<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">Endogenous cell metabolism and different chemicals, drugs, ionising radiation, solar light, cigarette smoking, and air pollution can induce oxidative damage to DNA. Oxidative DNA damage is involved in the development of different diseases, aging, and cancer. Urine 8-hydroxy-2'deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the "whole body" oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in spot urine samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 50 nmol L-1 to 400 nmol L-1, the calibration curve was linear (r2=0.9995) and the limit of detection was 3 nmol L-1. Concentrations of 8-oxodG ranged between 3.0 nmol L-1 and 48.0 nmol L-1, which is in accordance with values reported for healthy individuals in other studies.</abstract>

Endogenous cell metabolism and different chemicals, drugs, ionising radiation, solar light, cigarette smoking, and air pollution can induce oxidative damage to DNA. Oxidative DNA damage is involved in the development of different diseases, aging, and cancer. Urine 8-hydroxy-2'deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the "whole body" oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in spot urine samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 50 nmol L-1 to 400 nmol L-1, the calibration curve was linear (r2=0.9995) and the limit of detection was 3 nmol L-1. Concentrations of 8-oxodG ranged between 3.0 nmol L-1 and 48.0 nmol L-1, which is in accordance with values reported for healthy individuals in other studies.

Determination of 8-hydroxy-2'deoxyguanosine in Urine Using HPLC With Electrochemical Detection

Institute for Medical Research and Occupational Health Zagreb, Croatia

Archives of Industrial Hygiene and Toxicology

Endogenous cell metabolism and different chemicals, drugs, ionising radiation, solar light, cigarette smoking, and air pollution can induce oxidative damage to...

Determination of 8-hydroxy-2'deoxyguanosine in Urine Using HPLC With Electrochemical Detection

Publié en ligne: 08 déc. 2008

Pages: 277 - 282

DOI: https://doi.org/10.2478/10004-1254-59-2008-1879

Mots clés
biomarker, oksidacijsko oštećenje DNA, 8-oksodG, slobodni radikali

This content is open access.

Determination of 8-hydroxy-2'deoxyguanosine in Urine Using HPLC With Electrochemical Detection

Publié en ligne: 08 déc. 2008

Pages: 277 - 282

DOI: https://doi.org/10.2478/10004-1254-59-2008-1879

Mots clésbiomarker, oksidacijsko oštećenje DNA, 8-oksodG, slobodni radikali

This content is open access.

Mots clés
biomarker, oksidacijsko oštećenje DNA, 8-oksodG, slobodni radikali