<abstract xmlns="http://www.w3.org/1999/xhtml"><p>We isolated three laccase-producing fungus strains from <italic>Taxus</italic> rhizosphere. <italic>Myrotheium verrucaria</italic> strain DJTU-sh7 had the highest laccase activity of 216.2 U/ml, which was increased to above 300 U/ml after optimization. DJTU-sh7 had the best decolorizing effect for three classes of reactive dyes. The DJTU-sh7-containing fungal consortium displayed the robust decolorizing ability. Both color removal efficiency and chemical oxygen demand were increased in the consortium mediated biotransformation. Transcriptome changes of <italic>M. verrucaria</italic> elicited by azo dye and phenolic were quantified by the high throughput transcriptome sequencing, and the activities of the selected oxidases and reductases were determined. The possible involvement of oxidases and reductases, especially laccase, aryl alcohol oxidase, and ferric reductase in the biotransformation of dye and phenolic compounds was revealed at both transcriptomic and phenotypic levels. Revealing the transcriptomic mechanisms of fungi in dealing with organic pollutants facilitates the fine-tuned manipulation of strains in developing novel bioremediation and biodegradation strategies.</p></abstract>

We isolated three laccase-producing fungus strains from Taxus rhizosphere. Myrotheium verrucaria strain DJTU-sh7 had the highest laccase activity of 216.2 U/ml, which was increased to above 300 U/ml after optimization. DJTU-sh7 had the best decolorizing effect for three classes of reactive dyes. The DJTU-sh7-containing fungal consortium displayed the robust decolorizing ability. Both color removal efficiency and chemical oxygen demand were increased in the consortium mediated biotransformation. Transcriptome changes of M. verrucaria elicited by azo dye and phenolic were quantified by the high throughput transcriptome sequencing, and the activities of the selected oxidases and reductases were determined. The possible involvement of oxidases and reductases, especially laccase, aryl alcohol oxidase, and ferric reductase in the biotransformation of dye and phenolic compounds was revealed at both transcriptomic and phenotypic levels. Revealing the transcriptomic mechanisms of fungi in dealing with organic pollutants facilitates the fine-tuned manipulation of strains in developing novel bioremediation and biodegradation strategies.

Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from <italic>Taxus</italic> Rhizosphere

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from  Rhizosphere

We isolated three laccase-producing fungus strains from Taxus rhizosphere. Myrotheium verrucaria strain DJTU-sh7 had the highest laccase activity of 216.2...

Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

Article Category: original-paper

Publié en ligne: 10 déc. 2018

Pages: 417 - 430

Reçu: 19 avr. 2018

Accepté: 10 juil. 2018

DOI: https://doi.org/10.21307/pjm-2018-050

Mots clés
reactive dye decolorization, laccase, transcriptome sequencing, degradation mechanism

© 2018 Da Cheng Hao et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

Article Category: original-paper

Publié en ligne: 10 déc. 2018

Pages: 417 - 430

Reçu: 19 avr. 2018

Accepté: 10 juil. 2018

DOI: https://doi.org/10.21307/pjm-2018-050

Mots clésreactive dye decolorization, laccase, transcriptome sequencing, degradation mechanism

© 2018 Da Cheng Hao et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
reactive dye decolorization, laccase, transcriptome sequencing, degradation mechanism