<abstract xmlns="http://www.w3.org/1999/xhtml">Cell encapsulation utilizing biodegradable material has promising outcomes for tissue engineering. From a long time ago, alginate has been generally utilized for drug delivery, cell transplantation and as a scaffold in biomedical applications. The aim of this study was the comparison of cell viability in the presence of two polymerizing ions: Ba2+ and Ca2+ to improvement the quality of alginate scaffold. For this purpose, WJMSCs after three passage were encapsulated in alginate scaffold in the presence of Ba2+ and ca2+. Cell viability was evaluated by WST-8 assay kit after 24, 48 and 72 hours. The results showed that encapsulated cells in the presence of Ca2+ had more viability than Ba2+. It was also found that using the WST-8 assay kit is a convenient and fast method for evaluation the viability of cells. It can be claimed that Calcl2 polymerizing solution provides more favorable conditions for cell viability compared to Bacl2 solution.<bold>Running title:</bold> Assessing the viability of stem cells by WST-8 assay kit</abstract>

Cell encapsulation utilizing biodegradable material has promising outcomes for tissue engineering. From a long time ago, alginate has been generally utilized for drug delivery, cell transplantation and as a scaffold in biomedical applications. The aim of this study was the comparison of cell viability in the presence of two polymerizing ions: Ba2+ and Ca2+ to improvement the quality of alginate scaffold. For this purpose, WJMSCs after three passage were encapsulated in alginate scaffold in the presence of Ba2+ and ca2+. Cell viability was evaluated by WST-8 assay kit after 24, 48 and 72 hours. The results showed that encapsulated cells in the presence of Ca2+ had more viability than Ba2+. It was also found that using the WST-8 assay kit is a convenient and fast method for evaluation the viability of cells. It can be claimed that Calcl2 polymerizing solution provides more favorable conditions for cell viability compared to Bacl2 solution.Running title: Assessing the viability of stem cells by WST-8 assay kit

Assessment of viability of wharton's jelly mesenchymal stem cells encapsulated in alginate scaffold by WST-8 assay kit

Department of Anatomical Sciences, Faculty of Medicine

Ahvaz Jundishapur University of Medical Sciences

Department of Medical Genetics, Faculty of Medicine

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Cell encapsulation utilizing biodegradable material has promising outcomes for tissue engineering. From a long time ago, alginate has been generally utilized...

Assessment of viability of wharton's jelly mesenchymal stem cells encapsulated in alginate scaffold by WST-8 assay kit

Publié en ligne: 30 mars 2021

Pages: 42 - 47

Reçu: 22 févr. 2021

Accepté: 13 mars 2021

DOI: https://doi.org/10.2478/acb-2021-0007

Mots clés
Wharton's jelly, mesenchymal stem cells, alginate, viability

© 2021 Zeinab Shafiei Seifabadi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Assessment of viability of wharton's jelly mesenchymal stem cells encapsulated in alginate scaffold by WST-8 assay kit

Publié en ligne: 30 mars 2021

Pages: 42 - 47

Reçu: 22 févr. 2021

Accepté: 13 mars 2021

DOI: https://doi.org/10.2478/acb-2021-0007

Mots clésWharton's jelly, mesenchymal stem cells, alginate, viability

© 2021 Zeinab Shafiei Seifabadi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
Wharton's jelly, mesenchymal stem cells, alginate, viability