Bacterial strains | Number of isolates (n) | ||||||||
---|---|---|---|---|---|---|---|---|---|
The reference method | Chromogenic media | ||||||||
Family or genus (n) | Species (by VITEK 2 Compact) and isolates number | Columbia Agar + 5% sheep blood (COL) | MacConkey Agar (MAC) | ChromID® CPS® Elite (CPSE, bioMérieux) | BD CHROMagar Orientation Medium (ORI, Becton Dickinson) | Brillance UTI Clarity Agar (UTI C, Oxoid) | CHROMagar™ Orientation (BioMaxima) | CHROMagar™ Orientation (ORIE, Graso) | |
Gram-negative rods (n = 62) | |||||||||
Enterobacteriaceae (n = 61) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
33 | 32 | 31 | 32 | 30 | 33 | 31 | 33 | ||
16 | 16 | 15 | 16 | 16 | 16 | 16 | 16 | ||
1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | ||
6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | ||
1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
Non-fermenting rods (n = 1) | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | |
Gram-positive cocci (n = 47) | |||||||||
Enterococci (n = 22) | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | |
20 | 20 | 0 | 18 | 20 | 18 | 19 | 20 | ||
1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | ||
Staphylococci (n = 21) | 9 | 9 | 0 | 8 | 6 | 8 | 8 | 9 | |
4 | 4 | 0 | 4 | 3 | 3 | 3 | 3 | ||
7 | 7 | 0 | 6 | 6 | 6 | 6 | 6 | ||
1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | ||
Streptococci (n = 4) | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | |
3 | 3 | 0 | 3 | 3 | 3 | 3 | 3 |
Parameters | Chromogenic media | |||||
---|---|---|---|---|---|---|
ChromID® CPS® Elite (CPSE, bioMérieux) | BD CHROMagar Orientation Medium (ORI, Becton Dickinson) | Brillance UTI Clarity Agar (UTI C, Oxoid) | CHROMagarTM Orientation (BioMaxima) | CHROMagarTM Orientation (ORIE, Graso) | Mean value | |
Qualitative cultures for all detected pathogens (p > 0.99) | ||||||
Sensitivity (%) | 94.7 | 91.7 | 89.6 | 94.4 | 97.2 | 93.5 |
Specificity (%) | 97.7 | 95.5 | 97.7 | 97.7 | 97.7 | 97.3 |
Positive predictive value (PPV) (%) | 98.9 | 98.0 | 98.4 | 99.0 | 99.1 | 98.7 |
Negative predictive value (NPV) (%) | 89.4 | 82.4 | 85.7 | 87.8 | 93.3 | 87.7 |
Qualitative cultures of | ||||||
Sensitivity (%) | 96.8 | 90.1 | 100 | 93.3 | 100 | 96.0 |
Semi-quantitative cultures for all detected pathogens (p = 0.9) | ||||||
Sensitivity (%) | 85.2 | 80.2 | 75.0 | 81.0 | 85.1 | 81.3 |
Specificity (%) | 85.7 | 82.4 | 91.3 | 82.7 | 82.4 | 84.9 |
Positive predictive value (PPV) (%) | 91.5 | 90.0 | 92.3 | 90.0 | 90.5 | 90.9 |
Negative predictive value (NPV) (%) | 76.4 | 67.7 | 72.4 | 69.4 | 73.7 | 71.9 |
Semi-quantitative cultures of | ||||||
Sensitivity (%) | 93.1 | 84.0 | 95.2 | 87.1 | 93.6 | 90.6 |
Number of isolates in a clinical specimen | Number of clinical specimens (urine) | Number of all isolates in all clinical specimens | Total number of isolates (% of the number cultured on reference media) | ||||
---|---|---|---|---|---|---|---|
Chromogenic media | |||||||
ChromID CPS Elite (CPSE, bioMérieux) | BD CHROMagar Orientation Medium (ORI, Becton Dickinson) | Brillance UTI Clarity Agar (UTI C, Oxoid) | CHROMagarTM Orientation (BioMaxima) | CHROMagarTM Orientation (ORIE, Graso) | |||
1 | 31 | 31 | 30 (96.8%) | 29 (93.6%) | 31 (100%) | 30 (96.6%) | 31 (100%) |
2 | 12 | 24 | 23 (95.8%) | 22 (91.7%) | 22 (91.7%) | 23 (95.8%) | 24 (100%) |
3 | 7 | 21 | 21 (100%) | 18 (85.7%) | 17 (81.0%) | 19 (90.5%) | 20 (95.3%) |
4 | 7 | 28 | 25 (89.3%) | 25 (89.3%) | 27 (96.4%) | 25 (89.3%) | 26 (92.6%) |
5 | 1 | 5 | 5 (100%) | 5 (100%) | 5 (100%) | 5 (100%) | 5 (100%) |