<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Nerve agents are highly toxic organophosphorus (OP) compounds. They inhibit acetylcholinesterase (AChE), an enzyme that hydrolyses acetycholine (ACh) in the nervous system. Pathophysiological changes caused by OP poisonings are primarily the consequence of surplus ACh on cholinergic receptors and in the central nervous system. Standard treatment of OP poisoning includes combined administration of carbamates, atropine, oximes and anticonvulsants. In order to improve therapy, new compounds have been synthesised and tested. Tenocyclidine (TCP) and its adamantane derivative 1-[2-(2-thienyl)-2-adamantyl] morpholine (TAMORF) have shown interesting properties against soman poisoning. In this study, we developed a qualitative GC-MS method to measure elimination of TCP and TAMORF through rat urine in order to learn more about the mechanisms through which TCP protects an organism from OP poisoning and to determine the duration of this protective effect. GC-MS showed that six hours after treatment with TCP, rat urine contained only its metabolite 1-thienylcyclohexene, while urine of rats treated with TAMORF contained both TAMORF and its metabolites.</p></abstract>

Nerve agents are highly toxic organophosphorus (OP) compounds. They inhibit acetylcholinesterase (AChE), an enzyme that hydrolyses acetycholine (ACh) in the nervous system. Pathophysiological changes caused by OP poisonings are primarily the consequence of surplus ACh on cholinergic receptors and in the central nervous system. Standard treatment of OP poisoning includes combined administration of carbamates, atropine, oximes and anticonvulsants. In order to improve therapy, new compounds have been synthesised and tested. Tenocyclidine (TCP) and its adamantane derivative 1-[2-(2-thienyl)-2-adamantyl] morpholine (TAMORF) have shown interesting properties against soman poisoning. In this study, we developed a qualitative GC-MS method to measure elimination of TCP and TAMORF through rat urine in order to learn more about the mechanisms through which TCP protects an organism from OP poisoning and to determine the duration of this protective effect. GC-MS showed that six hours after treatment with TCP, rat urine contained only its metabolite 1-thienylcyclohexene, while urine of rats treated with TAMORF contained both TAMORF and its metabolites.

Qualitative GC-MS Assessment of TCP and Tamorf Elimination in Rats

Institute for Medical Research and Occupational Health, Zagreb, Croatia

Archives of Industrial Hygiene and Toxicology

{"article-title":"Qualitative GC-MS Assessment of TCP and Tamorf Elimination in Rats"}

Nerve agents are highly toxic organophosphorus (OP) compounds. They inhibit acetylcholinesterase (AChE), an enzyme that hydrolyses acetycholine (ACh) in the...

Qualitative GC-MS Assessment of TCP and Tamorf Elimination in Rats

Publié en ligne: 25 mars 2010

Pages: 61 - 67

DOI: https://doi.org/10.2478/10004-1254-61-2010-1985

Mots clés
antidot, kvalitativna metoda, metabolit, mokraća, tenociklidin

This content is open access.

Qualitative GC-MS Assessment of TCP and Tamorf Elimination in Rats

Publié en ligne: 25 mars 2010

Pages: 61 - 67

DOI: https://doi.org/10.2478/10004-1254-61-2010-1985

Mots clésantidot, kvalitativna metoda, metabolit, mokraća, tenociklidin

This content is open access.

Mots clés
antidot, kvalitativna metoda, metabolit, mokraća, tenociklidin