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Genes encoding proteins regulating fatty acid metabolism and cellular response to lipids are differentially expressed in porcine luminal epithelium during long-term culture

Magdalena Kulus
Magdalena Kulus
, 
Blanka Borowiec
Blanka Borowiec
, 
Małgorzata Popis
Małgorzata Popis
, 
Piotr Celichowski
Piotr Celichowski
, 
Michal Jeseta
Michal Jeseta
, 
Dorota Bukowska
Dorota Bukowska
, 
Hanna Piotrowska-Kempisty
Hanna Piotrowska-Kempisty
, 
Małgorzata Bruska
Małgorzata Bruska
, 
Maciej Zabel
Maciej Zabel
, 
Michał Nowicki
Michał Nowicki
, 
Bartosz Kempisty
Bartosz Kempisty
 et 
Paweł Antosik
Paweł Antosik
   | 12 oct. 2019
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Publié en ligne: 12 oct. 2019
Pages: 58 - 65
Reçu: 11 août 2019
Accepté: 28 août 2019
DOI: https://doi.org/10.2478/acb-2019-0008
Mots clés
© 2019 Magdalena Kulus, Blanka Borowiec, Małgorzata Popis, Piotr Celichowski, Michal Jeseta, Dorota Bukowska, Hanna Piotrowska-Kempisty, Małgorzata Bruska, Maciej Zabel, Michał Nowicki, Bartosz Kempisty, Paweł Antosik, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Heat map representation of differentially expressed genes belonging to the to “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. Arbitrary signal intensity acquired from microarray analysis is represented by colours (green, higher; red, lower expression). Log2 signal intensity values for any single gene were resized to Row Z-Score scale (from -2, the lowest expression to +2, the highest expression for single gene)
Heat map representation of differentially expressed genes belonging to the to “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. Arbitrary signal intensity acquired from microarray analysis is represented by colours (green, higher; red, lower expression). Log2 signal intensity values for any single gene were resized to Row Z-Score scale (from -2, the lowest expression to +2, the highest expression for single gene)

Figure 2

The circle plot showing the differently expressed genes and z-score of the “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. The outer circle shows a scatter plot for each term of the fold change of the assigned genes. Green circles display up- regulation and red ones down- regulation. The inner circle shows the z-score of each GO BP term. The width of the each bar corresponds to the number of genes within GO BP term and the color corresponds to the z-score
The circle plot showing the differently expressed genes and z-score of the “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. The outer circle shows a scatter plot for each term of the fold change of the assigned genes. Green circles display up- regulation and red ones down- regulation. The inner circle shows the z-score of each GO BP term. The width of the each bar corresponds to the number of genes within GO BP term and the color corresponds to the z-score

Figure 3

The representation of the mutual relationship of differently expressed genes that belongs to the “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. The ribbons indicate which gene belongs to which categories. The middle circle represents logarithm from fold change (LogFC) between D7/D1, D15/D1 and D30/D1 respectively. The color of each block corresponds to the LogFC of each gene (green – up-regulated, red – down-regulated). The genes were sorted by logFC from most to least changed gene
The representation of the mutual relationship of differently expressed genes that belongs to the “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. The ribbons indicate which gene belongs to which categories. The middle circle represents logarithm from fold change (LogFC) between D7/D1, D15/D1 and D30/D1 respectively. The color of each block corresponds to the LogFC of each gene (green – up-regulated, red – down-regulated). The genes were sorted by logFC from most to least changed gene

Figure 4

Heatmap showing the gene occurrence between differently expressed genes that belongs to the “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. The yellow color is associated with gene occurrence in the GO Term. The intensity of the color is corresponding to amount of GO BP terms that each gene belongs to
Heatmap showing the gene occurrence between differently expressed genes that belongs to the “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. The yellow color is associated with gene occurrence in the GO Term. The intensity of the color is corresponding to amount of GO BP terms that each gene belongs to

Figure 5

STRING-generated interaction occurrence between differently expressed genes that belongs to the “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. The intensity of the edges reflects the strength of interaction score
STRING-generated interaction occurrence between differently expressed genes that belongs to the “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. The intensity of the edges reflects the strength of interaction score

Figure 6

Functional interaction (FI) between differently expressed genes that belongs to the “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. In following figure “->” stands for activating/catalyzing, “-|” for inhibition, “-” for FIs extracted from complexes or inputs, and “---” for predicted FIs
Functional interaction (FI) between differently expressed genes that belongs to the “cellular lipid metabolic process”, “cellular response to lipid” and “fatty acid beta-oxidation” GO BP terms. In following figure “->” stands for activating/catalyzing, “-|” for inhibition, “-” for FIs extracted from complexes or inputs, and “---” for predicted FIs

Gene symbols, fold changes in expression, corrected p values and Entrez gene IDs of studied genes

GENE SYMBOLFOLD CHANGE D7/D1FOLD CHANGE D15/D1FOLD CHANGE D30/D1ADJUSTED P VALUE D7/D1ADJUSTED P VALUE D15/D1ADJUSTED P VALUE D30/D1
IL240,0833710,0621920,0713999,50E-063,21E-061,76E-06
CRABP20,1080460,0846380,0568013,11E-051,05E-052,84E-06
CYP24A10,1491690,2942760,0395842,69E-050,0001367,91E-07
SNAI20,1837660,1345210,1300790,0004110,0001297,85E-05
SPTLC30,1917860,5024610,3431723,75E-050,0016660,000124
KLF40,2054890,0899040,0574594,71E-053,93E-061,01E-06
MUC10,2407260,034740,021214,67E-059,76E-072,44E-07
CXCL100,2417130,4470190,3316340,0021650,0259030,004916
ERBB30,2702080,123880,1179984,67E-053,53E-061,48E-06
FKBP40,2912150,2911140,1922724,73E-052,82E-054,24E-06
ARSK5,7667114,5075231,321850,0001231,00E-051,73E-06
KITLG5,9755576,4783993,3021641,01E-053,68E-061,88E-05
P2RX76,1651428,5220425,7953982,69E-056,72E-061,03E-05
ABCA16,3133965,55674710,335583,95E-053,12E-054,36E-06
ACSS26,3497182,5033935,620937,45E-069,49E-052,39E-06
ACADL6,55257313,0754516,703856,68E-059,39E-063,46E-06
TNC6,572678,3523238,7734671,83E-054,91E-062,42E-06
PPARD9,2009736,8265225,6029912,25E-052,24E-052,49E-05
FADS39,51645510,2501912,300644,27E-061,47E-064,44E-07
LIPG36,546919,6781539,018012,50E-061,39E-062,57E-07
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Life Sciences, Molecular Biology, Biochemistry
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