract, Int. J. Pytomed. 3 (2011) 115-119.Search in Google Scholar

17. K. Shanker, M. Singh, V. Srivastava, R. K. Verma, A. K. Gupta and M. M. Gupta, Simultaneous analysis of six bioactive lignans in Phyllanthus species by reversed phase hyphenated high performance liquid chromatographic technique, Acta Chromatogr. 23 (2011) 321-337; DOI: 10.1556/ AChrom.23.2011.2.10.10.1556/AChrom.23.2011.2.10Search in Google Scholar

18. C. W. Huie, A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants, Anal. Bioanal. Chem. 373 (2002) 23-30; DOI: 10.1007/s00216-002-1265-3.10.1007/s00216-002-1265-3Search in Google Scholar

19. D. P. Fulzele and R. K. Satdive, Comparison of techniques for the extraction of the anti-cancer drug camptothecin from Nothapodytes foetida, J. Chrom. A 1063 (2005) 9-13; DOI: 10.1016/j.chroma. 2004.11.020.Search in Google Scholar

20. E. Reverchon and I. De Marco, Supercritical fluid extraction and fractionation of natural matter, J. Supercrit. Fluids 38 (2006) 146-166; DOI: 10.1016/j.supflu.2006.03.020.10.1016/j.supflu.2006.03.020Search in Google Scholar

21. P. Hamrapurkar, S. Pawar and M. Phale, Quantitative HPTLC analysis of phyllanthin in Phyllanthusamarus, J. Planar. Chromatogr. 23 (2010) 112-115; DOI: 10.1556/JPC.23.2010.2.4.10.1556/JPC.23.2010.2.4Search in Google Scholar

22. S. Khan, F. Al-Qurainy, M. Ram, S. Ahmad and M. Z. Abdin, Phyllanthin biosynthesis in Phyllanthusamarus: Schum and Thonn growing at different altitudes, J. Med. Plants Res. 4 (2010) 41-48.Search in Google Scholar

23. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Textand Methodology Q2(R1), Current Step 4 version, Geneva, November 2005; http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf; last access date February 1, 2012Search in Google Scholar

24. D. C. Montgomery, Design and Analysis of Experiments, 4th ed., Wiley, New York 1997. Search in Google Scholar

25. R. E. Santelli, M. A. Bezerra, O. D. Sant Ana, R. J. Cassella and S. L. C. Ferreira, Multivariate technique for optimization of digestion procedure by focused microwave system for determination of Mn, Zn and Fe in food samples using FAAS, Talanta 68 (2006) 1083-1088; DOI: 10.1016/ j.talanta.2005.07.010.10.1016/j.talanta.2005.07.010Search in Google Scholar

26. S. Buco, M. Morgagues, M. Sergent, P. Doumenq and G. Mille, An experimental design approach for optimizing polycyclic aromatic hydrocarbon analysis in contaminated soil by pyrolyser-gas chromatography-mass spectrometry, Environ. Res. 104 (2007) 209-215; DOI: 10.1016/j.envres. 2004.06.008.Search in Google Scholar

27. A. A. Patil, B. S. Sachin, D. B. Shinde and P. S. Wakte, Supercritical CO2 assisted extraction and LC-MS identification of picroside I and picroside II from Picrorhiza kurroa, Phytochem. Anal. 24 (2013) 97-104; DOI: 10.1002/pca.2383.10.1002/pca.2383Search in Google Scholar

28. A. Erkucuk, I. H. Akgun and O. Yesil-Celiktas, Supercritical CO2 extraction of glycosides from Stevia rebaudiana leaves: Identification and optimization, J. Supercrit. Fluids 51 (2009) 29-35; DOI: 10.1016/j.supflu.2009.07.002.10.1016/j.supflu.2009.07.002Search in Google Scholar

29. National Institute of Standards and Technology, Thermophysical properties of fluid systems, NIST, 2011; http://webbook.nist.gov/chemistry/fluid/; last access date May 29, 2012Search in Google Scholar

30. M. L. Jeong and D. J. Chesney, Investigation of modifier effects in supercritical CO2 extraction from various solid matrices, J. Supercrit. Fluids 16 (1999) 33-42; DOI: 10.1016/S0896-8446(99)00015-7. 10.1016/S0896-8446(99)00015-7Search in Google Scholar