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A Novel Approach to Study the Effect of Ciprofloxacin on Biofilms of Corynebacterium spp. Using Confocal Laser Scanning Microscopy

ALINA OLENDER
ALINA OLENDER
, 
AGNIESZKA BOGUT
AGNIESZKA BOGUT
, 
AGNIESZKA MAGRYŚ
AGNIESZKA MAGRYŚ
 et 
KATARZYNA KRÓL-TURMIŃSKA
KATARZYNA KRÓL-TURMIŃSKA
   | 10 déc. 2018
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Article Category: original-paper
Publié en ligne: 10 déc. 2018
Pages: 431 - 440
Reçu: 28 mai 2018
Accepté: 12 juil. 2018
DOI: https://doi.org/10.21307/pjm-2018-051
Mots clés
© 2018 Alina Olender et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

The biofilms on the surface of the vascular catheter after 48 hours of incubation, stained with SYTO-13 with a marked height of the visible structures, A – C. striatum ATCC 6940, B – C. amycolatum ATCC 700207 (above 50×).
The biofilms on the surface of the vascular catheter after 48 hours of incubation, stained with SYTO-13 with a marked height of the visible structures, A – C. striatum ATCC 6940, B – C. amycolatum ATCC 700207 (above 50×).

Fig. 2.

The cross-section of the biofilm produced on the surface of the vascular catheter after 48 hours, stained with SYTO-13 – C. striatum ATCC 6940 (above 50×).
The cross-section of the biofilm produced on the surface of the vascular catheter after 48 hours, stained with SYTO-13 – C. striatum ATCC 6940 (above 50×).

Fig. 3.

The biofilm produced on the surface of the urological catheter after 48 hours of incubation, stained with SYTO-13 with a marked height of the visible structures (above 50×), A – C. striatum ATCC 6940, B – C. amycolatum ATCC 700207.
The biofilm produced on the surface of the urological catheter after 48 hours of incubation, stained with SYTO-13 with a marked height of the visible structures (above 50×), A – C. striatum ATCC 6940, B – C. amycolatum ATCC 700207.

Fig. 4.

The cross-section of the biofilm produced on the surface of the urological catheter after 48 hours of incubation, stained with SYTO-13 – C. amycolatum ATCC 700207 (above 50×).
The cross-section of the biofilm produced on the surface of the urological catheter after 48 hours of incubation, stained with SYTO-13 – C. amycolatum ATCC 700207 (above 50×).

Fig. 5.

The biofilm produced on the surface of the glass after 48 hours of incubation, stained with SYTO-13 with a marked height of the visible structures C. striatum ATCC 6940 (above 50×).
The biofilm produced on the surface of the glass after 48 hours of incubation, stained with SYTO-13 with a marked height of the visible structures C. striatum ATCC 6940 (above 50×).

Fig. 6.

Biofilms exposed to ciprofloxacin (MIC2 = 0,25 µg/l; MIC4 = 0,5 µg/l; MIC6 = 0,75 µg/l and control samples without antibiotic) – biofilm surface appearance: dead bacterial cells (red) and live bacterial cells (green) (above 200×).
Biofilms exposed to ciprofloxacin (MIC2 = 0,25 µg/l; MIC4 = 0,5 µg/l; MIC6 = 0,75 µg/l and control samples without antibiotic) – biofilm surface appearance: dead bacterial cells (red) and live bacterial cells (green) (above 200×).

Fig. 7.

The area occupied by the live bacteria forming the biofilm after treatment with ciprofloxacin at three concentrations (MIC2 = 0,25 µg/l; MIC4 = 0,5 µg/l; MIC6 = 0,75 µg/l) and control samples without the antibiotic.
The area occupied by the live bacteria forming the biofilm after treatment with ciprofloxacin at three concentrations (MIC2 = 0,25 µg/l; MIC4 = 0,5 µg/l; MIC6 = 0,75 µg/l) and control samples without the antibiotic.

Fig. 8.

The percentage of the biofilm surface with live bacteria after exposure to ciprofloxacin at three concentrations (MIC2 = 0,25 µg/l; MIC4 = 0,5 µg/l; MIC6 = 0,75 µg/l) and control samples without the antibiotic.
The percentage of the biofilm surface with live bacteria after exposure to ciprofloxacin at three concentrations (MIC2 = 0,25 µg/l; MIC4 = 0,5 µg/l; MIC6 = 0,75 µg/l) and control samples without the antibiotic.

Fig. 9.

The ratio of the area occupied by the live bacteria to the surface of dead bacteria within the biofilm after treatment with ciprofloxacin at three concentrations (MIC2 = 0,25 µg/l; MIC4 = 0,5 µg/l; MIC6 = 0,75 µg/l) and control samples without the antibiotic.
The ratio of the area occupied by the live bacteria to the surface of dead bacteria within the biofilm after treatment with ciprofloxacin at three concentrations (MIC2 = 0,25 µg/l; MIC4 = 0,5 µg/l; MIC6 = 0,75 µg/l) and control samples without the antibiotic.

Fig. 10.

The ratio of light intensity of live bacteria to dead bacteria after treatment with ciprofloxacin at three concentrations (MIC2 = 0,25 µg/l; MIC4 = 0,5 µg/l; MIC6 = 0,75 µg/l) and control samples without the antibiotic.
The ratio of light intensity of live bacteria to dead bacteria after treatment with ciprofloxacin at three concentrations (MIC2 = 0,25 µg/l; MIC4 = 0,5 µg/l; MIC6 = 0,75 µg/l) and control samples without the antibiotic.
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